CPLM 1.0 - Compendium of Protein Lysine Modification
TagContent
CPLM ID CPLM-013809
UniProt Accession
Genbank Protein ID
Genbank Nucleotide ID
Protein Name
 DDB1- and CUL4-associated factor 10 
Protein Synonyms/Alias
 WD repeat-containing protein 32 
Gene Name
 DCAF10 
Gene Synonyms/Alias
 WDR32 
Created Date
 July 27, 2013 
Organism
 Homo sapiens (Human) 
NCBI Taxa ID
 9606 
Lysine Modification
Position
Peptide
Type
References
128ARLFGWLKERSLGRGubiquitination[1, 2, 3, 4, 5]
204PISSKHIKTLSEAHEubiquitination[2, 4, 6]
421TSLQLHPKGWATLLRubiquitination[4]
476NVGRGYIKELCFSPDubiquitination[2, 4, 7]
502IRLLGFDKQCSELVDubiquitination[4, 7]
535NDVVLTTKFSPTHCQubiquitination[4]
558RVSLYQPKF******ubiquitination[2, 4]
Reference
 [1] A proteome-wide, quantitative survey of in vivo ubiquitylation sites reveals widespread regulatory roles.
 Wagner SA, Beli P, Weinert BT, Nielsen ML, Cox J, Mann M, Choudhary C.
 Mol Cell Proteomics. 2011 Oct;10(10):M111.013284. [PMID: 21890473]
 [2] Methods for quantification of in vivo changes in protein ubiquitination following proteasome and deubiquitinase inhibition.
 Udeshi ND, Mani DR, Eisenhaure T, Mertins P, Jaffe JD, Clauser KR, Hacohen N, Carr SA.
 Mol Cell Proteomics. 2012 May;11(5):148-59. [PMID: 22505724]
 [3] Systems-wide analysis of ubiquitylation dynamics reveals a key role for PAF15 ubiquitylation in DNA-damage bypass.
 Povlsen LK, Beli P, Wagner SA, Poulsen SL, Sylvestersen KB, Poulsen JW, Nielsen ML, Bekker-Jensen S, Mailand N, Choudhary C.
 Nat Cell Biol. 2012 Oct;14(10):1089-98. [PMID: 23000965]
 [4] Refined preparation and use of anti-diglycine remnant (K-ε-GG) antibody enables routine quantification of 10,000s of ubiquitination sites in single proteomics experiments.
 Udeshi ND, Svinkina T, Mertins P, Kuhn E, Mani DR, Qiao JW, Carr SA.
 Mol Cell Proteomics. 2013 Mar;12(3):825-31. [PMID: 23266961]
 [5] hCKSAAP_UbSite: improved prediction of human ubiquitination sites by exploiting amino acid pattern and properties.
 Chen Z, Zhou Y, Song J, Zhang Z.
 Biochim Biophys Acta. 2013 Aug;1834(8):1461-7. [PMID: 23603789]
 [6] Global identification of modular cullin-RING ligase substrates.
 Emanuele MJ, Elia AE, Xu Q, Thoma CR, Izhar L, Leng Y, Guo A, Chen YN, Rush J, Hsu PW, Yen HC, Elledge SJ.
 Cell. 2011 Oct 14;147(2):459-74. [PMID: 21963094]
 [7] Integrated proteomic analysis of post-translational modifications by serial enrichment.
 Mertins P, Qiao JW, Patel J, Udeshi ND, Clauser KR, Mani DR, Burgess MW, Gillette MA, Jaffe JD, Carr SA.
 Nat Methods. 2013 Jul;10(7):634-7. [PMID: 23749302
Functional Description
 May function as a substrate receptor for CUL4-DDB1 E3 ubiquitin-protein ligase complex. 
Sequence Annotation
 REPEAT 166 205 WD 1.
 REPEAT 209 247 WD 2.
 REPEAT 251 290 WD 3.
 REPEAT 296 335 WD 4.
 REPEAT 408 448 WD 5.
 REPEAT 470 508 WD 6.
 REPEAT 526 559 WD 7.  
Keyword
 Alternative splicing; Complete proteome; Reference proteome; Repeat; Ubl conjugation pathway; WD repeat. 
Sequence Source
 UniProt (SWISSPROT/TrEMBL); GenBank; EMBL 
Protein Length
 559 AA 
Protein Sequence
MFPFGPHSPG GDGSAGAGAE EPTPHEGQAA ATGPPSPLHP GADATHPPPP ARSPRRPGAP 60
SLSPAPRSGE LGLPGAPESS TASAPGEPSP PSPPCRRPGP DCRAKSRGRH GLGAGLGGPG 120
ARLFGWLKER SLGRGLFVDP ARDNFRTMTS LYGSIHPADS VYLSTRTHGA VFNLEYSPDG 180
SVLTVACEQT EVLLFDPISS KHIKTLSEAH EDCVNNIRFL DNRLFATCSD DTTIALWDLR 240
KLNTKVCTLH GHTSWVKNIE YDTNTRLLVT SGFDGNVIIW DTNRYTEDGC PHKKFFHTRF 300
LMRMRLTPDC SKMLISTSSG YLLILHDLDL TKSLEVGSYP ILRARRTTSS SDLTTSSSSS 360
GPRVSGSPCH HSDSNSSEKH MSRASQREGV SPRNSLEVVT PEVLGESDHG NCITSLQLHP 420
KGWATLLRCS SNSDDEECTC VYEFQEGAPV RPVSPRCSLR LTHYIEEANV GRGYIKELCF 480
SPDGRMISSP HGYGIRLLGF DKQCSELVDC LPKEASPLRV IRSLYSHNDV VLTTKFSPTH 540
CQIASGCLSG RVSLYQPKF 559 
Gene Ontology
 GO:0080008; C:Cul4-RING ubiquitin ligase complex; IDA:UniProtKB.
 GO:0016567; P:protein ubiquitination; IC:UniProtKB. 
Interpro
 IPR015943; WD40/YVTN_repeat-like_dom.
 IPR001680; WD40_repeat.
 IPR019775; WD40_repeat_CS.
 IPR017986; WD40_repeat_dom. 
Pfam
 PF00400; WD40 
SMART
 SM00320; WD40 
PROSITE
 PS00678; WD_REPEATS_1
 PS50082; WD_REPEATS_2
 PS50294; WD_REPEATS_REGION 
PRINTS