UniProt Accession

 GDIR2_HUMAN; P52566; B5BU79 

Genbank Protein ID

 L20688; X69549; L07916; AF498927; AB451315; AB451445; CH471094; BC009200 

Genbank Nucleotide ID

 AAA59539.1; CAA49280.1; AAM21075.1; BAG70129.1; BAG70259.1; EAW96336.1; AAH09200.1 

Protein Name

 Rho GDP-dissociation inhibitor 2 

Protein Synonyms/Alias

 Rho GDI 2; Ly-GDI; Rho-GDI beta 

Gene Name

 ARHGDIB 

Gene Synonyms/Alias

 GDIA2; GDID4; RAP1GN1 

Created Date

 July 27, 2013 

Organism

 Homo sapiens (Human) 

NCBI Taxa ID

 9606 

Lysine Modification

Position	Peptide	Type	References
21	DDDELDSKLNYKPPP	acetylation	[1]
21	DDDELDSKLNYKPPP	ubiquitination	[2, 3]
25	LDSKLNYKPPPQKSL	acetylation	[1]
25	LDSKLNYKPPPQKSL	ubiquitination	[2, 3]
30	NYKPPPQKSLKELQE	acetylation	[1]
30	NYKPPPQKSLKELQE	ubiquitination	[3]
33	PPPQKSLKELQEMDK	ubiquitination	[2, 3]
40	KELQEMDKDDESLIK	acetylation	[1]
40	KELQEMDKDDESLIK	ubiquitination	[3]
47	KDDESLIKYKKTLLG	acetylation	[1]
47	KDDESLIKYKKTLLG	ubiquitination	[3]
49	DESLIKYKKTLLGDG	ubiquitination	[3]
50	ESLIKYKKTLLGDGP	ubiquitination	[2, 3, 4, 5]
63	GPVVTDPKAPNVVVT	ubiquitination	[2, 3]
95	TGDLEALKKETIVLK	ubiquitination	[2]
96	GDLEALKKETIVLKE	ubiquitination	[3]
102	KKETIVLKEGSEYRV	acetylation	[1]
102	KKETIVLKEGSEYRV	ubiquitination	[3]
110	EGSEYRVKIHFKVNR	ubiquitination	[3]
114	YRVKIHFKVNRDIVS	ubiquitination	[3]
124	RDIVSGLKYVQHTYR	acetylation	[1]
124	RDIVSGLKYVQHTYR	ubiquitination	[2, 3, 4, 5]
135	HTYRTGVKVDKATFM	ubiquitination	[2]
138	RTGVKVDKATFMVGS	ubiquitination	[2, 3]
164	TPVEEAPKGMLARGT	ubiquitination	[3]
175	ARGTYHNKSFFTDDD	acetylation	[1]
175	ARGTYHNKSFFTDDD	ubiquitination	[3]

Reference

 [1] Lysine acetylation targets protein complexes and co-regulates major cellular functions.
 Choudhary C, Kumar C, Gnad F, Nielsen ML, Rehman M, Walther TC, Olsen JV, Mann M.
 Science. 2009 Aug 14;325(5942):834-40. [PMID: 19608861]
 [2] Methods for quantification of in vivo changes in protein ubiquitination following proteasome and deubiquitinase inhibition.
 Udeshi ND, Mani DR, Eisenhaure T, Mertins P, Jaffe JD, Clauser KR, Hacohen N, Carr SA.
 Mol Cell Proteomics. 2012 May;11(5):148-59. [PMID: 22505724]
 [3] Refined preparation and use of anti-diglycine remnant (K-ε-GG) antibody enables routine quantification of 10,000s of ubiquitination sites in single proteomics experiments.
 Udeshi ND, Svinkina T, Mertins P, Kuhn E, Mani DR, Qiao JW, Carr SA.
 Mol Cell Proteomics. 2013 Mar;12(3):825-31. [PMID: 23266961]
 [4] A proteome-wide, quantitative survey of in vivo ubiquitylation sites reveals widespread regulatory roles.
 Wagner SA, Beli P, Weinert BT, Nielsen ML, Cox J, Mann M, Choudhary C.
 Mol Cell Proteomics. 2011 Oct;10(10):M111.013284. [PMID: 21890473]
 [5] hCKSAAP_UbSite: improved prediction of human ubiquitination sites by exploiting amino acid pattern and properties.
 Chen Z, Zhou Y, Song J, Zhang Z.
 Biochim Biophys Acta. 2013 Aug;1834(8):1461-7. [PMID: 23603789] 

Functional Description

 Regulates the GDP/GTP exchange reaction of the Rho proteins by inhibiting the dissociation of GDP from them, and the subsequent binding of GTP to them. 

Sequence Annotation

 MOD_RES 2 2 N-acetylthreonine.
 MOD_RES 21 21 N6-acetyllysine.
 MOD_RES 24 24 Phosphotyrosine.
 MOD_RES 25 25 N6-acetyllysine.
 MOD_RES 40 40 N6-acetyllysine.
 MOD_RES 47 47 N6-acetyllysine.
 MOD_RES 102 102 N6-acetyllysine.
 MOD_RES 124 124 N6-acetyllysine.
 MOD_RES 145 145 Phosphoserine.
 MOD_RES 175 175 N6-acetyllysine.  

Keyword

 3D-structure; Acetylation; Complete proteome; Cytoplasm; Direct protein sequencing; GTPase activation; Phosphoprotein; Reference proteome. 

Sequence Source

 UniProt (SWISSPROT/TrEMBL); GenBank; EMBL 

Protein Length

 201 AA 

Protein Sequence

Gene Ontology

 GO:0016023; C:cytoplasmic membrane-bounded vesicle; TAS:ProtInc.
 GO:0005856; C:cytoskeleton; TAS:UniProtKB.
 GO:0005829; C:cytosol; TAS:Reactome.
 GO:0005096; F:GTPase activator activity; IEA:UniProtKB-KW.
 GO:0005094; F:Rho GDP-dissociation inhibitor activity; TAS:ProtInc.
 GO:0030036; P:actin cytoskeleton organization; TAS:ProtInc.
 GO:0006928; P:cellular component movement; TAS:UniProtKB.
 GO:0006955; P:immune response; TAS:ProtInc.
 GO:0007275; P:multicellular organismal development; TAS:ProtInc.
 GO:0007162; P:negative regulation of cell adhesion; TAS:ProtInc.
 GO:0043547; P:positive regulation of GTPase activity; IEA:GOC.
 GO:0051056; P:regulation of small GTPase mediated signal transduction; TAS:Reactome.
 GO:0007266; P:Rho protein signal transduction; TAS:ProtInc. 

Interpro

 IPR014756; Ig_E-set.
 IPR000406; Rho_GDI.
 IPR024792; RhoGDI_domain. 

Pfam

 PF02115; Rho_GDI 

SMART

  

PROSITE

  

PRINTS

 PR00492; RHOGDI.