UniProt Accession

 H3BR35_HUMAN; H3BR35 

Genbank Protein ID

 AC007216 

Genbank Nucleotide ID

  

Protein Name

 Eukaryotic peptide chain release factor GTP-binding subunit ERF3A 

Protein Synonyms/Alias

  

Gene Name

 GSPT1 

Gene Synonyms/Alias

  

Created Date

 July 27, 2013 

Organism

 Homo sapiens (Human) 

NCBI Taxa ID

 9606 

Lysine Modification

Position	Peptide	Type	References
114	VDKRTLEKYEREAKE	acetylation	[1]
114	VDKRTLEKYEREAKE	ubiquitination	[2]
158	AYFETEKKHFTILDA	ubiquitination	[2]
193	VLVISARKGEFETGF	ubiquitination	[2]
202	EFETGFEKGGQTREH	acetylation	[3]
202	EFETGFEKGGQTREH	ubiquitination	[1, 2, 4]
214	REHAMLAKTAGVKHL	acetylation	[1, 3]
244	NERYEECKEKLVPFL	acetylation	[1]
246	RYEECKEKLVPFLKK	acetylation	[3]
246	RYEECKEKLVPFLKK	ubiquitination	[2]
252	EKLVPFLKKVGFNPK	acetylation	[3]
253	KLVPFLKKVGFNPKK	ubiquitination	[2]
259	KKVGFNPKKDIHFMP	acetylation	[1]
315	IRLPIVDKYKDMGTV	ubiquitination	[5]
326	MGTVVLGKLESGSIC	ubiquitination	[2]
373	ENLKIRLKGIEEEEI	ubiquitination	[2]
424	KTRPRFVKQDQVCIA	acetylation	[3]
424	KTRPRFVKQDQVCIA	ubiquitination	[1, 2, 4, 6]
445	TICLETFKDFPQMGR	ubiquitination	[2]
466	GKTIAIGKVLKLVPE	acetylation	[3]
466	GKTIAIGKVLKLVPE	ubiquitination	[5]
469	IAIGKVLKLVPEKD*	acetylation	[7]
469	IAIGKVLKLVPEKD*	ubiquitination	[2]

Reference

 [1] Integrated proteomic analysis of post-translational modifications by serial enrichment.
 Mertins P, Qiao JW, Patel J, Udeshi ND, Clauser KR, Mani DR, Burgess MW, Gillette MA, Jaffe JD, Carr SA.
 Nat Methods. 2013 Jul;10(7):634-7. [PMID: 23749302]
 [2] Refined preparation and use of anti-diglycine remnant (K-ε-GG) antibody enables routine quantification of 10,000s of ubiquitination sites in single proteomics experiments.
 Udeshi ND, Svinkina T, Mertins P, Kuhn E, Mani DR, Qiao JW, Carr SA.
 Mol Cell Proteomics. 2013 Mar;12(3):825-31. [PMID: 23266961]
 [3] Proteomic investigations of lysine acetylation identify diverse substrates of mitochondrial deacetylase sirt3.
 Sol EM, Wagner SA, Weinert BT, Kumar A, Kim HS, Deng CX, Choudhary C.
 PLoS One. 2012;7(12):e50545. [PMID: 23236377]
 [4] Methods for quantification of in vivo changes in protein ubiquitination following proteasome and deubiquitinase inhibition.
 Udeshi ND, Mani DR, Eisenhaure T, Mertins P, Jaffe JD, Clauser KR, Hacohen N, Carr SA.
 Mol Cell Proteomics. 2012 May;11(5):148-59. [PMID: 22505724]
 [5] A proteome-wide, quantitative survey of in vivo ubiquitylation sites reveals widespread regulatory roles.
 Wagner SA, Beli P, Weinert BT, Nielsen ML, Cox J, Mann M, Choudhary C.
 Mol Cell Proteomics. 2011 Oct;10(10):M111.013284. [PMID: 21890473]
 [6] Systematic and quantitative assessment of the ubiquitin-modified proteome.
 Kim W, Bennett EJ, Huttlin EL, Guo A, Li J, Possemato A, Sowa ME, Rad R, Rush J, Comb MJ, Harper JW, Gygi SP.
 Mol Cell. 2011 Oct 21;44(2):325-40. [PMID: 21906983]
 [7] Proteomic investigations reveal a role for RNA processing factor THRAP3 in the DNA damage response.
 Beli P, Lukashchuk N, Wagner SA, Weinert BT, Olsen JV, Baskcomb L, Mann M, Jackson SP, Choudhary C.
 Mol Cell. 2012 Apr 27;46(2):212-25. [PMID: 22424773] 

Functional Description

  

Sequence Annotation

  

Keyword

 Complete proteome; GTP-binding; Nucleotide-binding; Reference proteome. 

Sequence Source

 UniProt (SWISSPROT/TrEMBL); GenBank; EMBL 

Protein Length

 475 AA 

Protein Sequence

Gene Ontology

 GO:0005525; F:GTP binding; IEA:UniProtKB-KW.
 GO:0003924; F:GTPase activity; IEA:InterPro.
 GO:0006184; P:GTP catabolic process; IEA:GOC. 

Interpro

 IPR000795; EF_GTP-bd_dom.
 IPR027417; P-loop_NTPase.
 IPR009001; Transl_elong_EF1A/Init_IF2_C.
 IPR004161; Transl_elong_EFTu/EF1A_2.
 IPR004160; Transl_elong_EFTu/EF1A_C.
 IPR009000; Transl_elong_init/rib_B-barrel. 

Pfam

 PF00009; GTP_EFTU
 PF03144; GTP_EFTU_D2
 PF03143; GTP_EFTU_D3 

SMART

  

PROSITE

 PS00301; EFACTOR_GTP 

PRINTS

 PR00315; ELONGATNFCT.