CPLM 1.0 - Compendium of Protein Lysine Modification
TagContent
CPLM ID CPLM-030866
UniProt Accession
Genbank Protein ID
Genbank Nucleotide ID
Protein Name
 Tuberin 
Protein Synonyms/Alias
 cDNA FLJ59538, highly similar to Tuberin 
Gene Name
 TSC2 
Gene Synonyms/Alias
  
Created Date
 July 27, 2013 
Organism
 Homo sapiens (Human) 
NCBI Taxa ID
 9606 
Lysine Modification
Position
Peptide
Type
References
20GQICEVAKTKKFEEHubiquitination[1, 2]
22ICEVAKTKKFEEHAVubiquitination[2]
23CEVAKTKKFEEHAVEubiquitination[2, 3]
57HAVLALLKAIVQGQGubiquitination[2]
79ALFFKVIKDYPSNEDubiquitination[2, 4]
95HERLEVFKALTDNGRubiquitination[1, 2, 5]
201LCRTINVKELCEPCWubiquitination[2, 4]
209ELCEPCWKLMRNLLGubiquitination[2, 4]
389AQSIHPAKDGWIQNLubiquitination[1, 2, 3, 4, 5, 6]
585HRLGLPNKDGVVRFSubiquitination[2, 6]
609PERGSEKKTSGPLSPubiquitination[2, 4, 6, 7]
733SYHNYLDKTKQREMVubiquitination[2]
865DFVPFITKGLRSNVLubiquitination[1, 2, 3, 4, 5, 6, 7]
881SFDDTPEKDSFRARSubiquitination[2, 4, 5, 6]
924AQADDSLKNLHLELTubiquitination[1]
951SNFTAVPKRSPVGEFubiquitination[2, 4]
966LLAGGRTKTWLVGNKubiquitination[2]
1014GVHVRQTKEAPAKLEubiquitination[2]
1019QTKEAPAKLESQAGQubiquitination[2, 4, 6]
1076APAAKPEKASAGTRVubiquitination[2]
1088TRVPVQEKTNLAAYVubiquitination[2]
1150LMAAERFKEHRDTALubiquitination[2]
1159HRDTALYKSLSVPAAubiquitination[2, 4]
1170VPAASTAKPPPLPRSubiquitination[2]
1470LGRLIELKDCQPDKVubiquitination[2]
1543NDSGEDFKLGTIKGQubiquitination[2]
1574LVSLQCRKDMEGLVDubiquitination[2]
1586LVDTSVAKIVSDRNLubiquitination[2, 7]
1624PTDIYPSKWIARLRHubiquitination[2, 4, 7]
1660VHPPSHSKAPAQTPAubiquitination[1, 2]
Reference
 [1] Global identification of modular cullin-RING ligase substrates.
 Emanuele MJ, Elia AE, Xu Q, Thoma CR, Izhar L, Leng Y, Guo A, Chen YN, Rush J, Hsu PW, Yen HC, Elledge SJ.
 Cell. 2011 Oct 14;147(2):459-74. [PMID: 21963094]
 [2] Refined preparation and use of anti-diglycine remnant (K-ε-GG) antibody enables routine quantification of 10,000s of ubiquitination sites in single proteomics experiments.
 Udeshi ND, Svinkina T, Mertins P, Kuhn E, Mani DR, Qiao JW, Carr SA.
 Mol Cell Proteomics. 2013 Mar;12(3):825-31. [PMID: 23266961]
 [3] A proteome-wide, quantitative survey of in vivo ubiquitylation sites reveals widespread regulatory roles.
 Wagner SA, Beli P, Weinert BT, Nielsen ML, Cox J, Mann M, Choudhary C.
 Mol Cell Proteomics. 2011 Oct;10(10):M111.013284. [PMID: 21890473]
 [4] Integrated proteomic analysis of post-translational modifications by serial enrichment.
 Mertins P, Qiao JW, Patel J, Udeshi ND, Clauser KR, Mani DR, Burgess MW, Gillette MA, Jaffe JD, Carr SA.
 Nat Methods. 2013 Jul;10(7):634-7. [PMID: 23749302]
 [5] Systematic and quantitative assessment of the ubiquitin-modified proteome.
 Kim W, Bennett EJ, Huttlin EL, Guo A, Li J, Possemato A, Sowa ME, Rad R, Rush J, Comb MJ, Harper JW, Gygi SP.
 Mol Cell. 2011 Oct 21;44(2):325-40. [PMID: 21906983]
 [6] Landscape of the PARKIN-dependent ubiquitylome in response to mitochondrial depolarization.
 Sarraf SA, Raman M, Guarani-Pereira V, Sowa ME, Huttlin EL, Gygi SP, Harper JW.
 Nature. 2013 Apr 18;496(7445):372-6. [PMID: 23503661]
 [7] Methods for quantification of in vivo changes in protein ubiquitination following proteasome and deubiquitinase inhibition.
 Udeshi ND, Mani DR, Eisenhaure T, Mertins P, Jaffe JD, Clauser KR, Hacohen N, Carr SA.
 Mol Cell Proteomics. 2012 May;11(5):148-59. [PMID: 22505724
Functional Description
  
Sequence Annotation
  
Keyword
 Complete proteome; Reference proteome. 
Sequence Source
 UniProt (SWISSPROT/TrEMBL); GenBank; EMBL 
Protein Length
 1692 AA 
Protein Sequence
MECGLNNRIR MIGQICEVAK TKKFEEHAVE ALWKAVADLL QPERPLEARH AVLALLKAIV 60
QGQGERLGVL RALFFKVIKD YPSNEDLHER LEVFKALTDN GRHITYLEEE LADFVLQWMD 120
VGLSSEFLLV LVNLVKFNSC YLDEYIARMV QMICLLCVRT ASSVDIEVSL QVLDAVVCYN 180
CLPAESLPLF IVTLCRTINV KELCEPCWKL MRNLLGTHLG HSAIYNMCHL MEDRAYMEDA 240
PLLRGAVFFV GMALWGAHRL YSLRNSPTSV LPSFYQAMAC PNEVVSYEIV LSITRLIKKY 300
RKELQVVAWD ILLNIIERLL QQLQTLDSPE LRTIVHDLLT TVEELCDQNE FHGSQERYFE 360
LVERCADQRP ESSLLNLISY RAQSIHPAKD GWIQNLQALM ERFFRSESRG AVRIKVLDVL 420
SFVLLINRQF YEEELINSVV ISQLSHIPED KDHQVRKLAT QLLVDLAEGC HTHHFNSLLD 480
IIEKVMARSL SPPPELEERD VAAYSASLED VKTAVLGLLV ILQTKLYTLP ASHATRVYEM 540
LVSHIQLHYK HSYTLPIASS IRLQAFDFLL LLRADSLHRL GLPNKDGVVR FSPYCVCDYM 600
EPERGSEKKT SGPLSPPTGP PGPAPAGPAV RLGSVPYSLL FRVLLQCLKQ ESDWKVLKLV 660
LGRLPESLRY KVLIFTSPCS VDQLCSALCS MLSGPKTLER LRGAPEGFSR TDLHLAVVPV 720
LTALISYHNY LDKTKQREMV YCLEQGLIHR CASQCVVALS ICSVEMPDII IKALPVLVVK 780
LTHISATASM AVPLLEFLST LARLPHLYRN FAAEQYASVF AISLPYTNPS KFNQYIVCLA 840
HHVIAMWFIR CRLPFRKDFV PFITKGLRSN VLLSFDDTPE KDSFRARSTS LNERPKSRIQ 900
TSLTSASLGS ADENSVAQAD DSLKNLHLEL TETCLDMMAR YVFSNFTAVP KRSPVGEFLL 960
AGGRTKTWLV GNKLVTVTTS VGTGTRSLLG LDSGELQSGP ESSSSPGVHV RQTKEAPAKL 1020
ESQAGQQVSR GARDRVRSMS GGHGLRVGAL DVPASQFLGS ATSPGPRTAP AAKPEKASAG 1080
TRVPVQEKTN LAAYVPLLTQ GWAEILVRRP TGNTSWLMSL ENPLSPFSSD INNMPLQELS 1140
NALMAAERFK EHRDTALYKS LSVPAASTAK PPPLPRSNTD SAVVMEEGSP GEVPVLVEPP 1200
GLEDVEAALG MDRRTDAYSR SSSVSSQEEK SLHAEELVGR GIPIERVVSS EGGRPSVDLS 1260
FQPSQPLSKS SSSPELQTLQ DILGDPGDKA DVGRLSPEVK ARSQSGTLDG ESAAWSASGE 1320
DSRGQPEGPL PSSSPRSPSG LRPRGYTISD SAPSRRGKRV ERDALKSRAT ASNAEKVPGI 1380
NPSFVFLQLY HSPFFGDESN KPILLPNESQ SFERSVQLLD QIPSYDTHKI AVLYVGEGQS 1440
NSELAILSNE HGSYRYTEFL TGLGRLIELK DCQPDKVYLG GLDVCGEDGQ FTYCWHDDIM 1500
QAVFHIATLM PTKDVDKHRC DKKRHLGNDF VSIVYNDSGE DFKLGTIKGQ FNFVHVIVTP 1560
LDYECNLVSL QCRKDMEGLV DTSVAKIVSD RNLPFVARQM ALHANMASQV HHSRSNPTDI 1620
YPSKWIARLR HIKRLRQRIC EEAAYSNPSL PLVHPPSHSK APAQTPAEPT PGYEVGQRKR 1680
LISSVEDFTE FV 1692 
Gene Ontology
 GO:0005737; C:cytoplasm; IDA:HPA.
 GO:0005634; C:nucleus; IEA:InterPro.
 GO:0033596; C:TSC1-TSC2 complex; IEA:InterPro.
 GO:0005096; F:GTPase activator activity; IEA:InterPro.
 GO:0032007; P:negative regulation of TOR signaling cascade; IEA:InterPro.
 GO:0051056; P:regulation of small GTPase mediated signal transduction; IEA:InterPro. 
Interpro
 IPR011989; ARM-like.
 IPR016024; ARM-type_fold.
 IPR000331; Rap_GAP_dom.
 IPR003913; Tuberin.
 IPR018515; Tuberin-type_domain.
 IPR027107; Tuberin/Ral-act_asu.
 IPR024584; Tuberin_N. 
Pfam
 PF11864; DUF3384
 PF02145; Rap_GAP
 PF03542; Tuberin 
SMART
  
PROSITE
 PS50085; RAPGAP 
PRINTS
 PR01431; TUBERIN.