CPLM 1.0 - Compendium of Protein Lysine Modification
TagContent
CPLM ID CPLM-031388
UniProt Accession
Genbank Protein ID
Genbank Nucleotide ID
Protein Name
 Uncharacterized protein 
Protein Synonyms/Alias
 cDNA FLJ50912, highly similar to ATP-dependent RNA helicase DDX3Y (EC 3.6.1.-) 
Gene Name
 DDX3Y 
Gene Synonyms/Alias
  
Created Date
 July 27, 2013 
Organism
 Homo sapiens (Human) 
NCBI Taxa ID
 9606 
Lysine Modification
Position
Peptide
Type
References
203TRPTPVQKHAIPIIKubiquitination[1, 2, 3]
247DGPGEALKAVKENGRubiquitination[4]
259NGRYGRRKQYPISLVubiquitination[1, 2, 3, 5]
330VDMMERGKIGLDFCKubiquitination[2, 4, 6]
337KIGLDFCKYLVLDEAubiquitination[2, 3, 6]
368EQDTMPPKGVRHTMMubiquitination[2]
447LVFVETKKGADSLEDubiquitination[2]
486LHQFRSGKSPILVATubiquitination[2, 3]
543ATSFFNEKNMNITKDubiquitination[2, 4]
549EKNMNITKDLLDLLVubiquitination[4]
Reference
 [1] A proteome-wide, quantitative survey of in vivo ubiquitylation sites reveals widespread regulatory roles.
 Wagner SA, Beli P, Weinert BT, Nielsen ML, Cox J, Mann M, Choudhary C.
 Mol Cell Proteomics. 2011 Oct;10(10):M111.013284. [PMID: 21890473]
 [2] Refined preparation and use of anti-diglycine remnant (K-ε-GG) antibody enables routine quantification of 10,000s of ubiquitination sites in single proteomics experiments.
 Udeshi ND, Svinkina T, Mertins P, Kuhn E, Mani DR, Qiao JW, Carr SA.
 Mol Cell Proteomics. 2013 Mar;12(3):825-31. [PMID: 23266961]
 [3] Integrated proteomic analysis of post-translational modifications by serial enrichment.
 Mertins P, Qiao JW, Patel J, Udeshi ND, Clauser KR, Mani DR, Burgess MW, Gillette MA, Jaffe JD, Carr SA.
 Nat Methods. 2013 Jul;10(7):634-7. [PMID: 23749302]
 [4] Systematic and quantitative assessment of the ubiquitin-modified proteome.
 Kim W, Bennett EJ, Huttlin EL, Guo A, Li J, Possemato A, Sowa ME, Rad R, Rush J, Comb MJ, Harper JW, Gygi SP.
 Mol Cell. 2011 Oct 21;44(2):325-40. [PMID: 21906983]
 [5] Methods for quantification of in vivo changes in protein ubiquitination following proteasome and deubiquitinase inhibition.
 Udeshi ND, Mani DR, Eisenhaure T, Mertins P, Jaffe JD, Clauser KR, Hacohen N, Carr SA.
 Mol Cell Proteomics. 2012 May;11(5):148-59. [PMID: 22505724]
 [6] Global identification of modular cullin-RING ligase substrates.
 Emanuele MJ, Elia AE, Xu Q, Thoma CR, Izhar L, Leng Y, Guo A, Chen YN, Rush J, Hsu PW, Yen HC, Elledge SJ.
 Cell. 2011 Oct 14;147(2):459-74. [PMID: 21963094
Functional Description
  
Sequence Annotation
  
Keyword
 ATP-binding; Complete proteome; Helicase; Hydrolase; Nucleotide-binding; Reference proteome. 
Sequence Source
 UniProt (SWISSPROT/TrEMBL); GenBank; EMBL 
Protein Length
 657 AA 
Protein Sequence
MEIGRPRKDT WKLANLDLNS EKQSGGASTA SKGRYIPPHL RNREASKGFH DKDSSGWSCS 60
KDKDAYSSFG SRDSRGKPGY FSERGSGSRG RFDDRGRSDY DGIGNRERPG FGRFERSGHS 120
RWCDKSVEDD WSKPLPPSER LEQELFSGGN TGINFEKYDD IPVEATGSNC PPHIENFSDI 180
DMGEIIMGNI ELTRYTRPTP VQKHAIPIIK GKRDLMACAQ TGSGKTAAFL LPILSQIYTD 240
GPGEALKAVK ENGRYGRRKQ YPISLVLAPT RELAVQIYEE ARKFSYRSRV RPCVVYGGAD 300
IGQQIRDLER GCHLLVATPG RLVDMMERGK IGLDFCKYLV LDEADRMLDM GFEPQIRRIV 360
EQDTMPPKGV RHTMMFSATF PKEIQMLARD FLDEYIFLAV GRVGSTSENI TQKVVWVEDL 420
DKRSFLLDIL GATGSDSLTL VFVETKKGAD SLEDFLYHEG YACTSIHGDR SQRDREEALH 480
QFRSGKSPIL VATAVAARGL DISNVRHVIN FDLPSDIEEY VHRIGRTGRV GNLGLATSFF 540
NEKNMNITKD LLDLLVEAKQ EVPSWLENMA YEHHYKGGSR GRSKSNRFSG GFGARDYRQS 600
SGSSSSGFGA SRGSSSRSGG GGYGNSRGFG GGGYGGFYNS DGYGGNYNSQ GVDWWGN 657 
Gene Ontology
 GO:0005737; C:cytoplasm; IDA:HPA.
 GO:0005524; F:ATP binding; IEA:UniProtKB-KW.
 GO:0008026; F:ATP-dependent helicase activity; IEA:InterPro.
 GO:0003676; F:nucleic acid binding; IEA:InterPro. 
Interpro
 IPR011545; DNA/RNA_helicase_DEAD/DEAH_N.
 IPR014001; Helicase_ATP-bd.
 IPR001650; Helicase_C.
 IPR027417; P-loop_NTPase.
 IPR000629; RNA-helicase_DEAD-box_CS.
 IPR014014; RNA_helicase_DEAD_Q_motif. 
Pfam
 PF00270; DEAD
 PF00271; Helicase_C 
SMART
 SM00487; DEXDc
 SM00490; HELICc 
PROSITE
 PS00039; DEAD_ATP_HELICASE
 PS51192; HELICASE_ATP_BIND_1
 PS51194; HELICASE_CTER
 PS51195; Q_MOTIF 
PRINTS