Tag | Content |
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CPLM ID | CPLM-023536 |
UniProt Accession | |
Genbank Protein ID | |
Genbank Nucleotide ID | |
Protein Name | Poly(U)-binding-splicing factor PUF60 |
Protein Synonyms/Alias | 60 kDa poly(U)-binding-splicing factor; RNA-binding protein Siah-BP; Siah-binding protein 1 |
Gene Name | Puf60 |
Gene Synonyms/Alias | Siahbp1 |
Created Date | July 27, 2013 |
Organism | Rattus norvegicus (Rat) |
NCBI Taxa ID | 10116 |
Lysine Modification | Position | Peptide | Type | References |
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459 | ARHMVMQKLLRKQES | acetylation | [1] |
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Reference | [1] Proteomic analysis of lysine acetylation sites in rat tissues reveals organ specificity and subcellular patterns. Lundby A, Lage K, Weinert BT, Bekker-Jensen DB, Secher A, Skovgaard T, Kelstrup CD, Dmytriyev A, Choudhary C, Lundby C, Olsen JV. Cell Rep. 2012 Aug 30;2(2):419-31. [ PMID: 22902405] |
Functional Description | DNA- and RNA-binding protein, involved in several nuclear processes such as pre-mRNA splicing, apoptosis and transcription regulation. In association with FUBP1 regulates MYC transcription at the P2 promoter through the core-TFIIH basal transcription factor. Acts as a transcriptional repressor through the core-TFIIH basal transcription factor. Represses FUBP1-induced transcriptional activation but not basal transcription. Decreases ERCC3 helicase activity. Is also involved in pre-mRNA splicing. Promotes splicing of an intron with weak 3'-splice site and pyrimidine tract in a cooperative manner with U2AF2. Involved in apoptosis induction when overexpressed in HeLa cells. Modulates alternative splicing of several mRNAs. Binds to relaxed DNA of active promoter regions. Binds to the pyrimidine tract and 3'- splice site regions of pre-mRNA; binding is enhanced in presence of U2AF2. Binds to Y5 RNA in association with TROVE2. Binds to poly(U) RNA (By similarity). |
Sequence Annotation | DOMAIN 134 212 RRM 1. DOMAIN 231 309 RRM 2. DOMAIN 467 554 RRM 3; atypical. REGION 1 521 Inhibits homodimerization (By REGION 82 564 Inhibits transcriptional repression, MOD_RES 65 65 Phosphothreonine (By similarity). MOD_RES 117 117 Phosphoserine (By similarity). MOD_RES 256 256 N6-acetyllysine (By similarity). MOD_RES 319 319 Phosphothreonine (By similarity). MOD_RES 459 459 N6-acetyllysine (By similarity). |
Keyword | Acetylation; Alternative splicing; Apoptosis; Complete proteome; DNA-binding; mRNA processing; mRNA splicing; Nucleus; Phosphoprotein; Reference proteome; Repeat; Repressor; Ribonucleoprotein; RNA-binding; Transcription; Transcription regulation. |
Sequence Source | UniProt (SWISSPROT/TrEMBL); GenBank; EMBL |
Protein Length | 564 AA |
Protein Sequence | MATATIALQV NGQQGGGSEP AAAAAAAAAA VVAAGDKWKP PQGTESIKME NGQSTGTKLG 60 LPPLTPEQQE ALQKAKKYAM EQSIKSVLVK QTIAHQQQQL TNLQMAAVTM GFGDPLSPLQ 120 SMAAQRQRAL AIMCRVYVGS IYYELGEDTI RQAFAPFGPI KSIDMSWDSV TMKHKGFAFV 180 EYEVPEAAQL ALEQMNSVML GGRNIKVGRP SNIGQAQPII DQLAEEARAF NRIYVASVHQ 240 DLSDDDIKSV FEAFGKIKSC TLARDPTTGK HKGYGFIEYE KAQSSQDAVS SMNLFDLGGQ 300 YLRVGKAVTP PMPLLTPATP GGLPPAAAVA AAAATAKITA QEAVAGAAVL GTLATPGLVS 360 PALTLAQPLG ALPQAVMAAQ APGVITGVTP ARPPIPVTIP SVGVVNPILA SPPTLGLLEP 420 KKEKEEEELF PESERPEMLS EQEHMSISGS SARHMVMQKL LRKQESTVMV LRNMVDPKDI 480 DDDLEGEVTE ECGKFGAVNR VIIYQEKQGE EEDAEIIVKI FVEFSMASET HKAIQALNGR 540 WFGGRKVVAE VYDQERFDNS DLSA 564 |
Gene Ontology | GO:0019907; C:cyclin-dependent protein kinase activating kinase holoenzyme complex; IEA:Compara. GO:0030529; C:ribonucleoprotein complex; IEA:UniProtKB-KW. GO:0003677; F:DNA binding; IEA:UniProtKB-KW. GO:0000166; F:nucleotide binding; IEA:InterPro. GO:0003723; F:RNA binding; IEA:UniProtKB-KW. GO:0006915; P:apoptotic process; IEA:UniProtKB-KW. GO:0006397; P:mRNA processing; IEA:UniProtKB-KW. GO:0006355; P:regulation of transcription, DNA-dependent; IEA:UniProtKB-KW. GO:0008380; P:RNA splicing; IEA:UniProtKB-KW. GO:0006351; P:transcription, DNA-dependent; IEA:UniProtKB-KW. |
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