UniProt Accession

 HLAE_HUMAN; P13747; Q30169; Q9BT83; Q9GIY7; Q9GIY8 

Genbank Protein ID

 M20022; AJ293263; AJ293264; AF523274; AF523275; AF523276; AF523277; AF523278; AF523279; AF523280; AF523281; AF523282; AF523283; BA000025; BC002578; BC004297; M21533; AY221103; AY216681; M32508 

Genbank Nucleotide ID

 AAA52655.1; CAC07212.1; CAC07213.1; AAM74969.1; AAM74970.1; AAM74971.1; AAM74972.1; AAM74973.1; AAM74974.1; AAM74975.1; AAM74976.1; AAM74977.1; AAM74978.1; BAB63328.1; AAH02578.1; AAH04297.1; AAA59835.1; AAO34408.1; AAO37688.1; AAA63225.1 

Protein Name

 HLA class I histocompatibility antigen, alpha chain E 

Protein Synonyms/Alias

 MHC class I antigen E 

Gene Name

 HLA-E 

Gene Synonyms/Alias

 HLA-6.2; HLAE 

Created Date

 July 27, 2013 

Organism

 Homo sapiens (Human) 

NCBI Taxa ID

 9606 

Lysine Modification

Position	Peptide	Type	References
142	EQFAYDGKDYLTLNE	ubiquitination	[1, 2, 3, 4]
167	AAQISEQKSNDASEA	ubiquitination	[1, 2, 3, 4]
191	TCVEWLHKYLEKGKE	ubiquitination	[3]
195	WLHKYLEKGKETLLH	ubiquitination	[3]
197	HKYLEKGKETLLHLE	ubiquitination	[1, 3, 4]
207	LLHLEPPKTHVTHHP	ubiquitination	[3]
337	RKKSSGGKGGSYSKA	ubiquitination	[1]
343	GKGGSYSKAEWSDSA	ubiquitination	[1, 2, 3, 4, 5, 6]

Reference

 [1] Systematic and quantitative assessment of the ubiquitin-modified proteome.
 Kim W, Bennett EJ, Huttlin EL, Guo A, Li J, Possemato A, Sowa ME, Rad R, Rush J, Comb MJ, Harper JW, Gygi SP.
 Mol Cell. 2011 Oct 21;44(2):325-40. [PMID: 21906983]
 [2] Global identification of modular cullin-RING ligase substrates.
 Emanuele MJ, Elia AE, Xu Q, Thoma CR, Izhar L, Leng Y, Guo A, Chen YN, Rush J, Hsu PW, Yen HC, Elledge SJ.
 Cell. 2011 Oct 14;147(2):459-74. [PMID: 21963094]
 [3] Refined preparation and use of anti-diglycine remnant (K-ε-GG) antibody enables routine quantification of 10,000s of ubiquitination sites in single proteomics experiments.
 Udeshi ND, Svinkina T, Mertins P, Kuhn E, Mani DR, Qiao JW, Carr SA.
 Mol Cell Proteomics. 2013 Mar;12(3):825-31. [PMID: 23266961]
 [4] Landscape of the PARKIN-dependent ubiquitylome in response to mitochondrial depolarization.
 Sarraf SA, Raman M, Guarani-Pereira V, Sowa ME, Huttlin EL, Gygi SP, Harper JW.
 Nature. 2013 Apr 18;496(7445):372-6. [PMID: 23503661]
 [5] A proteome-wide, quantitative survey of in vivo ubiquitylation sites reveals widespread regulatory roles.
 Wagner SA, Beli P, Weinert BT, Nielsen ML, Cox J, Mann M, Choudhary C.
 Mol Cell Proteomics. 2011 Oct;10(10):M111.013284. [PMID: 21890473]
 [6] Integrated proteomic analysis of post-translational modifications by serial enrichment.
 Mertins P, Qiao JW, Patel J, Udeshi ND, Clauser KR, Mani DR, Burgess MW, Gillette MA, Jaffe JD, Carr SA.
 Nat Methods. 2013 Jul;10(7):634-7. [PMID: 23749302] 

Functional Description

 Preferably binds to a peptide derived from the signal sequence of most HLA-A, -B, -C and -G molecules. 

Sequence Annotation

 DOMAIN 206 294 Ig-like C1-type.
 REGION 22 111 Alpha-1.
 REGION 112 203 Alpha-2.
 REGION 204 295 Alpha-3.
 REGION 296 305 Connecting peptide.
 CARBOHYD 107 107 N-linked (GlcNAc...).
 DISULFID 122 185
 DISULFID 224 280  

Keyword

 3D-structure; Complete proteome; Disulfide bond; Glycoprotein; Immunity; Membrane; MHC I; Polymorphism; Reference proteome; Signal; Transmembrane; Transmembrane helix. 

Sequence Source

 UniProt (SWISSPROT/TrEMBL); GenBank; EMBL 

Protein Length

 358 AA 

Protein Sequence

Gene Ontology

 GO:0031901; C:early endosome membrane; TAS:Reactome.
 GO:0012507; C:ER to Golgi transport vesicle membrane; TAS:Reactome.
 GO:0000139; C:Golgi membrane; TAS:Reactome.
 GO:0071556; C:integral to lumenal side of endoplasmic reticulum membrane; TAS:Reactome.
 GO:0042612; C:MHC class I protein complex; IEA:UniProtKB-KW.
 GO:0030670; C:phagocytic vesicle membrane; TAS:Reactome.
 GO:0005886; C:plasma membrane; TAS:Reactome.
 GO:0002479; P:antigen processing and presentation of exogenous peptide antigen via MHC class I, TAP-dependent; TAS:Reactome.
 GO:0002480; P:antigen processing and presentation of exogenous peptide antigen via MHC class I, TAP-independent; TAS:Reactome.
 GO:0060333; P:interferon-gamma-mediated signaling pathway; TAS:Reactome.
 GO:0050776; P:regulation of immune response; TAS:Reactome.
 GO:0060337; P:type I interferon-mediated signaling pathway; TAS:Reactome. 

Interpro

 IPR007110; Ig-like_dom.
 IPR013783; Ig-like_fold.
 IPR003006; Ig/MHC_CS.
 IPR003597; Ig_C1-set.
 IPR011161; MHC_I-like_Ag-recog.
 IPR011162; MHC_I/II-like_Ag-recog.
 IPR001039; MHC_I_a_a1/a2. 

Pfam

 PF07654; C1-set
 PF00129; MHC_I 

SMART

 SM00407; IGc1 

PROSITE

 PS50835; IG_LIKE
 PS00290; IG_MHC 

PRINTS

 PR01638; MHCCLASSI.