CPLM-011173

Genbank Nucleotide ID

UBX domain-containing protein 2

Protein Synonyms/Alias

Secretion lowering protein 1

SEL1; YML013W; YM9571.05

Saccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast)

Position	Peptide	Type	References
205	ILGDTKDKDAFCELK	ubiquitination	[1]
215	FCELKSFKPDISFNE	ubiquitination	[1]
228	NEALRIAKEEFKFML	ubiquitination	[1]
266	EKILLNKKTLQYLRK	ubiquitination	[1]
314	LIANVANKASHSETL	ubiquitination	[1]
373	RMSREIKKLQEDAYK	ubiquitination	[1]
380	KLQEDAYKKSLEMDR	ubiquitination	[1]
381	LQEDAYKKSLEMDRI	ubiquitination	[1]
430	QPFETTGKQATLQFR	ubiquitination	[1]

[1] Global analysis of phosphorylation and ubiquitylation cross-talk in protein degradation.
Swaney DL, Beltrao P, Starita L, Guo A, Rush J, Fields S, Krogan NJ, Villén J.
Nat Methods. 2013 Jul;10(7):676-82. [PMID: 23749301]

Functional Description

Integral endoplasmic reticulum membrane protein that coordinates the assembly of the ER-associated protein degradation (ERAD) machinery at the ER membrane. Mediates binding of CDC48 to the E3 ubiquitin ligases SSM4/DOA10 and HRD1, and to ERAD substrates. Component of the DOA10 ubiquitin ligase complex, which is part of the ERAD-C pathway responsible for the rapid degradation of membrane proteins with misfolded cytoplasmic domains. ERAD-C substrates are ubiquitinated through DOA10 in conjunction with the E2 ubiquitin-conjugating enzymes UBC6 and UBC7-CUE1. Also component of the HRD1 ubiquitin ligase complex, which is part of the ERAD-L and ERAD-M pathways responsible for the rapid degradation of soluble lumenal and membrane proteins with misfolded lumenal domains (ERAD-L), or ER-membrane proteins with misfolded transmembrane domains (ERAD-M). ERAD-L substrates are ubiquitinated through HRD1 in conjunction with the E2 ubiquitin-conjugating enzymes UBC1 and UBC7-CUE1. Ubiquitinated substrates are then removed to the cytosol via the action of the UFD1-NPL4-CDC48/p97 (UNC) AAA ATPase complex and targeted to the proteasome.

Complete proteome; Endoplasmic reticulum; Membrane; Reference proteome; Transmembrane; Transmembrane helix; Ubl conjugation pathway.

UniProt (SWISSPROT/TrEMBL); GenBank; EMBL

GO:0000837; C:Doa10p ubiquitin ligase complex; IDA:SGD.
GO:0030176; C:integral to endoplasmic reticulum membrane; IDA:SGD.
GO:0005811; C:lipid particle; IDA:SGD.
GO:0005741; C:mitochondrial outer membrane; IDA:SGD.
GO:0030433; P:ER-associated protein catabolic process; IMP:SGD.
GO:0034389; P:lipid particle organization; IMP:SGD.

IPR009060; UBA-like.
IPR001012; UBX.