CPLM 1.0 - Compendium of Protein Lysine Modification
TagContent
CPLM ID CPLM-038226
UniProt Accession
Genbank Protein ID
Genbank Nucleotide ID
  
Protein Name
 Ubiquitin carboxyl-terminal hydrolase 
Protein Synonyms/Alias
  
Gene Name
 Usp14 
Gene Synonyms/Alias
  
Created Date
 July 27, 2013 
Organism
 Mus musculus (Mouse) 
NCBI Taxa ID
 10090 
Lysine Modification
Position
Peptide
Type
References
14TVKWGKEKFEGVELNubiquitination[1]
49ARQKVMVKGGTLKDDubiquitination[1]
62DDDWGNIKMKNMELPubiquitination[1]
95IRSVPELKDALKRYAubiquitination[1]
179MMRVLQQKLEAIEDDacetylation[2]
179MMRVLQQKLEAIEDDubiquitination[1]
203APAVTPSKKKSLIDQubiquitination[1]
256KYLFTGLKLRLQEEIacetylation[2]
256KYLFTGLKLRLQEEIubiquitination[1]
265RLQEEITKQSPTLQRubiquitination[1]
278QRNALYIKSSKISRLacetylation[2]
278QRNALYIKSSKISRLubiquitination[1]
307EKESVNAKVLKDVKFubiquitination[1]
413RKQDEWIKFDDDKVSubiquitination[1]
418WIKFDDDKVSIVTPEubiquitination[1]
Reference
 [1] Proteomic analyses reveal divergent ubiquitylation site patterns in murine tissues.
 Wagner SA, Beli P, Weinert BT, Schölz C, Kelstrup CD, Young C, Nielsen ML, Olsen JV, Brakebusch C, Choudhary C.
 Mol Cell Proteomics. 2012 Dec;11(12):1578-85. [PMID: 22790023]
 [2] Quantitative acetylome analysis reveals the roles of SIRT1 in regulating diverse substrates and cellular pathways.
 Chen Y, Zhao W, Yang JS, Cheng Z, Luo H, Lu Z, Tan M, Gu W, Zhao Y.
 Mol Cell Proteomics. 2012 Oct;11(10):1048-62. [PMID: 22826441] 
Functional Description
  
Sequence Annotation
  
Keyword
 Complete proteome; Hydrolase; Protease; Reference proteome; Thiol protease; Ubl conjugation pathway. 
Sequence Source
 UniProt (SWISSPROT/TrEMBL); GenBank; EMBL 
Protein Length
 458 AA 
Protein Sequence
MPLYSVTVKW GKEKFEGVEL NTDEPPMVFK AQLFALTGVQ PARQKVMVKG GTLKDDDWGN 60
IKMKNMELPC GLTNLGNTCY MNATVQCIRS VPELKDALKR YAGALRASGE MASAQYITAA 120
LRDLFDSMDK TSSSIPPIIL LQFLHMAFPQ FAEKGEQGQY LQQDANECWI QMMRVLQQKL 180
EAIEDDSGRE TDSSSAPAVT PSKKKSLIDQ YFGVEFETTM KCTESEEEEV TKGKENQLQL 240
SCFINQEVKY LFTGLKLRLQ EEITKQSPTL QRNALYIKSS KISRLPAYLT IQMVRFFYKE 300
KESVNAKVLK DVKFPLMLDV YELCTPELQE KMVSFRSKFK DLEDKKVNQQ PNANDKNSPP 360
KEIKYEPFSF ADDIGSNNCG YYDLQAVLTH QGRSSSSGHY VSWVRRKQDE WIKFDDDKVS 420
IVTPEDILRL SGGGDWHIAY VLLYGPRRVE IMEEESEQ 458 
Gene Ontology
 GO:0045202; C:synapse; IDA:MGI.
 GO:0008234; F:cysteine-type peptidase activity; IEA:UniProtKB-KW.
 GO:0004221; F:ubiquitin thiolesterase activity; IEA:InterPro.
 GO:0007268; P:synaptic transmission; IMP:MGI.
 GO:0006511; P:ubiquitin-dependent protein catabolic process; IEA:InterPro. 
Interpro
 IPR018200; Pept_C19ubi-hydrolase_C_CS.
 IPR001394; Peptidase_C19.
 IPR000626; Ubiquitin.
 IPR019954; Ubiquitin_CS. 
Pfam
 PF00443; UCH 
SMART
 SM00213; UBQ 
PROSITE
 PS00299; UBIQUITIN_1
 PS00972; UCH_2_1
 PS00973; UCH_2_2
 PS50235; UCH_2_3 
PRINTS