CPLM 1.0 - Compendium of Protein Lysine Modification
TagContent
CPLM ID CPLM-000449
UniProt Accession
Genbank Protein ID
Genbank Nucleotide ID
Protein Name
 Importin-8 
Protein Synonyms/Alias
 Imp8; Ran-binding protein 8; RanBP8 
Gene Name
 IPO8 
Gene Synonyms/Alias
 RANBP8 
Created Date
 July 27, 2013 
Organism
 Homo sapiens (Human) 
NCBI Taxa ID
 9606 
Lysine Modification
Position
Peptide
Type
References
11NRIIQALKGTIDPKLubiquitination[1]
31NELNQSYKIINFAPSubiquitination[1, 2]
380PYEYIRMKFDIFEDYubiquitination[1]
504NAVELAKKSLIEDKEubiquitination[1]
510KKSLIEDKEMPVKVEubiquitination[3]
1009RTVAEAKKKIEQQGGubiquitination[1]
1010TVAEAKKKIEQQGGFubiquitination[1]
Reference
 [1] Refined preparation and use of anti-diglycine remnant (K-ε-GG) antibody enables routine quantification of 10,000s of ubiquitination sites in single proteomics experiments.
 Udeshi ND, Svinkina T, Mertins P, Kuhn E, Mani DR, Qiao JW, Carr SA.
 Mol Cell Proteomics. 2013 Mar;12(3):825-31. [PMID: 23266961]
 [2] Systematic and quantitative assessment of the ubiquitin-modified proteome.
 Kim W, Bennett EJ, Huttlin EL, Guo A, Li J, Possemato A, Sowa ME, Rad R, Rush J, Comb MJ, Harper JW, Gygi SP.
 Mol Cell. 2011 Oct 21;44(2):325-40. [PMID: 21906983]
 [3] A proteome-wide, quantitative survey of in vivo ubiquitylation sites reveals widespread regulatory roles.
 Wagner SA, Beli P, Weinert BT, Nielsen ML, Cox J, Mann M, Choudhary C.
 Mol Cell Proteomics. 2011 Oct;10(10):M111.013284. [PMID: 21890473] 
Functional Description
 Seems to function in nuclear protein import, either by acting as autonomous nuclear transport receptor or as an adapter- like protein in association with the importin-beta subunit KPNB1. Acting autonomously, is thought to serve itself as receptor for nuclear localization signals (NLS) and to promote translocation of import substrates through the nuclear pore complex (NPC) by an energy requiring, Ran-dependent mechanism. At the nucleoplasmic side of the NPC, Ran binds to importin, the importin/substrate complex dissociates and importin is re-exported from the nucleus to the cytoplasm where GTP hydrolysis releases Ran. The directionality of nuclear import is thought to be conferred by an asymmetric distribution of the GTP- and GDP-bound forms of Ran between the cytoplasm and nucleus. In vitro mediates the nuclear import of SRP19. 
Sequence Annotation
 DOMAIN 22 102 Importin N-terminal.
 MOD_RES 902 902 Phosphoserine.
 MOD_RES 903 903 Phosphoserine.  
Keyword
 Alternative splicing; Complete proteome; Cytoplasm; Nucleus; Phosphoprotein; Polymorphism; Protein transport; Reference proteome; Transport. 
Sequence Source
 UniProt (SWISSPROT/TrEMBL); GenBank; EMBL 
Protein Length
 1037 AA 
Protein Sequence
MDLNRIIQAL KGTIDPKLRI AAENELNQSY KIINFAPSLL RIIVSDHVEF PVRQAAAIYL 60
KNMVTQYWPD REPPPGEAIF PFNIHENDRQ QIRDNIVEGI IRSPDLVRVQ LTMCLRAIIK 120
HDFPGHWPGV VDKIDYYLQS QSSASWLGSL LCLYQLVKTY EYKKAEEREP LIIAMQIFLP 180
RIQQQIVQLL PDSSYYSVLL QKQILKIFYA LVQYALPLQL VNNQTMTTWM EIFRTIIDRT 240
VPPETLHIDE DDRPELVWWK CKKWALHIVA RLFERYGSPG NVTKEYFEFS EFFLKTYAVG 300
IQQVLLKILD QYRQKEYVAP RVLQQAFNYL NQGVVHSITW KQMKPHIQNI SEDVIFSVMC 360
YKDEDEELWQ EDPYEYIRMK FDIFEDYASP TTAAQTLLYT AAKKRKEVLP KMMAFCYQIL 420
TDPNFDPRKK DGALHVIGSL AEILLKKSLF KDQMELFLQN HVFPLLLSNL GYLRARSCWV 480
LHAFSSLKFH NELNLRNAVE LAKKSLIEDK EMPVKVEAAL ALQSLISNQI QAKEYMKPHV 540
RPIMQELLHI VRETENDDVT NVIQKMICEY SQEVASIAVD MTQHLAEIFG KVLQSDEYEE 600
VEDKTVMAMG ILHTIDTILT VVEDHKEITQ QLENICLRII DLVLQKHVIE FYEEILSLAY 660
SLTCHSISPQ MWQLLGILYE VFQQDCFEYF TDMMPLLHNY VTIDTDTLLS NAKHLEILFT 720
MCRKVLCGDA GEDAECHAAK LLEVIILQCK GRGIDQCIPL FVQLVLERLT RGVKTSELRT 780
MCLQVAIAAL YYNPDLLLHT LERIQLPHNP GPITVQFINQ WMNDTDCFLG HHDRKMCIIG 840
LSILLELQNR PPAVDAVVGQ IVPSILFLFL GLKQVCATRQ LVNREDRSKA EKADMEENEE 900
ISSDEEETNV TAQAMQSNNG RGEDEEEEDD DWDEEVLEET ALEGFSTPLD LDNSVDEYQF 960
FTQALITVQS RDAAWYQLLM APLSEDQRTA LQEVYTLAEH RRTVAEAKKK IEQQGGFTFE 1020
NKGVLSAFNF GTVPSNN 1037 
Gene Ontology
 GO:0005737; C:cytoplasm; IEA:UniProtKB-SubCell.
 GO:0005643; C:nuclear pore; IEA:InterPro.
 GO:0008565; F:protein transporter activity; IEA:InterPro.
 GO:0008536; F:Ran GTPase binding; TAS:ProtInc.
 GO:0006886; P:intracellular protein transport; IEA:InterPro.
 GO:0007165; P:signal transduction; TAS:ProtInc. 
Interpro
 IPR011989; ARM-like.
 IPR016024; ARM-type_fold.
 IPR013713; Exportin/Importin_Cse1-like.
 IPR001494; Importin-beta_N. 
Pfam
 PF08506; Cse1
 PF03810; IBN_N 
SMART
 SM00913; IBN_N 
PROSITE
 PS50166; IMPORTIN_B_NT 
PRINTS