CPLM 1.0 - Compendium of Protein Lysine Modification
TagContent
CPLM ID CPLM-021548
UniProt Accession
Genbank Protein ID
Genbank Nucleotide ID
Protein Name
 Protein argonaute-3 
Protein Synonyms/Alias
 Argonaute3; hAgo3; Argonaute RISC catalytic component 3; Eukaryotic translation initiation factor 2C 3; eIF-2C 3; eIF2C 3 
Gene Name
 AGO3 
Gene Synonyms/Alias
 EIF2C3 
Created Date
 July 27, 2013 
Organism
 Homo sapiens (Human) 
NCBI Taxa ID
 9606 
Lysine Modification
Position
Peptide
Type
References
174LRHLPSMKYTPVGRSubiquitination[1]
Reference
 [1] Refined preparation and use of anti-diglycine remnant (K-ε-GG) antibody enables routine quantification of 10,000s of ubiquitination sites in single proteomics experiments.
 Udeshi ND, Svinkina T, Mertins P, Kuhn E, Mani DR, Qiao JW, Carr SA.
 Mol Cell Proteomics. 2013 Mar;12(3):825-31. [PMID: 23266961] 
Functional Description
 Required for RNA-mediated gene silencing (RNAi). Binds to short RNAs such as microRNAs (miRNAs) and represses the translation of mRNAs which are complementary to them. Lacks endonuclease activity and does not appear to cleave target mRNAs. Proposed to be involved in stabilization of small RNA derivates (riRNA) derived from processed RNA polymerase III-transcribed Alu repeats containing a DR2 retinoic acid response element (RARE) in stem cells and in the subsequent riRNA-dependent degradation of a subset of RNA polymerase II-transcribed coding mRNAs by recruiting a mRNA decapping complex involving EDC4. 
Sequence Annotation
 DOMAIN 236 349 PAZ.
 DOMAIN 518 819 Piwi.  
Keyword
 Alternative splicing; Complete proteome; Cytoplasm; Reference proteome; Ribonucleoprotein; RNA-binding; RNA-mediated gene silencing; Translation regulation. 
Sequence Source
 UniProt (SWISSPROT/TrEMBL); GenBank; EMBL 
Protein Length
 860 AA 
Protein Sequence
MEIGSAGPAG AQPLLMVPRR PGYGTMGKPI KLLANCFQVE IPKIDVYLYE VDIKPDKCPR 60
RVNREVVDSM VQHFKVTIFG DRRPVYDGKR SLYTANPLPV ATTGVDLDVT LPGEGGKDRP 120
FKVSIKFVSR VSWHLLHEVL TGRTLPEPLE LDKPISTNPV HAVDVVLRHL PSMKYTPVGR 180
SFFSAPEGYD HPLGGGREVW FGFHQSVRPA MWKMMLNIDV SATAFYKAQP VIQFMCEVLD 240
IHNIDEQPRP LTDSHRVKFT KEIKGLKVEV THCGTMRRKY RVCNVTRRPA SHQTFPLQLE 300
NGQTVERTVA QYFREKYTLQ LKYPHLPCLQ VGQEQKHTYL PLEVCNIVAG QRCIKKLTDN 360
QTSTMIKATA RSAPDRQEEI SRLVRSANYE TDPFVQEFQF KVRDEMAHVT GRVLPAPMLQ 420
YGGRNRTVAT PSHGVWDMRG KQFHTGVEIK MWAIACFATQ RQCREEILKG FTDQLRKISK 480
DAGMPIQGQP CFCKYAQGAD SVEPMFRHLK NTYSGLQLII VILPGKTPVY AEVKRVGDTL 540
LGMATQCVQV KNVIKTSPQT LSNLCLKINV KLGGINNILV PHQRPSVFQQ PVIFLGADVT 600
HPPAGDGKKP SIAAVVGSMD AHPSRYCATV RVQRPRQEII QDLASMVREL LIQFYKSTRF 660
KPTRIIFYRD GVSEGQFRQV LYYELLAIRE ACISLEKDYQ PGITYIVVQK RHHTRLFCAD 720
RTERVGRSGN IPAGTTVDTD ITHPYEFDFY LCSHAGIQGT SRPSHYHVLW DDNCFTADEL 780
QLLTYQLCHT YVRCTRSVSI PAPAYYAHLV AFRARYHLVD KEHDSAEGSH VSGQSNGRDP 840
QALAKAVQIH QDTLRTMYFA 860 
Gene Ontology
 GO:0000932; C:cytoplasmic mRNA processing body; IEA:UniProtKB-SubCell.
 GO:0005829; C:cytosol; TAS:Reactome.
 GO:0035068; C:micro-ribonucleoprotein complex; IEA:Compara.
 GO:0003723; F:RNA binding; IEA:UniProtKB-KW.
 GO:0007173; P:epidermal growth factor receptor signaling pathway; TAS:Reactome.
 GO:0008543; P:fibroblast growth factor receptor signaling pathway; TAS:Reactome.
 GO:0010467; P:gene expression; TAS:Reactome.
 GO:0045087; P:innate immune response; TAS:Reactome.
 GO:0006402; P:mRNA catabolic process; IDA:UniProtKB.
 GO:0035278; P:negative regulation of translation involved in gene silencing by miRNA; IDA:UniProtKB.
 GO:0048011; P:neurotrophin TRK receptor signaling pathway; TAS:Reactome.
 GO:0007219; P:Notch signaling pathway; TAS:Reactome.
 GO:0048015; P:phosphatidylinositol-mediated signaling; TAS:Reactome.
 GO:0072091; P:regulation of stem cell proliferation; IMP:UniProtKB. 
Interpro
 IPR014811; DUF1785.
 IPR003100; PAZ.
 IPR003165; Piwi.
 IPR012337; RNaseH-like_dom. 
Pfam
 PF08699; DUF1785
 PF02170; PAZ
 PF02171; Piwi 
SMART
 SM00949; PAZ
 SM00950; Piwi 
PROSITE
 PS50821; PAZ
 PS50822; PIWI 
PRINTS