UniProt Accession

 KLHL7_HUMAN; Q8IXQ5; A4D144; B7Z5I9; G5E9G3; Q7Z765; Q96MV2; Q9BQF8; Q9UDQ9 

Genbank Protein ID

 AF111113; AL136597; AK056390; AK299006; EF560731; AC005082; AC006039; AC073992; CH236948; CH471073; CH471073; CH471073; BC009555; BC039585 

Genbank Nucleotide ID

 AAF27196.1; CAB66532.1; BAB71175.1; BAH12925.1; ABQ59041.1; AAO21916.1; EAL24261.1; EAW93770.1; EAW93773.1; EAW93774.1; AAH09555.1; AAH39585.1 

Protein Name

 Kelch-like protein 7 

Protein Synonyms/Alias

  

Gene Name

 KLHL7 

Gene Synonyms/Alias

  

Created Date

 July 27, 2013 

Organism

 Homo sapiens (Human) 

NCBI Taxa ID

 9606 

Lysine Modification

Position	Peptide	Type	References
130	QYQIEPVKKMCVDFL	ubiquitination	[1, 2]
188	EFLQLDVKRVTHLLN	ubiquitination	[1]
240	VRFPLISKNFLSKTV	ubiquitination	[2]
245	ISKNFLSKTVQAEPL	ubiquitination	[2, 3]
261	QDNPECLKMVISGMR	ubiquitination	[2]
312	SCRYFNPKDYSWTDI	ubiquitination	[2]
359	MDCYNVVKDSWYSKL	ubiquitination	[2]
365	VKDSWYSKLGPPTPR	ubiquitination	[2]
413	RTESWHTKPSMLTQR	ubiquitination	[1, 2]
472	CPMIEARKNHGLVFV	ubiquitination	[2]
480	NHGLVFVKDKIFAVG	ubiquitination	[2]
504	NVEYYDIKLNEWKMV	ubiquitination	[1]
517	MVSPMPWKGVTVKCA	ubiquitination	[2, 4, 5]
560	DKWVANSKVRAFPVT	ubiquitination	[2]

Reference

 [1] Systematic and quantitative assessment of the ubiquitin-modified proteome.
 Kim W, Bennett EJ, Huttlin EL, Guo A, Li J, Possemato A, Sowa ME, Rad R, Rush J, Comb MJ, Harper JW, Gygi SP.
 Mol Cell. 2011 Oct 21;44(2):325-40. [PMID: 21906983]
 [2] Refined preparation and use of anti-diglycine remnant (K-ε-GG) antibody enables routine quantification of 10,000s of ubiquitination sites in single proteomics experiments.
 Udeshi ND, Svinkina T, Mertins P, Kuhn E, Mani DR, Qiao JW, Carr SA.
 Mol Cell Proteomics. 2013 Mar;12(3):825-31. [PMID: 23266961]
 [3] Methods for quantification of in vivo changes in protein ubiquitination following proteasome and deubiquitinase inhibition.
 Udeshi ND, Mani DR, Eisenhaure T, Mertins P, Jaffe JD, Clauser KR, Hacohen N, Carr SA.
 Mol Cell Proteomics. 2012 May;11(5):148-59. [PMID: 22505724]
 [4] A proteome-wide, quantitative survey of in vivo ubiquitylation sites reveals widespread regulatory roles.
 Wagner SA, Beli P, Weinert BT, Nielsen ML, Cox J, Mann M, Choudhary C.
 Mol Cell Proteomics. 2011 Oct;10(10):M111.013284. [PMID: 21890473]
 [5] hCKSAAP_UbSite: improved prediction of human ubiquitination sites by exploiting amino acid pattern and properties.
 Chen Z, Zhou Y, Song J, Zhang Z.
 Biochim Biophys Acta. 2013 Aug;1834(8):1461-7. [PMID: 23603789] 

Functional Description

 Substrate-specific adapter of a BCR (BTB-CUL3-RBX1) E3 ubiquitin ligase complex. The BCR(KLHL7) complex acts by mediating ubiquitination and subsequent degradation of substrate proteins. Probably mediates 'Lys-48'-linked ubiquitination. 

Sequence Annotation

 DOMAIN 44 111 BTB.
 DOMAIN 146 248 BACK.
 REPEAT 294 336 Kelch 1.
 REPEAT 337 382 Kelch 2.
 REPEAT 383 430 Kelch 3.
 REPEAT 431 481 Kelch 4.
 REPEAT 483 528 Kelch 5.
 REPEAT 530 575 Kelch 6.  

Keyword

 3D-structure; Alternative splicing; Complete proteome; Disease mutation; Kelch repeat; Nucleus; Polymorphism; Reference proteome; Repeat; Retinitis pigmentosa; Ubl conjugation pathway. 

Sequence Source

 UniProt (SWISSPROT/TrEMBL); GenBank; EMBL 

Protein Length

 586 AA 

Protein Sequence

Gene Ontology

 GO:0031463; C:Cul3-RING ubiquitin ligase complex; IDA:UniProtKB.
 GO:0005737; C:cytoplasm; IDA:HPA.
 GO:0005730; C:nucleolus; IDA:LIFEdb.
 GO:0005886; C:plasma membrane; IDA:HPA.
 GO:0042803; F:protein homodimerization activity; IDA:UniProtKB.
 GO:0016567; P:protein ubiquitination; IDA:UniProtKB. 

Interpro

 IPR011705; BACK.
 IPR000210; BTB/POZ-like.
 IPR011333; BTB/POZ_fold.
 IPR013069; BTB_POZ.
 IPR017096; Kelch-like_gigaxonin.
 IPR015915; Kelch-typ_b-propeller.
 IPR006652; Kelch_1. 

Pfam

 PF07707; BACK
 PF00651; BTB
 PF01344; Kelch_1 

SMART

 SM00875; BACK
 SM00225; BTB
 SM00612; Kelch 

PROSITE

 PS50097; BTB 

PRINTS