CPLM 1.0 - Compendium of Protein Lysine Modification
TagContent
CPLM ID CPLM-022048
UniProt Accession
Genbank Protein ID
Genbank Nucleotide ID
Protein Name
 Sialic acid synthase 
Protein Synonyms/Alias
 N-acetylneuraminate synthase; N-acetylneuraminate-9-phosphate synthase; N-acetylneuraminic acid phosphate synthase; N-acetylneuraminic acid synthase 
Gene Name
 NANS 
Gene Synonyms/Alias
 SAS 
Created Date
 July 27, 2013 
Organism
 Homo sapiens (Human) 
NCBI Taxa ID
 9606 
Lysine Modification
Position
Peptide
Type
References
52ECGADCAKFQKSELEubiquitination[1, 2]
55ADCAKFQKSELEFKFubiquitination[1, 2, 3]
61QKSELEFKFNRKALEubiquitination[1]
79TSKHSWGKTYGEHKRacetylation[4]
145NNFPYLEKTAKKGRPubiquitination[1, 2, 3]
243ERHITLDKTWKGSDHubiquitination[1]
246ITLDKTWKGSDHSASubiquitination[3]
290CEMACNEKLGKSVVAacetylation[2, 4]
290CEMACNEKLGKSVVAubiquitination[1]
293ACNEKLGKSVVAKVKubiquitination[1]
Reference
 [1] Refined preparation and use of anti-diglycine remnant (K-ε-GG) antibody enables routine quantification of 10,000s of ubiquitination sites in single proteomics experiments.
 Udeshi ND, Svinkina T, Mertins P, Kuhn E, Mani DR, Qiao JW, Carr SA.
 Mol Cell Proteomics. 2013 Mar;12(3):825-31. [PMID: 23266961]
 [2] Integrated proteomic analysis of post-translational modifications by serial enrichment.
 Mertins P, Qiao JW, Patel J, Udeshi ND, Clauser KR, Mani DR, Burgess MW, Gillette MA, Jaffe JD, Carr SA.
 Nat Methods. 2013 Jul;10(7):634-7. [PMID: 23749302]
 [3] Systematic and quantitative assessment of the ubiquitin-modified proteome.
 Kim W, Bennett EJ, Huttlin EL, Guo A, Li J, Possemato A, Sowa ME, Rad R, Rush J, Comb MJ, Harper JW, Gygi SP.
 Mol Cell. 2011 Oct 21;44(2):325-40. [PMID: 21906983]
 [4] Lysine acetylation targets protein complexes and co-regulates major cellular functions.
 Choudhary C, Kumar C, Gnad F, Nielsen ML, Rehman M, Walther TC, Olsen JV, Mann M.
 Science. 2009 Aug 14;325(5942):834-40. [PMID: 19608861] 
Functional Description
 Produces N-acetylneuraminic acid (Neu5Ac) and 2-keto-3- deoxy-D-glycero-D-galacto-nononic acid (KDN). Can also use N- acetylmannosamine 6-phosphate and mannose 6-phosphate as substrates to generate phosphorylated forms of Neu5Ac and KDN, respectively. 
Sequence Annotation
 DOMAIN 294 353 AFP-like.
 MOD_RES 79 79 N6-acetyllysine.  
Keyword
 3D-structure; Acetylation; Complete proteome; Direct protein sequencing; Polymorphism; Reference proteome; Transferase. 
Sequence Source
 UniProt (SWISSPROT/TrEMBL); GenBank; EMBL 
Protein Length
 359 AA 
Protein Sequence
MPLELELCPG RWVGGQHPCF IIAEIGQNHQ GDLDVAKRMI RMAKECGADC AKFQKSELEF 60
KFNRKALERP YTSKHSWGKT YGEHKRHLEF SHDQYRELQR YAEEVGIFFT ASGMDEMAVE 120
FLHELNVPFF KVGSGDTNNF PYLEKTAKKG RPMVISSGMQ SMDTMKQVYQ IVKPLNPNFC 180
FLQCTSAYPL QPEDVNLRVI SEYQKLFPDI PIGYSGHETG IAISVAAVAL GAKVLERHIT 240
LDKTWKGSDH SASLEPGELA ELVRSVRLVE RALGSPTKQL LPCEMACNEK LGKSVVAKVK 300
IPEGTILTMD MLTVKVGEPK GYPPEDIFNL VGKKVLVTVE EDDTIMEELV DNHGKKIKS 359 
Gene Ontology
 GO:0005737; C:cytoplasm; NAS:UniProtKB.
 GO:0005829; C:cytosol; IEA:Compara.
 GO:0050462; F:N-acetylneuraminate synthase activity; IEA:EC.
 GO:0008781; F:N-acylneuraminate cytidylyltransferase activity; NAS:UniProtKB.
 GO:0047444; F:N-acylneuraminate-9-phosphate synthase activity; IEA:EC.
 GO:0009103; P:lipopolysaccharide biosynthetic process; NAS:UniProtKB. 
Interpro
 IPR006190; AFP_Neu5c_C.
 IPR013785; Aldolase_TIM.
 IPR006013; Antifreeze_III.
 IPR013132; Neu5Ac_N.
 IPR013974; SAF. 
Pfam
 PF03102; NeuB
 PF08666; SAF 
SMART
 SM00858; SAF 
PROSITE
 PS50844; AFP_LIKE 
PRINTS
 PR00357; ANTIFREEZIII.