CPLM 1.0 - Compendium of Protein Lysine Modification
TagContent
CPLM ID CPLM-012257
UniProt Accession
Genbank Protein ID
Genbank Nucleotide ID
Protein Name
 Probable ATP-dependent RNA helicase DHX34 
Protein Synonyms/Alias
 DEAH box protein 34 
Gene Name
 DHX34 
Gene Synonyms/Alias
 DDX34; KIAA0134 
Created Date
 July 27, 2013 
Organism
 Homo sapiens (Human) 
NCBI Taxa ID
 9606 
Lysine Modification
Position
Peptide
Type
References
27SEEEALEKWDWNCPEubiquitination[1]
59QGSEECQKFWTFFERubiquitination[1]
146HYLDFGQKQAFGRLAubiquitination[1, 2]
353EAEPTTSKSEKLDPRubiquitination[3]
471MSYDPQAKLQRLQEFubiquitination[1]
533DSLVLQMKSMSVGDPubiquitination[1, 2]
801REQLALLKLVLGRGLubiquitination[1]
823DAFNSSRKDSDQIFHubiquitination[1]
985KEVATLSKELLQFTAubiquitination[1]
Reference
 [1] Refined preparation and use of anti-diglycine remnant (K-ε-GG) antibody enables routine quantification of 10,000s of ubiquitination sites in single proteomics experiments.
 Udeshi ND, Svinkina T, Mertins P, Kuhn E, Mani DR, Qiao JW, Carr SA.
 Mol Cell Proteomics. 2013 Mar;12(3):825-31. [PMID: 23266961]
 [2] Global identification of modular cullin-RING ligase substrates.
 Emanuele MJ, Elia AE, Xu Q, Thoma CR, Izhar L, Leng Y, Guo A, Chen YN, Rush J, Hsu PW, Yen HC, Elledge SJ.
 Cell. 2011 Oct 14;147(2):459-74. [PMID: 21963094]
 [3] Systematic and quantitative assessment of the ubiquitin-modified proteome.
 Kim W, Bennett EJ, Huttlin EL, Guo A, Li J, Possemato A, Sowa ME, Rad R, Rush J, Comb MJ, Harper JW, Gygi SP.
 Mol Cell. 2011 Oct 21;44(2):325-40. [PMID: 21906983] 
Functional Description
 Probable ATP-binding RNA helicase. 
Sequence Annotation
 DOMAIN 172 332 Helicase ATP-binding.
 DOMAIN 368 536 Helicase C-terminal.
 NP_BIND 185 192 ATP (By similarity).
 MOTIF 279 282 DEAH box.
 MOD_RES 749 749 Phosphoserine.
 MOD_RES 750 750 Phosphoserine.  
Keyword
 ATP-binding; Complete proteome; Helicase; Hydrolase; Nucleotide-binding; Phosphoprotein; Polymorphism; Reference proteome; RNA-binding. 
Sequence Source
 UniProt (SWISSPROT/TrEMBL); GenBank; EMBL 
Protein Length
 1143 AA 
Protein Sequence
MPPPRTREGR DRRDHHRAPS EEEALEKWDW NCPETRRLLE DAFFREEDYI RQGSEECQKF 60
WTFFERLQRF QNLKTSRKEE KDPGQPKHSI PALADLPRTY DPRYRINLSV LGPATRGSQG 120
LGRHLPAERV AEFRRALLHY LDFGQKQAFG RLAKLQRERA ALPIAQYGNR ILQTLKEHQV 180
VVVAGDTGCG KSTQVPQYLL AAGFSHVACT QPRRIACISL AKRVGFESLS QYGSQVGYQI 240
RFESTRSAAT KIVFLTVGLL LRQIQREPSL PQYEVLIVDE VHERHLHNDF LLGVLQRLLP 300
TRPDLKVILM SATINISLFS SYFSNAPVVQ VPGRLFPITV VYQPQEAEPT TSKSEKLDPR 360
PFLRVLESID HKYPPEERGD LLVFLSGMAE ISAVLEAAQT YASHTQRWVV LPLHSALSVA 420
DQDKVFDVAP PGVRKCILST NIAETSVTID GIRFVVDSGK VKEMSYDPQA KLQRLQEFWI 480
SQASAEQRKG RAGRTGPGVC FRLYAESDYD AFAPYPVPEI RRVALDSLVL QMKSMSVGDP 540
RTFPFIEPPP PASLETAILY LRDQGALDSS EALTPIGSLL AQLPVDVVIG KMLILGSMFS 600
LVEPVLTIAA ALSVQSPFTR SAQSSPECAA ARRPLESDQG DPFTLFNVFN AWVQVKSERS 660
RNSRKWCRRR GIEEHRLYEM ANLRRQFKEL LEDHGLLAGA QAAQVGDSYS RLQQRRERRA 720
LHQLKRQHEE GAGRRRKVLR LQEEQDGGSS DEDRAGPAPP GASDGVDIQD VKFKLRHDLA 780
QLQAAASSAQ DLSREQLALL KLVLGRGLYP QLAVPDAFNS SRKDSDQIFH TQAKQGAVLH 840
PTCVFAGSPE VLHAQELEAS NCDGSRDDKD KMSSKHQLLS FVSLLETNKP YLVNCVRIPA 900
LQSLLLFSRS LDTNGDCSRL VADGWLELQL ADSESAIRLL AASLRLRARW ESALDRQLAH 960
QAQQQLEEEE EDTPVSPKEV ATLSKELLQF TASKIPYSLR RLTGLEVQNM YVGPQTIPAT 1020
PHLPGLFGSS TLSPHPTKGG YAVTDFLTYN CLTNDTDLYS DCLRTFWTCP HCGLHAPLTP 1080
LERIAHENTC PQAPQDGPPG AEEAALETLQ KTSVLQRPYH CEACGKDFLF TPTEVLRHRK 1140
QHV 1143 
Gene Ontology
 GO:0005524; F:ATP binding; IEA:UniProtKB-KW.
 GO:0008026; F:ATP-dependent helicase activity; IEA:InterPro.
 GO:0003723; F:RNA binding; IEA:UniProtKB-KW. 
Interpro
 IPR011545; DNA/RNA_helicase_DEAD/DEAH_N.
 IPR011709; DUF1605.
 IPR007502; Helicase-assoc_dom.
 IPR014001; Helicase_ATP-bd.
 IPR001650; Helicase_C.
 IPR027417; P-loop_NTPase.
 IPR015880; Znf_C2H2-like. 
Pfam
 PF00270; DEAD
 PF04408; HA2
 PF00271; Helicase_C
 PF07717; OB_NTP_bind 
SMART
 SM00487; DEXDc
 SM00847; HA2
 SM00490; HELICc
 SM00355; ZnF_C2H2 
PROSITE
 PS00690; DEAH_ATP_HELICASE
 PS51192; HELICASE_ATP_BIND_1
 PS51194; HELICASE_CTER 
PRINTS