CPLM 1.0 - Compendium of Protein Lysine Modification
TagContent
CPLM ID CPLM-000450
UniProt Accession
Genbank Protein ID
Genbank Nucleotide ID
Protein Name
 Glutamate receptor ionotropic, NMDA 2D 
Protein Synonyms/Alias
 GluN2D; EB11; Glutamate [NMDA] receptor subunit epsilon-4; N-methyl D-aspartate receptor subtype 2D; NMDAR2D; NR2D 
Gene Name
 GRIN2D 
Gene Synonyms/Alias
 GluN2D; NMDAR2D 
Created Date
 July 27, 2013 
Organism
 Homo sapiens (Human) 
NCBI Taxa ID
 9606 
Lysine Modification
Position
Peptide
Type
References
412EQQTLRLKYPLWSRYubiquitination[1]
516TNGKHGKKIDGVWNGubiquitination[2]
620NRSLATGKRPGGSTFubiquitination[2]
697VSGLSDRKFQRPQEQubiquitination[1, 2]
720VPNGSTEKNIRSNYPubiquitination[1, 2]
749EEALTQLKAGKLDAFubiquitination[1, 2, 3]
795GYGIALHKGSRWKRPubiquitination[1, 2, 4, 5]
1157GRYWSVDKLGGWRAGubiquitination[4, 5]
Reference
 [1] Landscape of the PARKIN-dependent ubiquitylome in response to mitochondrial depolarization.
 Sarraf SA, Raman M, Guarani-Pereira V, Sowa ME, Huttlin EL, Gygi SP, Harper JW.
 Nature. 2013 Apr 18;496(7445):372-6. [PMID: 23503661]
 [2] Systematic and quantitative assessment of the ubiquitin-modified proteome.
 Kim W, Bennett EJ, Huttlin EL, Guo A, Li J, Possemato A, Sowa ME, Rad R, Rush J, Comb MJ, Harper JW, Gygi SP.
 Mol Cell. 2011 Oct 21;44(2):325-40. [PMID: 21906983]
 [3] Global identification of modular cullin-RING ligase substrates.
 Emanuele MJ, Elia AE, Xu Q, Thoma CR, Izhar L, Leng Y, Guo A, Chen YN, Rush J, Hsu PW, Yen HC, Elledge SJ.
 Cell. 2011 Oct 14;147(2):459-74. [PMID: 21963094]
 [4] A proteome-wide, quantitative survey of in vivo ubiquitylation sites reveals widespread regulatory roles.
 Wagner SA, Beli P, Weinert BT, Nielsen ML, Cox J, Mann M, Choudhary C.
 Mol Cell Proteomics. 2011 Oct;10(10):M111.013284. [PMID: 21890473]
 [5] hCKSAAP_UbSite: improved prediction of human ubiquitination sites by exploiting amino acid pattern and properties.
 Chen Z, Zhou Y, Song J, Zhang Z.
 Biochim Biophys Acta. 2013 Aug;1834(8):1461-7. [PMID: 23603789] 
Functional Description
 NMDA receptor subtype of glutamate-gated ion channels with high calcium permeability and voltage-dependent sensitivity to magnesium. Mediated by glycine. 
Sequence Annotation
 MOTIF 1334 1336 PDZ-binding (By similarity).
 MOD_RES 1326 1326 Phosphoserine (By similarity).
 CARBOHYD 92 92 N-linked (GlcNAc...) (Potential).
 CARBOHYD 352 352 N-linked (GlcNAc...) (Potential).
 CARBOHYD 366 366 N-linked (GlcNAc...) (Potential).
 CARBOHYD 467 467 N-linked (GlcNAc...) (Potential).
 CARBOHYD 569 569 N-linked (GlcNAc...) (Potential).
 DISULFID 455 483 By similarity.
 DISULFID 462 484 By similarity.
 DISULFID 773 828 By similarity.  
Keyword
 Calcium; Cell junction; Cell membrane; Complete proteome; Disulfide bond; Glycoprotein; Ion channel; Ion transport; Ligand-gated ion channel; Magnesium; Membrane; Phosphoprotein; Polymorphism; Postsynaptic cell membrane; Receptor; Reference proteome; Signal; Synapse; Transmembrane; Transmembrane helix; Transport. 
Sequence Source
 UniProt (SWISSPROT/TrEMBL); GenBank; EMBL 
Protein Length
 1336 AA 
Protein Sequence
MRGAGGPRGP RGPAKMLLLL ALACASPFPE EAPGPGGAGG PGGGLGGARP LNVALVFSGP 60
AYAAEAARLG PAVAAAVRSP GLDVRPVALV LNGSDPRSLV LQLCDLLSGL RVHGVVFEDD 120
SRAPAVAPIL DFLSAQTSLP IVAVHGGAAL VLTPKEKGST FLQLGSSTEQ QLQVIFEVLE 180
EYDWTSFVAV TTRAPGHRAF LSYIEVLTDG SLVGWEHRGA LTLDPGAGEA VLSAQLRSVS 240
AQIRLLFCAR EEAEPVFRAA EEAGLTGSGY VWFMVGPQLA GGGGSGAPGE PPLLPGGAPL 300
PAGLFAVRSA GWRDDLARRV AAGVAVVARG AQALLRDYGF LPELGHDCRA QNRTHRGESL 360
HRYFMNITWD NRDYSFNEDG FLVNPSLVVI SLTRDRTWEV VGSWEQQTLR LKYPLWSRYG 420
RFLQPVDDTQ HLTVATLEER PFVIVEPADP ISGTCIRDSV PCRSQLNRTH SPPPDAPRPE 480
KRCCKGFCID ILKRLAHTIG FSYDLYLVTN GKHGKKIDGV WNGMIGEVFY QRADMAIGSL 540
TINEERSEIV DFSVPFVETG ISVMVARSNG TVSPSAFLEP YSPAVWVMMF VMCLTVVAVT 600
VFIFEYLSPV GYNRSLATGK RPGGSTFTIG KSIWLLWALV FNNSVPVENP RGTTSKIMVL 660
VWAFFAVIFL ASYTANLAAF MIQEEYVDTV SGLSDRKFQR PQEQYPPLKF GTVPNGSTEK 720
NIRSNYPDMH SYMVRYNQPR VEEALTQLKA GKLDAFIYDA AVLNYMARKD EGCKLVTIGS 780
GKVFATTGYG IALHKGSRWK RPIDLALLQF LGDDEIEMLE RLWLSGICHN DKIEVMSSKL 840
DIDNMAGVFY MLLVAMGLSL LVFAWEHLVY WRLRHCLGPT HRMDFLLAFS RGMYSCCSAE 900
AAPPPAKPPP PPQPLPSPAY PAPRPAPGPA PFVPRERASV DRWRRTKGAG PPGGAGLADG 960
FHRYYGPIEP QGLGLGLGEA RAAPRGAAGR PLSPPAAQPP QKPPPSYFAI VRDKEPAEPP 1020
AGAFPGFPSP PAPPAAAATA VGPPLCRLAF EDESPPAPAR WPRSDPESQP LLGPGAGGAG 1080
GTGGAGGGAP AAPPPCRAAP PPCPYLDLEP SPSDSEDSES LGGASLGGLE PWWFADFPYP 1140
YAERLGPPPG RYWSVDKLGG WRAGSWDYLP PRSGPAAWHC RHCASLELLP PPRHLSCSHD 1200
GLDGGWWAPP PPPWAAGPLP RRRARCGCPR SHPHRPRASH RTPAAAAPHH HRHRRAAGGW 1260
DLPPPAPTSR SLEDLSSCPR AAPARRLTGP SRHARRCPHA AHWGPPLPTA SHRRHRGGDL 1320
GTRRGSAHFS SLESEV 1336 
Gene Ontology
 GO:0030054; C:cell junction; IEA:UniProtKB-KW.
 GO:0016021; C:integral to membrane; IEA:UniProtKB-KW.
 GO:0030288; C:outer membrane-bounded periplasmic space; IEA:InterPro.
 GO:0005886; C:plasma membrane; TAS:Reactome.
 GO:0045211; C:postsynaptic membrane; IEA:UniProtKB-SubCell.
 GO:0005234; F:extracellular-glutamate-gated ion channel activity; IEA:InterPro.
 GO:0004972; F:N-methyl-D-aspartate selective glutamate receptor activity; IEA:Compara.
 GO:0008344; P:adult locomotory behavior; IEA:Compara.
 GO:0035235; P:ionotropic glutamate receptor signaling pathway; IEA:GOC.
 GO:0051930; P:regulation of sensory perception of pain; IEA:Compara.
 GO:0001964; P:startle response; IEA:Compara.
 GO:0007268; P:synaptic transmission; TAS:Reactome. 
Interpro
 IPR001828; ANF_lig-bd_rcpt.
 IPR019594; Glu_rcpt_Glu/Gly-bd.
 IPR001320; Iontro_glu_rcpt.
 IPR001508; NMDA_rcpt.
 IPR001638; SBP_bac_3. 
Pfam
 PF01094; ANF_receptor
 PF00060; Lig_chan
 PF00497; SBP_bac_3 
SMART
 SM00918; Lig_chan-Glu_bd
 SM00079; PBPe 
PROSITE
  
PRINTS
 PR00177; NMDARECEPTOR.