UniProt Accession

 GNPI1_HUMAN; P46926; D3DQE7 

Genbank Protein ID

 AF048826; AJ002231; AF029914; AF035809; AF035804; AF035805; AF035806; AF035807; AF035808; D31766; AC005740; CH471062; CH471062; CH471062; CH471062; BC012853; BC020769; BC022322 

Genbank Nucleotide ID

 AAC05123.1; CAA05259.1; AAB84217.1; AAB88748.1; AAB88748.1; AAB88748.1; AAB88748.1; AAB88748.1; AAB88748.1; BAA06544.2; AAC62119.1; EAW61890.1; EAW61891.1; EAW61892.1; EAW61893.1; AAH12853.1; AAH20769.1; AAH22322.1 

Protein Name

 Glucosamine-6-phosphate isomerase 1 

Protein Synonyms/Alias

 Glucosamine-6-phosphate deaminase 1; GNPDA 1; GlcN6P deaminase 1; Oscillin 

Gene Name

 GNPDA1 

Gene Synonyms/Alias

 GNPI; HLN; KIAA0060 

Created Date

 July 27, 2013 

Organism

 Homo sapiens (Human) 

NCBI Taxa ID

 9606 

Lysine Modification

Position	Peptide	Type	References
50	STPLGCYKKLIEYYK	acetylation	[1]
50	STPLGCYKKLIEYYK	ubiquitination	[2, 3]
51	TPLGCYKKLIEYYKN	ubiquitination	[4]
57	KKLIEYYKNGDLSFK	ubiquitination	[4]
64	KNGDLSFKYVKTFNM	acetylation	[1]
67	DLSFKYVKTFNMDEY	ubiquitination	[2, 3, 4, 5, 6]
126	DAFEEKIKAAGGIEL	ubiquitination	[4]
160	LVSRTRVKTLAMDTI	ubiquitination	[4, 6]
253	ELKVKTVKYFKGLML	ubiquitination	[4]
256	VKTVKYFKGLMLVHN	ubiquitination	[2, 3, 4, 6, 7]
264	GLMLVHNKLVDPLYS	ubiquitination	[2, 3, 4, 7]
273	VDPLYSIKEKETEKS	ubiquitination	[5]

Reference

 [1] Lysine acetylation targets protein complexes and co-regulates major cellular functions.
 Choudhary C, Kumar C, Gnad F, Nielsen ML, Rehman M, Walther TC, Olsen JV, Mann M.
 Science. 2009 Aug 14;325(5942):834-40. [PMID: 19608861]
 [2] A proteome-wide, quantitative survey of in vivo ubiquitylation sites reveals widespread regulatory roles.
 Wagner SA, Beli P, Weinert BT, Nielsen ML, Cox J, Mann M, Choudhary C.
 Mol Cell Proteomics. 2011 Oct;10(10):M111.013284. [PMID: 21890473]
 [3] hCKSAAP_UbSite: improved prediction of human ubiquitination sites by exploiting amino acid pattern and properties.
 Chen Z, Zhou Y, Song J, Zhang Z.
 Biochim Biophys Acta. 2013 Aug;1834(8):1461-7. [PMID: 23603789]
 [4] Refined preparation and use of anti-diglycine remnant (K-ε-GG) antibody enables routine quantification of 10,000s of ubiquitination sites in single proteomics experiments.
 Udeshi ND, Svinkina T, Mertins P, Kuhn E, Mani DR, Qiao JW, Carr SA.
 Mol Cell Proteomics. 2013 Mar;12(3):825-31. [PMID: 23266961]
 [5] Systematic and quantitative assessment of the ubiquitin-modified proteome.
 Kim W, Bennett EJ, Huttlin EL, Guo A, Li J, Possemato A, Sowa ME, Rad R, Rush J, Comb MJ, Harper JW, Gygi SP.
 Mol Cell. 2011 Oct 21;44(2):325-40. [PMID: 21906983]
 [6] Systems-wide analysis of ubiquitylation dynamics reveals a key role for PAF15 ubiquitylation in DNA-damage bypass.
 Povlsen LK, Beli P, Wagner SA, Poulsen SL, Sylvestersen KB, Poulsen JW, Nielsen ML, Bekker-Jensen S, Mailand N, Choudhary C.
 Nat Cell Biol. 2012 Oct;14(10):1089-98. [PMID: 23000965]
 [7] Landscape of the PARKIN-dependent ubiquitylome in response to mitochondrial depolarization.
 Sarraf SA, Raman M, Guarani-Pereira V, Sowa ME, Huttlin EL, Gygi SP, Harper JW.
 Nature. 2013 Apr 18;496(7445):372-6. [PMID: 23503661] 

Functional Description

 Seems to trigger calcium oscillations in mammalian eggs. These oscillations serve as the essential trigger for egg activation and early development of the embryo (By similarity). 

Sequence Annotation

 ACT_SITE 72 72 Proton acceptor; for enolization step (By
 ACT_SITE 141 141 For ring-opening step (By similarity).
 ACT_SITE 143 143 Proton acceptor; for ring-opening step
 ACT_SITE 148 148 For ring-opening step (By similarity).
 MOD_RES 64 64 N6-acetyllysine.  

Keyword

 3D-structure; Acetylation; Carbohydrate metabolism; Complete proteome; Cytoplasm; Hydrolase; Reference proteome. 

Sequence Source

 UniProt (SWISSPROT/TrEMBL); GenBank; EMBL 

Protein Length

 289 AA 

Protein Sequence

Gene Ontology

 GO:0005737; C:cytoplasm; ISS:UniProtKB.
 GO:0004342; F:glucosamine-6-phosphate deaminase activity; IMP:UniProtKB.
 GO:0016787; F:hydrolase activity; IEA:UniProtKB-KW.
 GO:0005975; P:carbohydrate metabolic process; IEA:UniProtKB-KW.
 GO:0006091; P:generation of precursor metabolites and energy; IMP:UniProtKB.
 GO:0006043; P:glucosamine catabolic process; IMP:UniProtKB.
 GO:0006044; P:N-acetylglucosamine metabolic process; IEA:InterPro.
 GO:0007338; P:single fertilization; TAS:ProtInc. 

Interpro

 IPR006148; Glc/Gal-6P_isomerase.
 IPR004547; Glucosamine6P_isomerase.
 IPR018321; Glucosamine6P_isomerase_CS. 

Pfam

 PF01182; Glucosamine_iso 

SMART

  

PROSITE

 PS01161; GLC_GALNAC_ISOMERASE 

PRINTS