CPLM 1.0 - Compendium of Protein Lysine Modification
TagContent
CPLM ID CPLM-005120
UniProt Accession
Genbank Protein ID
Genbank Nucleotide ID
Protein Name
 Insulin-degrading enzyme 
Protein Synonyms/Alias
 Insulin protease; Insulinase; Insulysin 
Gene Name
 Ide 
Gene Synonyms/Alias
 CG5517 
Created Date
 July 27, 2013 
Organism
 Drosophila melanogaster (Fruit fly) 
NCBI Taxa ID
 7227 
Lysine Modification
Position
Peptide
Type
References
281KVKIVPIKDIRSLTIacetylation[1]
Reference
 [1] Proteome-wide mapping of the Drosophila acetylome demonstrates a high degree of conservation of lysine acetylation.
 Weinert BT, Wagner SA, Horn H, Henriksen P, Liu WR, Olsen JV, Jensen LJ, Choudhary C.
 Sci Signal. 2011 Jul 26;4(183):ra48. [PMID: 21791702
Functional Description
 Can cleave insulin and TGF-alpha. 
Sequence Annotation
 ACT_SITE 84 84 Proton acceptor (By similarity).
 METAL 81 81 Zinc (By similarity).
 METAL 85 85 Zinc (By similarity).
 METAL 162 162 Zinc (By similarity).  
Keyword
 Complete proteome; Direct protein sequencing; Hydrolase; Metal-binding; Metalloprotease; Protease; Reference proteome; Zinc. 
Sequence Source
 UniProt (SWISSPROT/TrEMBL); GenBank; EMBL 
Protein Length
 990 AA 
Protein Sequence
MTIAESSQKS ATRKPDSMEP ILRLNNIEKS LQDTRDYRGL QLENGLKVLL ISDPNTDVSA 60
AALSVQVGHM SDPTNLPGLA HFCEHMLFLG TEKYPHENGY TTYLSQSGGS SNAATYPLMT 120
KYHFHVAPDK LDGALDRFAQ FFIAPLFTPS ATEREINAVN SEHEKNLPSD LWRIKQVNRH 180
LAKPDHAYSK FGSGNKTTLS EIPKSKNIDV RDELLKFHKQ WYSANIMCLA VIGKESLDEL 240
EGMVLEKFSE IENKNVKVPG WPRHPYAEER YGQKVKIVPI KDIRSLTISF TTDDLTQFYK 300
SGPDNYLTHL IGHEGKGSIL SELRRLGWCN DLMAGHQNTQ NGFGFFDIVV DLTQEGLEHV 360
DDIVKIVFQY LEMLRKEGPK KWIFDECVKL NEMRFRFKEK EQPENLVTHA VSSMQIFPLE 420
EVLIAPYLSN EWRPDLIKGL LDELVPSKSR IVIVSQSFEP DCDLAEPYYK TKYGITRVAK 480
DTVQSWENCE LNENLKLALP NSFIPTNFDI SDVPADAPKH PTIILDTPIL RVWHKQDNQF 540
NKPKACMTFD MSNPIAYLDP LNCNLNHMMV MLLKDQLNEY LYDAELASLK LSVMGKSCGI 600
DFTIRGFSDK QVVLLEKLLD HLFDFSIDEK RFDILKEEYV RSLKNFKAEQ PYQHSIYYLA 660
LLLTENAWAN MELLDAMELV TYDRVLNFAK EFFQRLHTEC FIFGNVTKQQ ATDIAGRVNT 720
RLEATNASKL PILARQMLKK REYKLLAGDS YLFEKENEFH KSSCAQLYLQ CGAQTDHTNI 780
MVNLVSQVLS EPCYDCLRTK EQLGYIVFSG VRKVNGANGI RIIVQSAKHP SYVEDRIENF 840
LQTYLQVIED MPLDEFERHK EALAVKKLEK PKTIFQQFSQ FYGEIAMQTY HFEREEAEVA 900
ILRKISKADF VDYFKKFIAK DGEERRVLSV HIVSQQTDEN ATSEAEPVEI TNMERHKPIS 960
DIVTFKSCKE LYPIALPFLD IKAKGARSKL 990 
Gene Ontology
 GO:0046872; F:metal ion binding; IEA:UniProtKB-KW.
 GO:0004222; F:metalloendopeptidase activity; IEA:InterPro.
 GO:0008340; P:determination of adult lifespan; IMP:FlyBase.
 GO:0045926; P:negative regulation of growth; IMP:FlyBase.
 GO:0006508; P:proteolysis; IEA:UniProtKB-KW.
 GO:0046662; P:regulation of oviposition; IMP:FlyBase.
 GO:0090062; P:regulation of trehalose metabolic process; IDA:FlyBase. 
Interpro
 IPR011249; Metalloenz_LuxS/M16.
 IPR011237; Pept_M16_dom.
 IPR011765; Pept_M16_N.
 IPR001431; Pept_M16_Zn_BS.
 IPR007863; Peptidase_M16_C. 
Pfam
 PF00675; Peptidase_M16
 PF05193; Peptidase_M16_C 
SMART
  
PROSITE
 PS00143; INSULINASE 
PRINTS