CPLM 1.0 - Compendium of Protein Lysine Modification
TagContent
CPLM ID CPLM-022569
UniProt Accession
Genbank Protein ID
Genbank Nucleotide ID
Protein Name
 WD repeat-containing protein 35 
Protein Synonyms/Alias
 Intraflagellar transport protein 121 homolog 
Gene Name
 WDR35 
Gene Synonyms/Alias
 IFT121; KIAA1336 
Created Date
 July 27, 2013 
Organism
 Homo sapiens (Human) 
NCBI Taxa ID
 9606 
Lysine Modification
Position
Peptide
Type
References
24LQCVSWNKEQGFIACubiquitination[1]
42DGLLKVLKLETQTDDubiquitination[1]
51ETQTDDAKLRGLAAPubiquitination[1, 2]
300GEHLGTLKVPGKEISubiquitination[2]
459WQYRVAKKLTALEINubiquitination[1]
498DGVLDYSKTIQGTRDubiquitination[1, 2]
590QVVGELLKLERRDVWubiquitination[1, 2]
680DSRALIEKVGIKDASubiquitination[1, 2]
684LIEKVGIKDASQFIEubiquitination[1, 2]
709LAEAALQKLDLYTAEubiquitination[1, 2]
736KFVKRLGKLLSESMKubiquitination[1]
743KLLSESMKQAEVVGYubiquitination[2]
776LAIGLRLKLGDWFRVubiquitination[1, 2, 3, 4]
788FRVLQLLKTGSGDADubiquitination[2]
815DYFADRQKWLNAVQYubiquitination[3, 4]
900VHLNQWNKAVELAKNubiquitination[1]
948NYFFDAAKLMFKIADubiquitination[5]
996QRGKVKGKSSEATSAubiquitination[2]
1086RAFGTCSKAFIKLKSubiquitination[1]
1102ETLSSEQKQQYEDLAubiquitination[2, 5]
1115LALEIFTKHTSKDNRubiquitination[5]
1119IFTKHTSKDNRKPELubiquitination[1]
Reference
 [1] Refined preparation and use of anti-diglycine remnant (K-ε-GG) antibody enables routine quantification of 10,000s of ubiquitination sites in single proteomics experiments.
 Udeshi ND, Svinkina T, Mertins P, Kuhn E, Mani DR, Qiao JW, Carr SA.
 Mol Cell Proteomics. 2013 Mar;12(3):825-31. [PMID: 23266961]
 [2] Systematic and quantitative assessment of the ubiquitin-modified proteome.
 Kim W, Bennett EJ, Huttlin EL, Guo A, Li J, Possemato A, Sowa ME, Rad R, Rush J, Comb MJ, Harper JW, Gygi SP.
 Mol Cell. 2011 Oct 21;44(2):325-40. [PMID: 21906983]
 [3] A proteome-wide, quantitative survey of in vivo ubiquitylation sites reveals widespread regulatory roles.
 Wagner SA, Beli P, Weinert BT, Nielsen ML, Cox J, Mann M, Choudhary C.
 Mol Cell Proteomics. 2011 Oct;10(10):M111.013284. [PMID: 21890473]
 [4] hCKSAAP_UbSite: improved prediction of human ubiquitination sites by exploiting amino acid pattern and properties.
 Chen Z, Zhou Y, Song J, Zhang Z.
 Biochim Biophys Acta. 2013 Aug;1834(8):1461-7. [PMID: 23603789]
 [5] Global identification of modular cullin-RING ligase substrates.
 Emanuele MJ, Elia AE, Xu Q, Thoma CR, Izhar L, Leng Y, Guo A, Chen YN, Rush J, Hsu PW, Yen HC, Elledge SJ.
 Cell. 2011 Oct 14;147(2):459-74. [PMID: 21963094] 
Functional Description
 Component of the IFT complex A (IFT-A), a complex required for retrograde ciliary transport. Required for ciliogenesis. May promote CASP3 activation and TNF-stimulated apoptosis. 
Sequence Annotation
 REPEAT 12 51 WD 1.
 REPEAT 69 108 WD 2.
 REPEAT 113 152 WD 3.
 REPEAT 154 193 WD 4.
 REPEAT 502 539 WD 5.  
Keyword
 Alternative splicing; Cell projection; Ciliopathy; Cilium; Cilium biogenesis/degradation; Complete proteome; Cytoplasm; Cytoskeleton; Disease mutation; Ectodermal dysplasia; Polymorphism; Reference proteome; Repeat; WD repeat. 
Sequence Source
 UniProt (SWISSPROT/TrEMBL); GenBank; EMBL 
Protein Length
 1181 AA 
Protein Sequence
MFFYLSKKIS IPNNVKLQCV SWNKEQGFIA CGGEDGLLKV LKLETQTDDA KLRGLAAPSN 60
LSMNQTLEGH SGSVQVVTWN EQYQKLTTSD ENGLIIVWML YKGSWIEEMI NNRNKSVVRS 120
MSWNADGQKI CIVYEDGAVI VGSVDGNRIW GKDLKGIQLS HVTWSADSKV LLFGMANGEI 180
HIYDNQGNFM IKMKLSCLVN VTGAISIAGI HWYHGTEGYV EPDCPCLAVC FDNGRCQIMR 240
HENDQNPVLI DTGMYVVGIQ WNHMGSVLAV AGFQKAAMQD KDVNIVQFYT PFGEHLGTLK 300
VPGKEISALS WEGGGLKIAL AVDSFIYFAN IRPNYKWGYC SNTVVYAYTR PDRPEYCVVF 360
WDTKNNEKYV KYVKGLISIT TCGDFCILAT KADENHPQEE NEMETFGATF VLVLCNSIGT 420
PLDPKYIDIV PLFVAMTKTH VIAASKEAFY TWQYRVAKKL TALEINQITR SRKEGRERIY 480
HVDDTPSGSM DGVLDYSKTI QGTRDPICAI TASDKILIVG RESGTIQRYS LPNVGLIQKY 540
SLNCRAYQLS LNCNSSRLAI IDISGVLTFF DLDARVTDST GQQVVGELLK LERRDVWDMK 600
WAKDNPDLFA MMEKTRMYVF RNLDPEEPIQ TSGYICNFED LEIKSVLLDE ILKDPEHPNK 660
DYLINFEIRS LRDSRALIEK VGIKDASQFI EDNPHPRLWR LLAEAALQKL DLYTAEQAFV 720
RCKDYQGIKF VKRLGKLLSE SMKQAEVVGY FGRFEEAERT YLEMDRRDLA IGLRLKLGDW 780
FRVLQLLKTG SGDADDSLLE QANNAIGDYF ADRQKWLNAV QYYVQGRNQE RLAECYYMLE 840
DYEGLENLAI SLPENHKLLP EIAQMFVRVG MCEQAVTAFL KCSQPKAAVD TCVHLNQWNK 900
AVELAKNHSM KEIGSLLARY ASHLLEKNKT LDAIELYRKA NYFFDAAKLM FKIADEEAKK 960
GSKPLRVKKL YVLSALLIEQ YHEQMKNAQR GKVKGKSSEA TSALAGLLEE EVLSTTDRFT 1020
DNAWRGAEAY HFFILAQRQL YEGCVDTALK TALHLKDYED IIPPVEIYSL LALCACASRA 1080
FGTCSKAFIK LKSLETLSSE QKQQYEDLAL EIFTKHTSKD NRKPELDSLM EGGEGKLPTC 1140
VATGSPITEY QFWMCSVCKH GVLAQEISHY SFCPLCHSPV G 1181 
Gene Ontology
 GO:0005813; C:centrosome; ISS:UniProtKB.
 GO:0035085; C:cilium axoneme; ISS:UniProtKB.
 GO:0030991; C:intraflagellar transport particle A; IDA:UniProtKB.
 GO:0005932; C:microtubule basal body; ISS:UniProtKB.
 GO:0042384; P:cilium assembly; IMP:UniProtKB. 
Interpro
 IPR015943; WD40/YVTN_repeat-like_dom.
 IPR001680; WD40_repeat.
 IPR017986; WD40_repeat_dom.
 IPR017233; WD_repeat_p35. 
Pfam
 PF00400; WD40 
SMART
 SM00320; WD40 
PROSITE
 PS00678; WD_REPEATS_1
 PS50082; WD_REPEATS_2
 PS50294; WD_REPEATS_REGION 
PRINTS