| Tag | Content |
|---|
| CPLM ID | CPLM-023453 |
UniProt Accession | |
Genbank Protein ID | |
Genbank Nucleotide ID | |
Protein Name | Nucleosome-remodeling factor subunit NURF301 |
Protein Synonyms/Alias | Enhancer of bithorax; Nucleosome-remodeling factor 215 kDa subunit; NURF-215 |
Gene Name | E(bx) |
Gene Synonyms/Alias | CG32346; CG7022; Nurf301 |
Created Date | July 27, 2013 |
Organism | Drosophila melanogaster (Fruit fly) |
NCBI Taxa ID | 7227 |
Lysine Modification | Position | Peptide | Type | References |
|---|
| 1167 | KKSYIGTKDVLDQTL | acetylation | [1] | | 1542 | GSRRVIVKNPDGTTR | acetylation | [1] |
|
Reference | [1] Proteome-wide mapping of the Drosophila acetylome demonstrates a high degree of conservation of lysine acetylation. Weinert BT, Wagner SA, Horn H, Henriksen P, Liu WR, Olsen JV, Jensen LJ, Choudhary C. Sci Signal. 2011 Jul 26;4(183):ra48. [ PMID: 21791702] |
Functional Description | Histone-binding component of NURF (nucleosome remodeling factor), a complex which catalyzes ATP-dependent nucleosome sliding and facilitates transcription of chromatin. Specifically recognizes H3 tails trimethylated on 'Lys-4' (H3K4me3), which mark transcription start sites of virtually all active genes. Required for homeotic gene expression, proper larval blood cell development, normal male X chromosome morphology, ecdysteroid signaling and metamorphosis. |
Sequence Annotation | DOMAIN 188 248 DDT.
DOMAIN 2573 2643 Bromo.
DNA_BIND 6 18 A.T hook.
ZN_FING 339 386 PHD-type 1.
ZN_FING 2481 2546 PHD-type 2.
REGION 1 121 Required for function in nucleosome
BINDING 2497 2497 Histone H3K4me3 (By similarity).
BINDING 2504 2504 Histone H3K4me3 (By similarity).
BINDING 2510 2510 Histone H3K4me3 (By similarity).
BINDING 2519 2519 Histone H3K4me3 (By similarity).
MOD_RES 40 40 Phosphoserine.
MOD_RES 52 52 Phosphoserine.
MOD_RES 55 55 Phosphoserine.
MOD_RES 59 59 Phosphoserine.
MOD_RES 62 62 Phosphoserine.
MOD_RES 1417 1417 Phosphoserine.
MOD_RES 1527 1527 Phosphothreonine.
MOD_RES 2395 2395 Phosphoserine.
MOD_RES 2398 2398 Phosphoserine.
MOD_RES 2403 2403 Phosphoserine. |
Keyword | Alternative splicing; Bromodomain; Chromatin regulator; Coiled coil; Complete proteome; Developmental protein; Direct protein sequencing; DNA-binding; Metal-binding; Nucleus; Phosphoprotein; Reference proteome; Repeat; Transcription; Transcription regulation; Zinc; Zinc-finger. |
Sequence Source | UniProt (SWISSPROT/TrEMBL); GenBank; EMBL |
Protein Length | 2669 AA |
Protein Sequence | MSGRGSRKRG RPPKTPNERA SGRFNYQLLK KPKYLSEGKS QPSTPSASRG ISPQSDEGSR 60
SSHNNHTNRS RGSAAKRGRG RKSAVQPNTS SYSGRKGYES EYHYGSDFGD SEEDKSDNED 120
DMLLTPSDDE SLEVANESES EFSVCSFNQN GVGRPPRPPS PEPVWLQEGR QYAALDLPDS 180
SEDLFIANTH VLRALSIYEV LRRFRHMVRL SPFRFEDLCA ALACEEQSAL LTEVHIMLLK 240
AILREEDAQG THFGPLDQKD TVNISLYLID SITWPEVLRS YVESDKTFDR NVFHILSHTE 300
YPYTGIDNRL EVLQFLSDQF LTSNSIRDVM LQEGPIHYDD HCRVCHRLGD LLCCETCPAV 360
YHLECVDPPM NDVPTEDWQC GLCRSHKVSG VVDCVLPQEK QGVLIRHDSL GVDRHGRKYW 420
FIARRIFIED QENFTCWYYS TTSKLKLLLS RLDAEELETR LHSQITERRD EIERQMKLTE 480
TLTNEHKHTK RSVIEIEQEA KNELLEKEVL DEDEKDGDAK SESQSIEGTK KQEECKMVTR 540
QKSNQLTNGT LHFKLGMEQG FKNYVNQYST NPIALNKPQR NEERDKRRHL SHKFSLTTAS 600
DFKWIGITMG TTDNMITTLR QTLINFESNI AASFLNINWV VNKKIWNAAV MNARRPSEFA 660
VVLLLFQASL KSVVFANVWH EQLGHTTLQR ITSAEREERK KLEKREKRER DDEEERNRLA 720
FNYIKYTLGL KHQVWKQKGE EYRVHGQWGW LWLSSSRRCG VRARRAQPLT HNRVYVHYTM 780
GEENDVNEII LVDPRTQRFM QQCESSNVDG QVCHYLPDQY KNVKVIEDVT EKIKGHIDVS 840
KALNAPGRTY YSKVARKSRL DDLLDRRLKL AEVEEQMASK IPSDMKPLLV SSQNNTANSK 900
QTFLEKRLLR LTEVQAKGGP ANVNLELVNS LAKQIQTVRL QFSQLNRFAK VFRCYTKECN 960
TNSNAVSQIT QNTCYSPLCL QKARAKKELL LLLRKAHTAG NGSKETVAAI LGAVKKPSIL 1020
EQKLTEGKRE STQVAVDDSE EGKPAESEAP LDLLQDWEHA RAHAVPFSDS LLTECILVDQ 1080
ECVTNTKIKQ EVNASSGCNT TPDSNTQDSD KIDYIESMDV CSNVEIESTE DSIVTGLNSG 1140
NAEDVDMTPG WRRKRNQKSK KSYIGTKDVL DQTLDKDIPL NKQNRRFPIT ARPVKRECVK 1200
KYERETFENG NERVYSTSSP RGRVYLLNDA AKLYEQAVKT EDKSTITKKP SYSRYPLISN 1260
FLTHKKKRSL LVLPRFELLK LARLGGKSST NGFHHAAKNN TIWQYQCSRP LFRTCWSYRT 1320
SNATSLSSLA LQLRILWSCL RWDDMIAKPP STDGKHQVTT DTEIVTLELL KLRHSGRYGE 1380
KTSYLRRKVV IPLEMPKTVR EVTSIRSGLR KRKRAESPQP TEPQITEEWV DEDKLELWEI 1440
KFMGEKQEKA RLSAVTRSVA SRQLEASGSN GSNTSTNGAL GVAGRVQLAP KLSEDVKEKM 1500
EQQLKLQRAV HQQRKLVATG EITRSVTPVK GQVIGSRRVI VKNPDGTTRI IQQAVTQVSR 1560
TGGANTAAAA ASPTVGGSTS TQSNPSTSTP HKVQIIRGPD GKVSVRGLNP GQQLVQMPDG 1620
KLHVLTTTTS SNSAGQGNKM KVPIKPASTS SSPAISSAQT TTNPVTPVIK QIAVKHVTKN 1680
SATQSIASSS RVALPLAQIK NKLLLAQQQQ QSTSSSPATS SSPVQKIVSK VVNTSTSGQT 1740
LQQVFVQSGS KLVVGQNAQG QKVIISTSAA QQQGTSPVQQ QQLVQSQPIQ QSPQQISMTQ 1800
VGNQPTQKVI QQIVNTSNVQ QQIVVGGQRI ILSPGQTIVT QRNVPQSQAL QMVQQQIQTQ 1860
QQQQQHHVVQ PQQQFVVQSN QIVQSSPSAQ TKLVKQLVVQ QQSQQTIEEK TQITTTDSNE 1920
TGTQQVLVPN STLAQQLAQG KLQVATVNGQ QVIVKPLGNN QAQIVAHIKH QGDGNAHIVT 1980
SNSATAVPQA NPQTSPVKQQ ALPPQSPQQV VVQQQQIHQQ SPTNFESGVT PITQQPVLTQ 2040
AVQAPAQQQA LSVEESLLQN QPPGTVIKCV TAQVLQTEHG PRIVLQGLVG NDFTAQQLQL 2100
VQTQVKQQLM KAQESNGKLG VLGPTKIYLA VQPENAVQSQ PPPLTPVHQS AAHQQTNNIE 2160
IDADTLATTY EANSTIKDIA INNGDDQENS KCAETENSNI TTNESFAGTS SLLEGSEHDE 2220
PTNLAGLDIS ETDLENKQNE SFVVTRGYIQ KSISNALKQG NLSPELEEKL VCMQKQQENA 2280
NSTNEWETCS RGSVNEEALT PSRQTDDTEW KIRTSLRRPN AMTTSSQFNR ILKKNRSKND 2340
EVAELGEQKQ SQLERHKELL KKNILRKRSL LERNLQSEIH EDVKTKVQRH VRPLSNASPD 2400
EQSENERSGE PNLDFKRTEV QNPRHGAGRP KKLTRKKEKL YCICRTPYDD TKFYVGCDLC 2460
SNWFHGDCVS ITEEASKKLS EFICIDCKRA RETQQLYCSC RQPYDESQFY ICCDKCQDWF 2520
HGRCVGILQS EAEFIDEYVC PECQRKNDAN AANMKKLTSN DVEELKNLIK QMQLHKSAWP 2580
FMEPVDPKEA PDYYKVIKEP MDLKRMEIKL ESNTYTKLSE FIGDMTKIFD NCRYYNPKES 2640
SFYKCAEALE SYFVQKIKNF RENVFDQRT 2669 |
Gene Ontology | GO:0016589; C:NURF complex; IDA:FlyBase. GO:0003677; F:DNA binding; IEA:UniProtKB-KW. GO:0008270; F:zinc ion binding; IEA:InterPro. GO:0048813; P:dendrite morphogenesis; IMP:FlyBase. GO:0035076; P:ecdysone receptor-mediated signaling pathway; IGI:FlyBase. GO:0030097; P:hemopoiesis; IMP:FlyBase. GO:0046331; P:lateral inhibition; IMP:FlyBase. GO:0007095; P:mitotic G2 DNA damage checkpoint; IGI:FlyBase. GO:0042766; P:nucleosome mobilization; IDA:FlyBase. GO:0045747; P:positive regulation of Notch signaling pathway; IMP:FlyBase. GO:0035073; P:pupariation; IMP:FlyBase. GO:0006357; P:regulation of transcription from RNA polymerase II promoter; IMP:FlyBase. GO:0006351; P:transcription, DNA-dependent; IDA:FlyBase. |
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Pfam | |
SMART | |
PROSITE | |
PRINTS | |