UniProt Accession

 WASH1_HUMAN; A8K0Z3 

Genbank Protein ID

 AK289708; AL928970 

Genbank Nucleotide ID

 BAF82397.1 

Protein Name

 WAS protein family homolog 1 

Protein Synonyms/Alias

 CXYorf1-like protein on chromosome 9; Protein FAM39E 

Gene Name

 WASH1 

Gene Synonyms/Alias

 FAM39E 

Created Date

 July 27, 2013 

Organism

 Homo sapiens (Human) 

NCBI Taxa ID

 9606 

Lysine Modification

Position	Peptide	Type	References
68	QVQAIGEKVSLAQAK	ubiquitination	[1, 2]
75	KVSLAQAKIEKIKGS	ubiquitination	[1]
138	DERALQEKLKDFPVC	ubiquitination	[1]
185	TTENLYKKYVFLDPL	ubiquitination	[1]
220	DAPLSISKREQLEQQ	ubiquitination	[1, 2, 3]

Reference

 [1] Refined preparation and use of anti-diglycine remnant (K-ε-GG) antibody enables routine quantification of 10,000s of ubiquitination sites in single proteomics experiments.
 Udeshi ND, Svinkina T, Mertins P, Kuhn E, Mani DR, Qiao JW, Carr SA.
 Mol Cell Proteomics. 2013 Mar;12(3):825-31. [PMID: 23266961]
 [2] Integrated proteomic analysis of post-translational modifications by serial enrichment.
 Mertins P, Qiao JW, Patel J, Udeshi ND, Clauser KR, Mani DR, Burgess MW, Gillette MA, Jaffe JD, Carr SA.
 Nat Methods. 2013 Jul;10(7):634-7. [PMID: 23749302]
 [3] Systematic and quantitative assessment of the ubiquitin-modified proteome.
 Kim W, Bennett EJ, Huttlin EL, Guo A, Li J, Possemato A, Sowa ME, Rad R, Rush J, Comb MJ, Harper JW, Gygi SP.
 Mol Cell. 2011 Oct 21;44(2):325-40. [PMID: 21906983] 

Functional Description

 Acts as a nucleation-promoting factor at the surface of endosomes, where it recruits and activates the Arp2/3 complex to induce actin polymerization, playing a key role in the fission of tubules that serve as transport intermediates during endosome sorting. 

Sequence Annotation

 DOMAIN 361 383 WH2.
 REGION 1 167 WHD1.  

Keyword

 Actin-binding; Complete proteome; Endosome; Membrane; Reference proteome; Transport. 

Sequence Source

 UniProt (SWISSPROT/TrEMBL); GenBank; EMBL 

Protein Length

 465 AA 

Protein Sequence

Gene Ontology

 GO:0005769; C:early endosome; IDA:UniProtKB.
 GO:0031901; C:early endosome membrane; IEA:UniProtKB-SubCell.
 GO:0055037; C:recycling endosome; ISS:UniProtKB.
 GO:0055038; C:recycling endosome membrane; IEA:UniProtKB-SubCell.
 GO:0071203; C:WASH complex; IDA:UniProtKB.
 GO:0043014; F:alpha-tubulin binding; IDA:UniProtKB.
 GO:0034314; P:Arp2/3 complex-mediated actin nucleation; ISS:UniProtKB.
 GO:0042147; P:retrograde transport, endosome to Golgi; IMP:UniProtKB. 

Interpro

 IPR021854; WASH_WASD. 

Pfam

 PF11945; WASH_WAHD 

SMART

  

PROSITE

 PS51082; WH2 

PRINTS