CPLM 1.0 - Compendium of Protein Lysine Modification
TagContent
CPLM ID CPLM-030076
UniProt Accession
Genbank Protein ID
Genbank Nucleotide ID
Protein Name
 Chaperone protein DnaK 
Protein Synonyms/Alias
 HSP70; Heat shock 70 kDa protein; Heat shock protein 70 
Gene Name
 dnaK 
Gene Synonyms/Alias
 ECDH10B_0014 
Created Date
 July 27, 2013 
Organism
 Escherichia coli (strain K12 / DH10B) 
NCBI Taxa ID
 316385 
Lysine Modification
Position
Peptide
Type
References
70QNTLFAIKRLIGRRFsuccinylation[1]
245NYLVEEFKKDQGIDLsuccinylation[1]
246YLVEEFKKDQGIDLRsuccinylation[1]
304NIKVTRAKLESLVEDsuccinylation[1]
359KVAEFFGKEPRKDVNsuccinylation[1]
502KEQKITIKASSGLNEsuccinylation[1]
528ANAEADRKFEELVQTsuccinylation[1]
587DKAAIEAKMQELAQVsuccinylation[1]
Reference
 [1] Identification of lysine succinylation as a new post-translational modification.
 Zhang Z, Tan M, Xie Z, Dai L, Chen Y, Zhao Y.
 Nat Chem Biol. 2011 Jan;7(1):58-63. [PMID: 21151122] 
Functional Description
 Acts as a chaperone (By similarity). 
Sequence Annotation
 MOD_RES 109 109 N6-acetyllysine (By similarity).
 MOD_RES 199 199 Phosphothreonine; by autocatalysis (By
 MOD_RES 245 245 N6-acetyllysine (By similarity).
 MOD_RES 304 304 N6-acetyllysine (By similarity).
 MOD_RES 421 421 N6-acetyllysine (By similarity).  
Keyword
 Acetylation; ATP-binding; Chaperone; Complete proteome; Nucleotide-binding; Phosphoprotein; Stress response. 
Sequence Source
 UniProt (SWISSPROT/TrEMBL); GenBank; EMBL 
Protein Length
 638 AA 
Protein Sequence
MGKIIGIDLG TTNSCVAIMD GTTPRVLENA EGDRTTPSII AYTQDGETLV GQPAKRQAVT 60
NPQNTLFAIK RLIGRRFQDE EVQRDVSIMP FKIIAADNGD AWVEVKGQKM APPQISAEVL 120
KKMKKTAEDY LGEPVTEAVI TVPAYFNDAQ RQATKDAGRI AGLEVKRIIN EPTAAALAYG 180
LDKGTGNRTI AVYDLGGGTF DISIIEIDEV DGEKTFEVLA TNGDTHLGGE DFDSRLINYL 240
VEEFKKDQGI DLRNDPLAMQ RLKEAAEKAK IELSSAQQTD VNLPYITADA TGPKHMNIKV 300
TRAKLESLVE DLVNRSIEPL KVALQDAGLS VSDIDDVILV GGQTRMPMVQ KKVAEFFGKE 360
PRKDVNPDEA VAIGAAVQGG VLTGDVKDVL LLDVTPLSLG IETMGGVMTT LIAKNTTIPT 420
KHSQVFSTAE DNQSAVTIHV LQGERKRAAD NKSLGQFNLD GINPAPRGMP QIEVTFDIDA 480
DGILHVSAKD KNSGKEQKIT IKASSGLNED EIQKMVRDAE ANAEADRKFE ELVQTRNQGD 540
HLLHSTRKQV EEAGDKLPAD DKTAIESALT ALETALKGED KAAIEAKMQE LAQVSQKLME 600
IAQQQHAQQQ TAGADASANN AKDDDVVDAE FEEVKDKK 638 
Gene Ontology
 GO:0005524; F:ATP binding; IEA:HAMAP.
 GO:0006457; P:protein folding; IEA:InterPro.
 GO:0006950; P:response to stress; IEA:UniProtKB-KW. 
Interpro
 IPR012725; Chaperone_DnaK.
 IPR018181; Heat_shock_70_CS.
 IPR013126; Hsp_70_fam. 
Pfam
 PF00012; HSP70 
SMART
  
PROSITE
 PS00297; HSP70_1
 PS00329; HSP70_2
 PS01036; HSP70_3 
PRINTS
 PR00301; HEATSHOCK70.