CPLM 1.0 - Compendium of Protein Lysine Modification
TagContent
CPLM ID CPLM-044309
UniProt Accession
Genbank Protein ID
Genbank Nucleotide ID
  
Protein Name
 Proteasome-associated protein ECM29 homolog 
Protein Synonyms/Alias
  
Gene Name
 KIAA0368 
Gene Synonyms/Alias
  
Created Date
 July 27, 2013 
Organism
 Homo sapiens (Human) 
NCBI Taxa ID
 9606 
Lysine Modification
Position
Peptide
Type
References
218FLPPVLLKLSSTQEGubiquitination[1, 2]
399GMSFYAAKRVIGDNPubiquitination[3]
416PEQLEQCKLGIVKFIubiquitination[2, 3]
457TAADLELKSKQSLIDubiquitination[3, 4]
459ADLELKSKQSLIDWNubiquitination[2]
473NNPAIINKMYKVYLGubiquitination[2]
485YLGDIPLKTKEGAVLubiquitination[1, 2, 3, 4, 5]
487GDIPLKTKEGAVLKPubiquitination[2, 6]
508VSTRVKLKIVPHLLRubiquitination[2]
582TKLINEYKEDPKLLSubiquitination[2]
586NEYKEDPKLLSMAYSubiquitination[1]
663LVASYLIKPEVQVRQubiquitination[3]
674QVRQVAVKFASTVFPubiquitination[1, 2, 7]
739MVYYIQEKASHRMKTubiquitination[3]
1009SEGSGFTKLHLVESLubiquitination[3, 4, 5, 6]
1024LSRIPSSKETNKMKEubiquitination[4]
1028PSSKETNKMKERAIQubiquitination[4]
1211LMTGKRVKHEVSGETubiquitination[2]
1228FQGGALGKTPDGQGLubiquitination[2, 3, 4, 5]
1239GQGLSTYKELCSLASubiquitination[3]
1271HAMWNSRKGAAFGFNubiquitination[2]
1496LRATEQEKAAMDSARubiquitination[2]
1590DRNSVIQKSCAFAMGubiquitination[3]
1610SRDSSTEKLLQKLNGacetylation[8, 9]
1614STEKLLQKLNGWYMEacetylation[9]
1614STEKLLQKLNGWYMEubiquitination[3]
1622LNGWYMEKEEPIYKTacetylation[9]
1622LNGWYMEKEEPIYKTubiquitination[3]
Reference
 [1] A proteome-wide, quantitative survey of in vivo ubiquitylation sites reveals widespread regulatory roles.
 Wagner SA, Beli P, Weinert BT, Nielsen ML, Cox J, Mann M, Choudhary C.
 Mol Cell Proteomics. 2011 Oct;10(10):M111.013284. [PMID: 21890473]
 [2] Refined preparation and use of anti-diglycine remnant (K-ε-GG) antibody enables routine quantification of 10,000s of ubiquitination sites in single proteomics experiments.
 Udeshi ND, Svinkina T, Mertins P, Kuhn E, Mani DR, Qiao JW, Carr SA.
 Mol Cell Proteomics. 2013 Mar;12(3):825-31. [PMID: 23266961]
 [3] Global identification of modular cullin-RING ligase substrates.
 Emanuele MJ, Elia AE, Xu Q, Thoma CR, Izhar L, Leng Y, Guo A, Chen YN, Rush J, Hsu PW, Yen HC, Elledge SJ.
 Cell. 2011 Oct 14;147(2):459-74. [PMID: 21963094]
 [4] Landscape of the PARKIN-dependent ubiquitylome in response to mitochondrial depolarization.
 Sarraf SA, Raman M, Guarani-Pereira V, Sowa ME, Huttlin EL, Gygi SP, Harper JW.
 Nature. 2013 Apr 18;496(7445):372-6. [PMID: 23503661]
 [5] Systematic and quantitative assessment of the ubiquitin-modified proteome.
 Kim W, Bennett EJ, Huttlin EL, Guo A, Li J, Possemato A, Sowa ME, Rad R, Rush J, Comb MJ, Harper JW, Gygi SP.
 Mol Cell. 2011 Oct 21;44(2):325-40. [PMID: 21906983]
 [6] Integrated proteomic analysis of post-translational modifications by serial enrichment.
 Mertins P, Qiao JW, Patel J, Udeshi ND, Clauser KR, Mani DR, Burgess MW, Gillette MA, Jaffe JD, Carr SA.
 Nat Methods. 2013 Jul;10(7):634-7. [PMID: 23749302]
 [7] Proteome-wide identification of ubiquitylation sites by conjugation of engineered lysine-less ubiquitin.
 Oshikawa K, Matsumoto M, Oyamada K, Nakayama KI.
 J Proteome Res. 2012 Feb 3;11(2):796-807. [PMID: 22053931]
 [8] Proteomic investigations reveal a role for RNA processing factor THRAP3 in the DNA damage response.
 Beli P, Lukashchuk N, Wagner SA, Weinert BT, Olsen JV, Baskcomb L, Mann M, Jackson SP, Choudhary C.
 Mol Cell. 2012 Apr 27;46(2):212-25. [PMID: 22424773]
 [9] Proteomic investigations of lysine acetylation identify diverse substrates of mitochondrial deacetylase sirt3.
 Sol EM, Wagner SA, Weinert BT, Kumar A, Kim HS, Deng CX, Choudhary C.
 PLoS One. 2012;7(12):e50545. [PMID: 23236377
Functional Description
  
Sequence Annotation
  
Keyword
 Complete proteome; Reference proteome. 
Sequence Source
 UniProt (SWISSPROT/TrEMBL); GenBank; EMBL 
Protein Length
 2017 AA 
Protein Sequence
MTSRTKRRQK VKISISKINH SSYRKEHRPF WEHQPPPHQN WAVGGRCGAG ARPGLSQRRG 60
REVRREAQGG ASSRRLRARL YFSESTSLPL DRRGSPGPLG PAPHGLRRAA PRGPARRTPR 120
STALTSRRRA QRPRHGDRLR LRSEARPRAA GDWPCARGGA VRPGRPHRPP RVESARGAHA 180
GRAATDQLER VFLRLGHAET DEQLQNIISK FLPPVLLKLS STQEGVRKKV MELLVHLNKR 240
IKSRPKIQLP VETLLVQYQD PAAVSFVTNF TIIYVKMGYP RLPVEKQCEL APTLLTAMEG 300
KPQPQQDSLM HLLIPTLFHM KYPVESSKSA SPFNLAEKPK TVQLLLDFML DVLLMPYGYV 360
LNESQSRQNS SSAQGSSSNS GGGSGIPQPP PGMSFYAAKR VIGDNPWTPE QLEQCKLGIV 420
KFIEAEQVPE LEAVLHLVIA SSDTRHSVAT AADLELKSKQ SLIDWNNPAI INKMYKVYLG 480
DIPLKTKEGA VLKPELKRDP VSTRVKLKIV PHLLRSRQAA ETFPANIQVV YDGLFGTNTN 540
SKLRTLSLQF VHHICITCPE IKIKPLGPML LNGLTKLINE YKEDPKLLSM AYSAVGKLSS 600
RMPHLFTKDI ALVQQLFEAL CKEEPETRLA IQEALSMMVG AYSTLEGAQR TLMEALVASY 660
LIKPEVQVRQ VAVKFASTVF PSDHIPSRYL LLLAAGDPRE EVHGEAQRVL RCLPGRNRKE 720
STSEQMPSFP EMVYYIQEKA SHRMKTPVKY MTGTTVLPFN PAAFGEIVLY LRMCLAHSAG 780
VVPTSQSLAD MQDHAPAIGR YIRTLMSSGQ MAPSSSNKSG ETNPVQIYIG LLQQLLAGVG 840
GLPVMYCLLE AVSVYPEKLA TKFVDKTEWI KSLMNNSKEE MRELAALFYS VVVSTVSGNE 900
LKSMIEQLIK TTKDNHSPEI QHGSLLALGF TVGRYLAKKK MRMSEQQDLE RNADTLPDQE 960
ELIQSATETI GSFLDSTSPL LAIAACTALG EIGRNGPLPI PSEGSGFTKL HLVESLLSRI 1020
PSSKETNKMK ERAIQTLGYF PVGDGDFPHQ KLLLQGLMDS VEAKQIELQF TIGEAITSAA 1080
IGTSSVAARD AWQMTEEEYT PPAGAKVNDV VPWVLDVILN KHIISPNPHV RQAACIWLLS 1140
LVRKLSTHKE VKSHLKEIQS AFVSVLSEND ELSQDVASKG LGLVYELGNE QDQQELVSTL 1200
VETLMTGKRV KHEVSGETVV FQGGALGKTP DGQGLSTYKE LCSLASDLSQ PDLVYKFMNL 1260
ANHHAMWNSR KGAAFGFNVI ATRAGEQLAP FLPQLVPRLY RYQFDPNLGI RQAMTSIWNA 1320
LVTDKSMVDK YLKEILQDLV KNLTSNMWRV RESSCLALND LLRGRPLDDI IDKLPEIWET 1380
LFRVQDDIKE SVRKAAELAL KTLSKVCVKM CDPAKGAAGQ RTIAALLPCL LDKGMMSTVT 1440
EVRALSINTL VKISKSAGAM LKPHAPKLIP ALLESLSVLE PQVLNYLSLR ATEQEKAAMD 1500
SARLSAAKSS PMMETINMCL QYLDVSVLGE LVPRLCELIR SGVGLGTKGG CASVIVSLTT 1560
QCPQDLTPYS GKLMSALLSG LTDRNSVIQK SCAFAMGHLV RTSRDSSTEK LLQKLNGWYM 1620
EKEEPIYKTS CALTIHAIGR YSPDVLKNHA KEVLPLAFLG MHEIADEEKS EKEECNLWTE 1680
VWQENVPGSF GGIRLYLQEL ITITQKALQS QSWKMKAQGA IAMASIAKQT SSLVPPYLGM 1740
ILTALLQGLA GRTWAGKEEL LKAIACVVTA CSAELEKSVP NQPSTNEILQ AVLKECSKEN 1800
VKYKIVAISC AADILKATKE DRFQEFSNIV IPLIKKNSLE SSGVRTTKNE EENEKEKELQ 1860
LEYLLGAFES LGKAWPRNAE TQRCYRQELC KLMCERLKLS TWKVQLGVLQ SMNAFFQGLM 1920
LLEEEHADPE ALAEILLETC KSITYSLENK TYSSVRTEAL SVIELLLKKL EESKQWECLT 1980
SECRVLLIES LATMEPDSRP ELQEKAALLK KTLENLE 2017 
Gene Ontology
  
Interpro
 IPR011989; ARM-like.
 IPR016024; ARM-type_fold.
 IPR026827; ECM29/GCN1.
 IPR026826; ECM29_metazoa.
 IPR024372; Proteasome_stabil_ECM29. 
Pfam
 PF13001; Ecm29 
SMART
  
PROSITE
  
PRINTS