CPLM 1.0 - Compendium of Protein Lysine Modification
TagContent
CPLM ID CPLM-010268
UniProt Accession
Genbank Protein ID
Genbank Nucleotide ID
Protein Name
 NAD-dependent dihydropyrimidine dehydrogenase subunit PreT 
Protein Synonyms/Alias
 DPD; Dihydrothymine dehydrogenase; Dihydrouracil dehydrogenase 
Gene Name
 preT 
Gene Synonyms/Alias
 yeiT; b2146; JW2133 
Created Date
 July 27, 2013 
Organism
 Escherichia coli (strain K12) 
NCBI Taxa ID
 83333 
Lysine Modification
Position
Peptide
Type
References
364EPQRNTIKTQNYQTRpupylation[1]
Reference
 [1] Reconstitution of the Mycobacterium tuberculosis pupylation pathway in Escherichia coli.
 Cerda-Maira FA, McAllister F, Bode NJ, Burns KE, Gygi SP, Darwin KH.
 EMBO Rep. 2011 Jul 8;12(8):863-70. [PMID: 21738222] 
Functional Description
 Involved in pyrimidine base degradation. Catalyzes physiologically the reduction of uracil to 5,6-dihydrouracil (DHU) by using NADH as a specific cosubstrate. It also catalyzes the reverse reaction and the reduction of thymine to 5,6- dihydrothymine (DHT). 
Sequence Annotation
 BINDING 286 286 NAD (Probable).  
Keyword
 Complete proteome; Direct protein sequencing; NAD; Oxidoreductase; Reference proteome. 
Sequence Source
 UniProt (SWISSPROT/TrEMBL); GenBank; EMBL 
Protein Length
 412 AA 
Protein Sequence
MPQQNYLDEL TPAFTSLLAI KEASRCLLCH DAPCSQACPA QTDPGKFIRS IYFRNFKGAA 60
ETIRENNALG AVCARVCPTE KLCQSGCTRA GVDAPIDIGR LQRFVTDFEQ QTGMEIYQPG 120
TKTLGKVAII GAGPAGLQAS VTLTNQGYDV TIYEKEAHPG GWLRNGIPQF RLPQSVLDAE 180
IARIEKMGVT IKCNNEVGNT LTLEQLKAEN RAVLVTVGLS SGSGLPLFEH SDVEIAVDFL 240
QRARQAQGDI SIPQSALIIG GGDVAMDVAS TLKVLGCQAV TCVAREELDE FPASEKEFTS 300
ARELGVSIID GFTPVAVEGN KVTFKHVRLS GELTMAADKI ILAVGQHARL DAFAELEPQR 360
NTIKTQNYQT RDPQVFAAGD IVEGDKTVVY AVKTGKEAAE AIHHYLEGAC SC 412 
Gene Ontology
 GO:0004159; F:dihydrouracil dehydrogenase (NAD+) activity; IEA:EC.
 GO:0050660; F:flavin adenine dinucleotide binding; IEA:InterPro.
 GO:0051536; F:iron-sulfur cluster binding; IDA:EcoCyc.
 GO:0003954; F:NADH dehydrogenase activity; IDA:UniProtKB.
 GO:0006208; P:pyrimidine nucleobase catabolic process; IDA:UniProtKB. 
Interpro
 IPR012285; Fum_reductase_C.
 IPR009051; Helical_ferredxn.
 IPR016040; NAD(P)-bd_dom.
 IPR023753; Pyr_nucl-diS_OxRdtase_FAD/NAD.
 IPR001327; Pyr_OxRdtase_NAD-bd_dom. 
Pfam
 PF00070; Pyr_redox
 PF07992; Pyr_redox_2 
SMART
  
PROSITE
  
PRINTS