UniProt Accession

 MTNN_ECOLI; P0AF12; P24247 

Genbank Protein ID

 M31772; U24438; M83735; U70214; U00096; AP009048 

Genbank Nucleotide ID

 AAA23678.1; AAC38291.1; AAA24322.1; AAB08589.1; AAC73270.1; BAB96736.1 

Protein Name

 5'-methylthioadenosine/S-adenosylhomocysteine nucleosidase 

Protein Synonyms/Alias

 MTA/SAH nucleosidase; MTAN; 5'-methylthioadenosine nucleosidase; MTA nucleosidase; P46; S-adenosylhomocysteine nucleosidase; AdoHcy nucleosidase; SAH nucleosidase; SRH nucleosidase 

Gene Name

 mtnN 

Gene Synonyms/Alias

 mtn; pfs; yadA; b0159; JW0155 

Created Date

 July 27, 2013 

Organism

 Escherichia coli (strain K12) 

NCBI Taxa ID

 83333 

Lysine Modification

Position	Peptide	Type	References
120	PGCPAGFKADDKLIA	acetylation	[1]
124	AGFKADDKLIAAAEA	acetylation	[1, 2]
228	MVESLVQKLAHG***	acetylation	[1]

Reference

 [1] Acetyl-Phosphate Is a Critical Determinant of Lysine Acetylation in E. coli.
 Weinert BT, Iesmantavicius V, Wagner SA, Schölz C, Gummesson B, Beli P, Nyström T, Choudhary C.
 Mol Cell. 2013 Jul 25;51(2):265-72. [PMID: 23830618]
 [2] Comprehensive profiling of protein lysine acetylation in Escherichia coli.
 Zhang K, Zheng S, Yang JS, Chen Y, Cheng Z.
 J Proteome Res. 2013 Feb 1;12(2):844-51. [PMID: 23294111] 

Functional Description

 Catalyzes the irreversible cleavage of the glycosidic bond in both 5'-methylthioadenosine (MTA) and S- adenosylhomocysteine (SAH/AdoHcy) to adenine and the corresponding thioribose, 5'-methylthioribose and S-ribosylhomocysteine, respectively. Can also use 5'-isobutylthioadenosine, 5'-n- butylthioadenosine, S-adenosyl-D-homocysteine, decarboxylated adenosylhomocysteine, deaminated adenosylhomocysteine and S-2-aza- adenosylhomocysteine as substrates. 

Sequence Annotation

 REGION 173 174 Substrate binding.
 ACT_SITE 12 12 Proton acceptor (Probable).
 BINDING 78 78 Substrate; via amide nitrogen.
 BINDING 152 152 Substrate; via amide nitrogen and
 BINDING 197 197 Substrate.  

Keyword

 3D-structure; Amino-acid biosynthesis; Complete proteome; Hydrolase; Methionine biosynthesis; Reference proteome. 

Sequence Source

 UniProt (SWISSPROT/TrEMBL); GenBank; EMBL 

Protein Length

 232 AA 

Protein Sequence

Gene Ontology

 GO:0008782; F:adenosylhomocysteine nucleosidase activity; IDA:EcoCyc.
 GO:0008930; F:methylthioadenosine nucleosidase activity; IDA:EcoCyc.
 GO:0003001; P:generation of a signal involved in cell-cell signaling; IDA:EcoCyc.
 GO:0019284; P:L-methionine biosynthetic process from S-adenosylmethionine; IDA:EcoCyc.
 GO:0019509; P:L-methionine salvage from methylthioadenosine; IEA:UniProtKB-UniPathway.
 GO:0009164; P:nucleoside catabolic process; IEA:InterPro. 

Interpro

 IPR010049; MTA_SAH_Nsdase.
 IPR018017; Nucleoside_phosphorylase.
 IPR000845; Nucleoside_phosphorylase_d. 

Pfam

 PF01048; PNP_UDP_1 

SMART

  

PROSITE

  

PRINTS