CPLM-023134

Genbank Nucleotide ID

Ras-related protein Rab-21

Protein Synonyms/Alias

Homo sapiens (Human)

Position	Peptide	Type	References
109	TDEDSFQKVKNWVKE	ubiquitination	[1]
115	QKVKNWVKELRKMLG	acetylation	[2]
115	QKVKNWVKELRKMLG	ubiquitination	[3]
138	GNKIDLEKERHVSIQ	ubiquitination	[4, 5]
157	YAESVGAKHYHTSAK	ubiquitination	[1, 4, 5, 6]
164	KHYHTSAKQNKGIEE	ubiquitination	[4]

[1] Proteome-wide identification of ubiquitylation sites by conjugation of engineered lysine-less ubiquitin.
Oshikawa K, Matsumoto M, Oyamada K, Nakayama KI.
J Proteome Res. 2012 Feb 3;11(2):796-807. [PMID: 22053931]
[2] Lysine acetylation targets protein complexes and co-regulates major cellular functions.
Choudhary C, Kumar C, Gnad F, Nielsen ML, Rehman M, Walther TC, Olsen JV, Mann M.
Science. 2009 Aug 14;325(5942):834-40. [PMID: 19608861]
[3] Systems-wide analysis of ubiquitylation dynamics reveals a key role for PAF15 ubiquitylation in DNA-damage bypass.
Povlsen LK, Beli P, Wagner SA, Poulsen SL, Sylvestersen KB, Poulsen JW, Nielsen ML, Bekker-Jensen S, Mailand N, Choudhary C.
Nat Cell Biol. 2012 Oct;14(10):1089-98. [PMID: 23000965]
[4] Refined preparation and use of anti-diglycine remnant (K-ε-GG) antibody enables routine quantification of 10,000s of ubiquitination sites in single proteomics experiments.
Udeshi ND, Svinkina T, Mertins P, Kuhn E, Mani DR, Qiao JW, Carr SA.
Mol Cell Proteomics. 2013 Mar;12(3):825-31. [PMID: 23266961]
[5] Integrated proteomic analysis of post-translational modifications by serial enrichment.
Mertins P, Qiao JW, Patel J, Udeshi ND, Clauser KR, Mani DR, Burgess MW, Gillette MA, Jaffe JD, Carr SA.
Nat Methods. 2013 Jul;10(7):634-7. [PMID: 23749302]
[6] Landscape of the PARKIN-dependent ubiquitylome in response to mitochondrial depolarization.
Sarraf SA, Raman M, Guarani-Pereira V, Sowa ME, Huttlin EL, Gygi SP, Harper JW.
Nature. 2013 Apr 18;496(7445):372-6. [PMID: 23503661]

Functional Description

Regulates integrin internalization and recycling, but does not influence the traffic of endosomally translocated receptors in general. As a result, may regulate cell adhesion and migration (By similarity). During the mitosis of adherent cells, controls the endosomal trafficking of integrins which is required for the successful completion of cytokinesis.

NP_BIND 26 34 GTP.
NP_BIND 74 78 GTP (By similarity).
NP_BIND 132 135 GTP.
NP_BIND 162 164 GTP.
MOTIF 48 56 Effector region (By similarity).
MOD_RES 2 2 N-acetylalanine.
MOD_RES 222 222 Cysteine methyl ester (Potential).
LIPID 221 221 S-geranylgeranyl cysteine (By
LIPID 222 222 S-geranylgeranyl cysteine (By

3D-structure; Acetylation; Complete proteome; Cytoplasmic vesicle; Direct protein sequencing; Endoplasmic reticulum; Endosome; Golgi apparatus; GTP-binding; Lipoprotein; Membrane; Methylation; Nucleotide-binding; Prenylation; Protein transport; Reference proteome; Transport.

UniProt (SWISSPROT/TrEMBL); GenBank; EMBL

GO:0032154; C:cleavage furrow; IEA:UniProtKB-SubCell.
GO:0030659; C:cytoplasmic vesicle membrane; IEA:UniProtKB-SubCell.
GO:0031901; C:early endosome membrane; IEA:UniProtKB-SubCell.
GO:0005789; C:endoplasmic reticulum membrane; IEA:UniProtKB-SubCell.
GO:0005768; C:endosome; IDA:LIFEdb.
GO:0000139; C:Golgi membrane; IEA:UniProtKB-SubCell.
GO:0019003; F:GDP binding; IDA:UniProtKB.
GO:0005525; F:GTP binding; IDA:UniProtKB.
GO:0003924; F:GTPase activity; IDA:UniProtKB.
GO:0015031; P:protein transport; IEA:UniProtKB-KW.
GO:0007264; P:small GTPase mediated signal transduction; IEA:InterPro.

IPR027417; P-loop_NTPase.
IPR005225; Small_GTP-bd_dom.
IPR001806; Small_GTPase.
IPR003579; Small_GTPase_Rab_type.

PR00449; RASTRNSFRMNG.