UniProt Accession

 DPOLB_MOUSE; Q8K409; Q3UAB6; Q922Z7 

Genbank Protein ID

 AF513911; AK077127; AK146745; AK151436; BC006681; BC060998 

Genbank Nucleotide ID

 AAM49616.1; BAC36630.1; BAE27405.1; BAE30399.1; AAH06681.1; AAH60998.1 

Protein Name

 DNA polymerase beta 

Protein Synonyms/Alias

  

Gene Name

 Polb 

Gene Synonyms/Alias

  

Created Date

 July 27, 2013 

Organism

 Mus musculus (Mouse) 

NCBI Taxa ID

 10090 

Lysine Modification

Position	Peptide	Type	References
72	VGTKIAEKIDEFLAT	acetylation	[1, 2, 3]
81	DEFLATGKLRKLEKI	acetylation	[3]

Reference

 [1] Quantitative acetylome analysis reveals the roles of SIRT1 in regulating diverse substrates and cellular pathways.
 Chen Y, Zhao W, Yang JS, Cheng Z, Luo H, Lu Z, Tan M, Gu W, Zhao Y.
 Mol Cell Proteomics. 2012 Oct;11(10):1048-62. [PMID: 22826441]
 [2] Proteomic investigations of lysine acetylation identify diverse substrates of mitochondrial deacetylase sirt3.
 Sol EM, Wagner SA, Weinert BT, Kumar A, Kim HS, Deng CX, Choudhary C.
 PLoS One. 2012;7(12):e50545. [PMID: 23236377]
 [3] SIRT5-Mediated Lysine Desuccinylation Impacts Diverse Metabolic Pathways.
 Park J, Chen Y, Tishkoff DX, Peng C, Tan M, Dai L, Xie Z, Zhang Y, Zwaans BM, Skinner ME, Lombard DB, Zhao Y.
 Mol Cell. 2013 Jun 27;50(6):919-30. [PMID: 23806337] 

Functional Description

 Repair polymerase that plays a key role in base-excision repair. Has 5'-deoxyribose-5-phosphate lyase (dRP lyase) activity that removes the 5' sugar phosphate and also acts as a DNA polymerase that adds one nucleotide to the 3' end of the arising single-nucleotide gap. Conducts 'gap-filling' DNA synthesis in a stepwise distributive fashion rather than in a processive fashion as for other DNA polymerases (By similarity). 

Sequence Annotation

 REGION 183 192 DNA binding (By similarity).
 ACT_SITE 72 72 Schiff-base intermediate with DNA (By
 METAL 101 101 Sodium; via carbonyl oxygen (By
 METAL 103 103 Sodium; via carbonyl oxygen (By
 METAL 106 106 Sodium; via carbonyl oxygen (By
 METAL 190 190 Magnesium 1 (By similarity).
 METAL 190 190 Magnesium 2 (By similarity).
 METAL 192 192 Magnesium 1 (By similarity).
 METAL 192 192 Magnesium 2 (By similarity).
 METAL 256 256 Magnesium 2 (By similarity).
 MOD_RES 83 83 Omega-N-methylarginine; by PRMT6 (By
 MOD_RES 152 152 Omega-N-methylarginine; by PRMT6 (By
 CROSSLNK 41 41 Glycyl lysine isopeptide (Lys-Gly)
 CROSSLNK 61 61 Glycyl lysine isopeptide (Lys-Gly)
 CROSSLNK 81 81 Glycyl lysine isopeptide (Lys-Gly)  

Keyword

 Complete proteome; Cytoplasm; DNA damage; DNA repair; DNA replication; DNA synthesis; DNA-binding; DNA-directed DNA polymerase; Isopeptide bond; Lyase; Magnesium; Metal-binding; Methylation; Nucleotidyltransferase; Nucleus; Reference proteome; Sodium; Transferase; Ubl conjugation. 

Sequence Source

 UniProt (SWISSPROT/TrEMBL); GenBank; EMBL 

Protein Length

 335 AA 

Protein Sequence

Gene Ontology

 GO:0005737; C:cytoplasm; ISS:UniProtKB.
 GO:0005876; C:spindle microtubule; ISO:MGI.
 GO:0000795; C:synaptonemal complex; TAS:MGI.
 GO:0003684; F:damaged DNA binding; IEA:Compara.
 GO:0003887; F:DNA-directed DNA polymerase activity; ISS:UniProtKB.
 GO:0016829; F:lyase activity; ISS:UniProtKB.
 GO:0046872; F:metal ion binding; IEA:UniProtKB-KW.
 GO:0008017; F:microtubule binding; ISO:MGI.
 GO:0006287; P:base-excision repair, gap-filling; IMP:MGI.
 GO:0008219; P:cell death; IMP:MGI.
 GO:0006260; P:DNA replication; IEA:UniProtKB-KW.
 GO:0043066; P:negative regulation of apoptotic process; IMP:MGI.
 GO:0006290; P:pyrimidine dimer repair; IEA:Compara.
 GO:0045471; P:response to ethanol; IEA:Compara. 

Interpro

 IPR002054; DNA-dir_DNA_pol_X.
 IPR019843; DNA_pol-X_BS.
 IPR010996; DNA_pol_b-like_N.
 IPR018944; DNA_pol_lambd_fingers_domain.
 IPR022312; DNA_pol_X.
 IPR002008; DNA_pol_X_beta-like.
 IPR003583; Hlx-hairpin-Hlx_DNA-bd_motif.
 IPR027420; PolB_N. 

Pfam

 PF10391; DNA_pol_lambd_f 

SMART

 SM00278; HhH1
 SM00483; POLXc 

PROSITE

 PS00522; DNA_POLYMERASE_X 

PRINTS

 PR00869; DNAPOLX.
 PR00870; DNAPOLXBETA.