CPLM 1.0 - Compendium of Protein Lysine Modification
TagContent
CPLM ID CPLM-006653
UniProt Accession
Genbank Protein ID
Genbank Nucleotide ID
Protein Name
 Coproporphyrinogen-III oxidase, aerobic 
Protein Synonyms/Alias
 Coprogen oxidase; Coproporphyrinogenase 
Gene Name
 hemF 
Gene Synonyms/Alias
 b2436; JW2429 
Created Date
 July 27, 2013 
Organism
 Escherichia coli (strain K12) 
NCBI Taxa ID
 83333 
Lysine Modification
Position
Peptide
Type
References
281WEYDYQPKDGSPEAAacetylation[1]
294AALSEFIKVRDWV**acetylation[1]
Reference
 [1] Acetyl-Phosphate Is a Critical Determinant of Lysine Acetylation in E. coli.
 Weinert BT, Iesmantavicius V, Wagner SA, Schölz C, Gummesson B, Beli P, Nyström T, Choudhary C.
 Mol Cell. 2013 Jul 25;51(2):265-72. [PMID: 23830618] 
Functional Description
 Key enzyme in heme biosynthesis. Catalyzes the oxidative decarboxylation of propionic acid side chains of rings A and B of coproporphyrinogen III (By similarity). 
Sequence Annotation
 REGION 48 57 Important for dimerization (By
 REGION 108 110 Substrate binding (By similarity).
 REGION 240 275 Important for dimerization (By
 REGION 258 263 Substrate binding (By similarity).
 ACT_SITE 106 106 Proton donor (By similarity).
 METAL 96 96 Manganese.
 METAL 106 106 Manganese.
 METAL 145 145 Manganese.
 METAL 175 175 Manganese.
 BINDING 92 92 Substrate (By similarity).  
Keyword
 Complete proteome; Cytoplasm; Manganese; Metal-binding; Oxidoreductase; Porphyrin biosynthesis; Reference proteome. 
Sequence Source
 UniProt (SWISSPROT/TrEMBL); GenBank; EMBL 
Protein Length
 299 AA 
Protein Sequence
MKPDAHQVKQ FLLNLQDTIC QQLTAVDGAE FVEDSWQREA GGGGRSRVLR NGGVFEQAGV 60
NFSHVHGEAM PASATAHRPE LAGRSFEAMG VSLVVHPHNP YVPTSHANVR FFIAEKPGAD 120
PVWWFGGGFD LTPFYGFEED AIHWHRTARD LCLPFGEDVY PRYKKWCDEY FYLKHRNEQR 180
GIGGLFFDDL NTPDFDRCFA FMQAVGKGYT DAYLPIVERR KAMAYGERER NFQLYRRGRY 240
VEFNLVWDRG TLFGLQTGGR TESILMSMPP LVRWEYDYQP KDGSPEAALS EFIKVRDWV 299 
Gene Ontology
 GO:0005737; C:cytoplasm; IDA:EcoliWiki.
 GO:0004109; F:coproporphyrinogen oxidase activity; IDA:EcoCyc.
 GO:0030145; F:manganese ion binding; IDA:EcoCyc.
 GO:0042803; F:protein homodimerization activity; ISS:UniProtKB.
 GO:0046906; F:tetrapyrrole binding; IMP:EcoliWiki.
 GO:0019353; P:protoporphyrinogen IX biosynthetic process from glutamate; IGI:EcoCyc. 
Interpro
 IPR001260; Coprogen_oxidase_aer.
 IPR018375; Coprogen_oxidase_CS. 
Pfam
 PF01218; Coprogen_oxidas 
SMART
  
PROSITE
 PS01021; COPROGEN_OXIDASE 
PRINTS
 PR00073; COPRGNOXDASE.