CPLM 1.0 - Compendium of Protein Lysine Modification
TagContent
CPLM ID CPLM-008056
UniProt Accession
Genbank Protein ID
Genbank Nucleotide ID
Protein Name
 Vacuolar-sorting protein BRO1 
Protein Synonyms/Alias
 Amino acid sensor-independent protein 6; BCK1-like resistance to osmotic shock protein 1; BRO domain-containing protein 1; Nitrogen permease inactivating protein 3; Vacuolar protein-sorting-associated protein 31 
Gene Name
 BRO1 
Gene Synonyms/Alias
 ASI6; LPF2; NPI3; VPS31; YPL084W 
Created Date
 July 27, 2013 
Organism
 Saccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast) 
NCBI Taxa ID
 559292 
Lysine Modification
Position
Peptide
Type
References
28GLSSYLKKSYGSSQWubiquitination[1]
289LISSLPFKTWLVEFIubiquitination[2]
Reference
 [1] Global analysis of phosphorylation and ubiquitylation cross-talk in protein degradation.
 Swaney DL, Beltrao P, Starita L, Guo A, Rush J, Fields S, Krogan NJ, Villén J.
 Nat Methods. 2013 Jul;10(7):676-82. [PMID: 23749301]
 [2] Identification, analysis, and prediction of protein ubiquitination sites.
 Radivojac P, Vacic V, Haynes C, Cocklin RR, Mohan A, Heyen JW, Goebl MG, Iakoucheva LM.
 Proteins. 2010 Feb 1;78(2):365-80. [PMID: 19722269] 
Functional Description
 Class E VPS protein involved in concentration and sorting of cargo proteins of the multivesicular body (MVB) for incorporation into intralumenal vesicles. Fusion between endosomes and the vacuole will then target the cargo proteins to the vacuolar lumen. Acts as an adapter that recruits the DOA4 deubiquitinase to the endosomes, leading to deubiquitination of cargo proteins prior to the lumenal sequestration. Its association to the endosomes depends on SNF7 and its dissociation requires VPS4. Interacts functionally with the Pkc1p-mitogen-activated protein kinase pathway. 
Sequence Annotation
 DOMAIN 4 400 BRO1.
 MOD_RES 740 740 Phosphoserine.  
Keyword
 3D-structure; Coiled coil; Complete proteome; Cytoplasm; Endosome; Phosphoprotein; Protein transport; Reference proteome; Transport. 
Sequence Source
 UniProt (SWISSPROT/TrEMBL); GenBank; EMBL 
Protein Length
 844 AA 
Protein Sequence
MKPYLFDLKL KDTEKLDWKK GLSSYLKKSY GSSQWRTFYD EKATSELDHL RNNANGELAP 60
SSLSEQNLKY YSFLEHLYFR LGSKGSRLKM DFTWYDAEYS SAQKGLKYTQ HTLAFEKSCT 120
LFNIAVIFTQ IARENINEDY KNSIANLTKA FSCFEYLSEN FLNSPSVDLQ SENTRFLANI 180
CHAEAQELFV LKLLNDQISS KQYTLISKLS RATCNLFQKC HDFMKEIDDD VAIYGEPKWK 240
TTVTCKLHFY KSLSAYYHGL HLEEENRVGE AIAFLDFSMQ QLISSLPFKT WLVEFIDFDG 300
FKETLEKKQK ELIKDNDFIY HESVPAVVQV DSIKALDAIK SPTWEKILEP YMQDVANKCD 360
SLYRGIIPLD VYEKESIYSE EKATLLRKQV EETETANLEY SSFIEFTNLP RLLSDLEKQF 420
SDGNIFSNTD TQGQLMRDQI QTWCKFIQTN EFRDIEEQMN KIVFKRKQIL EILSALPNDQ 480
KENVTKLKSS LVAASNSDEK LFACVKPHIV EINLLNDNGK IWKKFDEFNR NTPPQPSLLD 540
IDDTKNDKIL ELLKQVKGHA EDLRTLKEER SRNLSELRDE INNDDITKLL IINKGKSDVE 600
LKDLFEVELE KFEPLSTRIE ATIYKQSSMI DDIKAKLDEI FHLSNFKDKS SGEEKFLEDR 660
KNFFDKLQEA VKSFSIFASD LPKGIEFYDS LFNMSRDLAE RVRVAKQTED STANSPAPPL 720
PPLDSKASVV GGPPLLPQKS AAFQSLSRQG LNLGDQFQNL KISAGSDLPQ GPGIPPRTYE 780
ASPYAATPTM AAPPVPPKQS QEDMYDLRRR KAVENEEREL QENPTSFYNR PSVFDENMYS 840
KYSS 844 
Gene Ontology
 GO:0005768; C:endosome; IDA:SGD.
 GO:0016579; P:protein deubiquitination; IGI:SGD.
 GO:0043328; P:protein targeting to vacuole involved in ubiquitin-dependent protein catabolic process via the multivesicular body sorting pathway; IMP:SGD.
 GO:0007584; P:response to nutrient; IMP:SGD. 
Interpro
 IPR025304; ALIX_V_dom.
 IPR004328; BRO1_dom. 
Pfam
 PF13949; ALIX_LYPXL_bnd
 PF03097; BRO1 
SMART
 SM01041; BRO1 
PROSITE
 PS51180; BRO1 
PRINTS