CPLM 1.0 - Compendium of Protein Lysine Modification
TagContent
CPLM ID CPLM-008545
UniProt Accession
Genbank Protein ID
Genbank Nucleotide ID
Protein Name
 Diacylglycerol kinase theta 
Protein Synonyms/Alias
 DAG kinase theta; Diglyceride kinase theta; DGK-theta 
Gene Name
 DGKQ 
Gene Synonyms/Alias
 DAGK4 
Created Date
 July 27, 2013 
Organism
 Homo sapiens (Human) 
NCBI Taxa ID
 9606 
Lysine Modification
Position
Peptide
Type
References
202ARCEVCRKTCGSSDVubiquitination[1]
258LLPGGFSKTQSFRIVubiquitination[1]
297QATPESGKQTLKIFDubiquitination[1]
400PRAQEVLKIYPGWLKubiquitination[1]
Reference
 [1] Refined preparation and use of anti-diglycine remnant (K-ε-GG) antibody enables routine quantification of 10,000s of ubiquitination sites in single proteomics experiments.
 Udeshi ND, Svinkina T, Mertins P, Kuhn E, Mani DR, Qiao JW, Carr SA.
 Mol Cell Proteomics. 2013 Mar;12(3):825-31. [PMID: 23266961] 
Functional Description
 Phosphorylates diacylglycerol (DAG) to generate phosphatidic acid (PA). May regulate the activity of protein kinase C by controlling the balance between these two signaling lipids. Activated in the nucleus in response to alpha-thrombin and nerve growth factor (By similarity). May be involved in cAMP- induced activation of NR5A1 and subsequent steroidogenic gene transcription by delivering PA as ligand for NR5A1. Acts synergistically with NR5A1 on CYP17 transcriptional activity. 
Sequence Annotation
 DOMAIN 395 494 Ras-associating.
 DOMAIN 584 721 DAGKc.
 ZN_FING 60 108 Phorbol-ester/DAG-type 1.
 ZN_FING 121 168 Phorbol-ester/DAG-type 2.
 ZN_FING 183 234 Phorbol-ester/DAG-type 3.
 MOTIF 555 559 LXXLL motif 1.
 MOTIF 574 578 LXXLL motif 2.
 MOD_RES 22 22 Phosphoserine (By similarity).
 MOD_RES 26 26 Phosphoserine (By similarity).  
Keyword
 ATP-binding; Cell membrane; Complete proteome; Cytoplasm; Cytoskeleton; Kinase; Membrane; Metal-binding; Nucleotide-binding; Nucleus; Phosphoprotein; Polymorphism; Reference proteome; Repeat; Transferase; Zinc; Zinc-finger. 
Sequence Source
 UniProt (SWISSPROT/TrEMBL); GenBank; EMBL 
Protein Length
 942 AA 
Protein Sequence
MAAAAEPGAR AWLGGGSPRP GSPACSPVLG SGGRARPGPG PGPGPERAGV RAPGPAAAPG 60
HSFRKVTLTK PTFCHLCSDF IWGLAGFLCD VCNFMSHEKC LKHVRIPCTS VAPSLVRVPV 120
AHCFGPRGLH KRKFCAVCRK VLEAPALHCE VCELHLHPDC VPFACSDCRQ CHQDGHQDHD 180
THHHHWREGN LPSGARCEVC RKTCGSSDVL AGVRCEWCGV QAHSLCSAAL APECGFGRLR 240
SLVLPPACVR LLPGGFSKTQ SFRIVEAAEP GEGGDGADGS AAVGPGRETQ ATPESGKQTL 300
KIFDGDDAVR RSQFRLVTVS RLAGAEEVLE AALRAHHIPE DPGHLELCRL PPSSQACDAW 360
AGGKAGSAVI SEEGRSPGSG EATPEAWVIR ALPRAQEVLK IYPGWLKVGV AYVSVRVTPK 420
STARSVVLEV LPLLGRQAES PESFQLVEVA MGCRHVQRTM LMDEQPLLDR LQDIRQMSVR 480
QVSQTRFYVA ESRDVAPHVS LFVGGLPPGL SPEEYSSLLH EAGATKATVV SVSHIYSSQG 540
AVVLDVACFA EAERLYMLLK DMAVRGRLLT ALVLPDLLHA KLPPDSCPLL VFVNPKSGGL 600
KGRDLLCSFR KLLNPHQVFD LTNGGPLPGL HLFSQVPCFR VLVCGGDGTV GWVLGALEET 660
RYRLACPEPS VAILPLGTGN DLGRVLRWGA GYSGEDPFSV LLSVDEADAV LMDRWTILLD 720
AHEAGSAEND TADAEPPKIV QMSNYCGIGI DAELSLDFHQ AREEEPGKFT SRLHNKGVYV 780
RVGLQKISHS RSLHKQIRLQ VERQEVELPS IEGLIFINIP SWGSGADLWG SDSDTRFEKP 840
RMDDGLLEVV GVTGVVHMGQ VQGGLRSGIR IAQGSYFRVT LLKATPVQVD GEPWVQAPGH 900
MIISAAGPKV HMLRKAKQKP RRAGTTRDAR ADAAPAPESD PR 942 
Gene Ontology
 GO:0005856; C:cytoskeleton; IDA:UniProtKB.
 GO:0005829; C:cytosol; IDA:UniProtKB.
 GO:0016607; C:nuclear speck; IDA:UniProtKB.
 GO:0005886; C:plasma membrane; IDA:UniProtKB.
 GO:0005524; F:ATP binding; IEA:UniProtKB-KW.
 GO:0004143; F:diacylglycerol kinase activity; IDA:UniProtKB.
 GO:0046872; F:metal ion binding; IEA:UniProtKB-KW.
 GO:0019933; P:cAMP-mediated signaling; IDA:UniProtKB.
 GO:0030168; P:platelet activation; TAS:Reactome.
 GO:0070528; P:protein kinase C signaling cascade; IDA:UniProtKB.
 GO:0007205; P:protein kinase C-activating G-protein coupled receptor signaling pathway; IEA:InterPro.
 GO:0033198; P:response to ATP; IDA:UniProtKB.
 GO:0070493; P:thrombin receptor signaling pathway; IDA:UniProtKB. 
Interpro
 IPR000756; Diacylglycerol_kin_accessory.
 IPR001206; Diacylglycerol_kinase_cat_dom.
 IPR002219; Prot_Kinase_C-like_PE/DAG-bd.
 IPR000159; Ras-assoc. 
Pfam
 PF00130; C1_1
 PF00609; DAGK_acc
 PF00781; DAGK_cat
 PF00788; RA 
SMART
 SM00109; C1
 SM00045; DAGKa
 SM00046; DAGKc
 SM00314; RA 
PROSITE
 PS50146; DAGK
 PS50200; RA
 PS00479; ZF_DAG_PE_1
 PS50081; ZF_DAG_PE_2 
PRINTS