CPLM 1.0 - Compendium of Protein Lysine Modification
TagContent
CPLM ID CPLM-030780
UniProt Accession
Genbank Protein ID
Genbank Nucleotide ID
Protein Name
 Pumilio homolog 1 
Protein Synonyms/Alias
 cDNA FLJ56067, highly similar to Pumilio homolog 1 
Gene Name
 PUM1 
Gene Synonyms/Alias
  
Created Date
 July 27, 2013 
Organism
 Homo sapiens (Human) 
NCBI Taxa ID
 9606 
Lysine Modification
Position
Peptide
Type
References
143GGGYNNSKHRWPTGDubiquitination[1]
210GDKLGDLKEEGDVMDubiquitination[2]
659TGSSDLYKRTSSSLTubiquitination[1, 2, 3, 4]
735AAPGAEAKYRSASSAacetylation[5]
735AAPGAEAKYRSASSAubiquitination[1, 2, 3, 4, 6, 7]
810GSRFIQLKLERATPAubiquitination[1, 4]
855EFGSLEQKLALAERIubiquitination[2]
881YGCRVIQKALEFIPSubiquitination[1]
904ELDGHVLKCVKDQNGubiquitination[1, 4]
907GHVLKCVKDQNGNHVubiquitination[4]
917NGNHVVQKCIECVQPubiquitination[1, 4]
1017VLVLSQHKFASNVVEubiquitination[1]
1025FASNVVEKCVTHASRubiquitination[1, 4]
1068YANYVVQKMIDVAEPubiquitination[1, 2]
1104YGKHILAKLEKYYMKacetylation[5]
1104YGKHILAKLEKYYMKubiquitination[1]
1107HILAKLEKYYMKNGVubiquitination[1]
Reference
 [1] Refined preparation and use of anti-diglycine remnant (K-ε-GG) antibody enables routine quantification of 10,000s of ubiquitination sites in single proteomics experiments.
 Udeshi ND, Svinkina T, Mertins P, Kuhn E, Mani DR, Qiao JW, Carr SA.
 Mol Cell Proteomics. 2013 Mar;12(3):825-31. [PMID: 23266961]
 [2] Systematic and quantitative assessment of the ubiquitin-modified proteome.
 Kim W, Bennett EJ, Huttlin EL, Guo A, Li J, Possemato A, Sowa ME, Rad R, Rush J, Comb MJ, Harper JW, Gygi SP.
 Mol Cell. 2011 Oct 21;44(2):325-40. [PMID: 21906983]
 [3] A proteome-wide, quantitative survey of in vivo ubiquitylation sites reveals widespread regulatory roles.
 Wagner SA, Beli P, Weinert BT, Nielsen ML, Cox J, Mann M, Choudhary C.
 Mol Cell Proteomics. 2011 Oct;10(10):M111.013284. [PMID: 21890473]
 [4] Integrated proteomic analysis of post-translational modifications by serial enrichment.
 Mertins P, Qiao JW, Patel J, Udeshi ND, Clauser KR, Mani DR, Burgess MW, Gillette MA, Jaffe JD, Carr SA.
 Nat Methods. 2013 Jul;10(7):634-7. [PMID: 23749302]
 [5] Proteomic investigations of lysine acetylation identify diverse substrates of mitochondrial deacetylase sirt3.
 Sol EM, Wagner SA, Weinert BT, Kumar A, Kim HS, Deng CX, Choudhary C.
 PLoS One. 2012;7(12):e50545. [PMID: 23236377]
 [6] Global identification of modular cullin-RING ligase substrates.
 Emanuele MJ, Elia AE, Xu Q, Thoma CR, Izhar L, Leng Y, Guo A, Chen YN, Rush J, Hsu PW, Yen HC, Elledge SJ.
 Cell. 2011 Oct 14;147(2):459-74. [PMID: 21963094]
 [7] Methods for quantification of in vivo changes in protein ubiquitination following proteasome and deubiquitinase inhibition.
 Udeshi ND, Mani DR, Eisenhaure T, Mertins P, Jaffe JD, Clauser KR, Hacohen N, Carr SA.
 Mol Cell Proteomics. 2012 May;11(5):148-59. [PMID: 22505724
Functional Description
  
Sequence Annotation
  
Keyword
 Complete proteome; Reference proteome; Repeat. 
Sequence Source
 UniProt (SWISSPROT/TrEMBL); GenBank; EMBL 
Protein Length
 1127 AA 
Protein Sequence
MPLPPPGPGP EPIPGCTAPT QSPVGRHVVG VKGVGGMSVA CVLKRKAVLW QDSFSPHLKH 60
HPQEPANPNM PVVLTSGTGS QAQPQPAANQ ALAAGTHSSP VPGSIGVAGR SQDDAMVDYF 120
FQRQHGEQLG GGGSGGGGYN NSKHRWPTGD NIHAEHQVRS MDELNHDFQA LALEGRAMGE 180
GPRDADSDEN DKGEKKNKGT FDGDKLGDLK EEGDVMDKTN GLPVQNGIDA DVKDFSRTPG 240
NCQNSANEVD LLGPNQNGSE GLAQLTSTNG AKPVEDFSNM ESQSVPLDPM EHVGMEPLQF 300
DYSGTQVPVD SAAATVGLFD YNSQQQLFQR PNALAVQQLT AAQQQQYALA AAHQPHIAGL 360
APAAFVPNPY IISAAPPGTD PYTAGLAAAA TLGPAVVPHQ YYGVTPWGVY PASLFQQQAA 420
AAAAATNSAN QQTTPQAQQG QQQVLRGGAS QRPLTPNQNQ QGQQTDPLVA AAAVNSALAF 480
GQGLAAGMPG YPVLAPAAYY DQTGALVVNA GARNGLGAPV RLVAPAPVII SSSAAQAAVA 540
AAAASANGAA GGLAGTTNGP FRPLGTQQPQ PQPQQQPNNN LASSSFYGNN SLNSNSQSSS 600
LFSQGSAQPA NTSLGFGSSS SLGATLGSAL GGFGTAVANS NTGSGSRRDS LTGSSDLYKR 660
TSSSLTPIGH SFYNGLSFSS SPGPVGMPLP SQGPGHSQTP PPSLSSHGSS SSLNLGGLTN 720
GSGRYISAAP GAEAKYRSAS SASSLFSPSS TLFSSSRLRY GMSDVMPSGR SRLLEDFRNN 780
RYPNLQLREI AGHIMEFSQD QHGSRFIQLK LERATPAERQ LVFNEILQAA YQLMVDVFGN 840
YVIQKFFEFG SLEQKLALAE RIRGHVLSLA LQMYGCRVIQ KALEFIPSDQ QNEMVRELDG 900
HVLKCVKDQN GNHVVQKCIE CVQPQSLQFI IDAFKGQVFA LSTHPYGCRV IQRILEHCLP 960
DQTLPILEEL HQHTEQLVQD QYGNYVIQHV LEHGRPEDKS KIVAEIRGNV LVLSQHKFAS 1020
NVVEKCVTHA SRTERAVLID EVCTMNDGPH SALYTMMKDQ YANYVVQKMI DVAEPGQRKI 1080
VMHKIRPHIA TLRKYTYGKH ILAKLEKYYM KNGVDLGPIC GPPNGII 1127 
Gene Ontology
 GO:0003723; F:RNA binding; IEA:InterPro. 
Interpro
 IPR011989; ARM-like.
 IPR016024; ARM-type_fold.
 IPR001313; Pumilio_RNA-bd_rpt. 
Pfam
 PF00806; PUF 
SMART
 SM00025; Pumilio 
PROSITE
 PS50302; PUM
 PS50303; PUM_HD 
PRINTS