UniProt Accession

 SPT8_YEAST; P38915; D6VY57 

Genbank Protein ID

 M94955; X94607; Z73227; BK006945 

Genbank Nucleotide ID

 AAA53585.1; CAA64302.1; CAA97585.1; DAA09373.1 

Protein Name

 Transcription factor SPT8 

Protein Synonyms/Alias

  

Gene Name

 SPT8 

Gene Synonyms/Alias

 YLR055C; L2144 

Created Date

 July 27, 2013 

Organism

 Saccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast) 

NCBI Taxa ID

 559292 

Lysine Modification

Position	Peptide	Type	References
114	DSGDAVTKIGSEDVK	acetylation	[1]
121	KIGSEDVKLSDVDGG	ubiquitination	[2]

Reference

 [1] Proteome-wide analysis of lysine acetylation suggests its broad regulatory scope in Saccharomyces cerevisiae.
 Henriksen P, Wagner SA, Weinert BT, Sharma S, Bacinskaja G, Rehman M, Juffer AH, Walther TC, Lisby M, Choudhary C.
 Mol Cell Proteomics. 2012 Nov;11(11):1510-22. [PMID: 22865919]
 [2] Global analysis of phosphorylation and ubiquitylation cross-talk in protein degradation.
 Swaney DL, Beltrao P, Starita L, Guo A, Rush J, Fields S, Krogan NJ, Villén J.
 Nat Methods. 2013 Jul;10(7):676-82. [PMID: 23749301] 

Functional Description

 Required, directly or indirectly, for TATA-binding protein function at particular promoters. May promote a functional interaction between SPT3 and TATA-binding protein. Functions as a component of the transcription regulatory histone acetylation (HAT) complex SAGA. SAGA is involved in RNA polymerase II- dependent transcriptional regulation of approximately 10% of yeast genes. At the promoters, SAGA is required for recruitment of the basal transcription machinery. It influences RNA polymerase II transcriptional activity through different activities such as TBP interaction (SPT3, SPT8 and SPT20) and promoter selectivity, interaction with transcription activators (GCN5, ADA2, ADA3 and TRA1), and chromatin modification through histone acetylation (GCN5) and deubiquitination (UBP8). SAGA acetylates nucleosomal histone H3 to some extent (to form H3K9ac, H3K14ac, H3K18ac and H3K23ac). SAGA interacts with DNA via upstream activating sequences (UASs). 

Sequence Annotation

 REPEAT 173 212 WD 1.
 REPEAT 305 346 WD 2.
 REPEAT 415 454 WD 3.
 REPEAT 506 544 WD 4.
 REPEAT 560 600 WD 5.
 MOD_RES 108 108 Phosphoserine.
 MOD_RES 123 123 Phosphoserine.
 MOD_RES 131 131 Phosphoserine.
 MOD_RES 451 451 Phosphoserine.  

Keyword

 Complete proteome; Nucleus; Phosphoprotein; Reference proteome; Repeat; Transcription; Transcription regulation; WD repeat. 

Sequence Source

 UniProt (SWISSPROT/TrEMBL); GenBank; EMBL 

Protein Length

 602 AA 

Protein Sequence

Gene Ontology

 GO:0000124; C:SAGA complex; IDA:SGD.
 GO:0017025; F:TBP-class protein binding; IDA:SGD.
 GO:0003712; F:transcription cofactor activity; IMP:SGD.
 GO:0016573; P:histone acetylation; IDA:SGD.
 GO:0000122; P:negative regulation of transcription from RNA polymerase II promoter; IDA:SGD.
 GO:0045944; P:positive regulation of transcription from RNA polymerase II promoter; IMP:SGD.
 GO:0006351; P:transcription, DNA-dependent; IEA:UniProtKB-KW. 

Interpro

 IPR015943; WD40/YVTN_repeat-like_dom.
 IPR001680; WD40_repeat.
 IPR017986; WD40_repeat_dom. 

Pfam

 PF00400; WD40 

SMART

 SM00320; WD40 

PROSITE

 PS00678; WD_REPEATS_1
 PS50082; WD_REPEATS_2
 PS50294; WD_REPEATS_REGION 

PRINTS