CPLM-020146

Genbank Nucleotide ID

COP9 signalosome complex subunit 4

Protein Synonyms/Alias

SGN4; Signalosome subunit 4; JAB1-containing signalosome subunit 4

Homo sapiens (Human)

Position	Peptide	Type	References
20	MNSSGSHKDLAGKYR	ubiquitination	[1, 2]
25	SHKDLAGKYRQILEK	acetylation	[3]
137	IPLETGQKQYNVDYK	ubiquitination	[4]
150	YKLETYLKIARLYLE	ubiquitination	[4, 5]
200	RVLDYRRKFIEAAQR	ubiquitination	[5]
214	RYNELSYKTIVHESE	ubiquitination	[5]
227	SERLEALKHALHCTI	ubiquitination	[5]
251	RMLATLFKDERCQQL	ubiquitination	[5]
266	AAYGILEKMYLDRII	ubiquitination	[5]
290	AMLMPHQKATTADGS	ubiquitination	[5]
334	LLEIPAAKAEKIASQ	ubiquitination	[6]
337	IPAAKAEKIASQMIT	ubiquitination	[5]
372	EALPTWDKQIQSLCF	ubiquitination	[5]

[1] A proteome-wide, quantitative survey of in vivo ubiquitylation sites reveals widespread regulatory roles.
Wagner SA, Beli P, Weinert BT, Nielsen ML, Cox J, Mann M, Choudhary C.
Mol Cell Proteomics. 2011 Oct;10(10):M111.013284. [PMID: 21890473]
[2] hCKSAAP_UbSite: improved prediction of human ubiquitination sites by exploiting amino acid pattern and properties.
Chen Z, Zhou Y, Song J, Zhang Z.
Biochim Biophys Acta. 2013 Aug;1834(8):1461-7. [PMID: 23603789]
[3] Lysine acetylation targets protein complexes and co-regulates major cellular functions.
Choudhary C, Kumar C, Gnad F, Nielsen ML, Rehman M, Walther TC, Olsen JV, Mann M.
Science. 2009 Aug 14;325(5942):834-40. [PMID: 19608861]
[4] Systematic and quantitative assessment of the ubiquitin-modified proteome.
Kim W, Bennett EJ, Huttlin EL, Guo A, Li J, Possemato A, Sowa ME, Rad R, Rush J, Comb MJ, Harper JW, Gygi SP.
Mol Cell. 2011 Oct 21;44(2):325-40. [PMID: 21906983]
[5] Refined preparation and use of anti-diglycine remnant (K-ε-GG) antibody enables routine quantification of 10,000s of ubiquitination sites in single proteomics experiments.
Udeshi ND, Svinkina T, Mertins P, Kuhn E, Mani DR, Qiao JW, Carr SA.
Mol Cell Proteomics. 2013 Mar;12(3):825-31. [PMID: 23266961]
[6] Landscape of the PARKIN-dependent ubiquitylome in response to mitochondrial depolarization.
Sarraf SA, Raman M, Guarani-Pereira V, Sowa ME, Huttlin EL, Gygi SP, Harper JW.
Nature. 2013 Apr 18;496(7445):372-6. [PMID: 23503661]

Functional Description

Component of the COP9 signalosome complex (CSN), a complex involved in various cellular and developmental processes. The CSN complex is an essential regulator of the ubiquitin (Ubl) conjugation pathway by mediating the deneddylation of the cullin subunits of SCF-type E3 ligase complexes, leading to decrease the Ubl ligase activity of SCF-type complexes such as SCF, CSA or DDB2. The complex is also involved in phosphorylation of p53/TP53, c-jun/JUN, IkappaBalpha/NFKBIA, ITPK1 and IRF8/ICSBP, possibly via its association with CK2 and PKD kinases. CSN-dependent phosphorylation of TP53 and JUN promotes and protects degradation by the Ubl system, respectively.

DOMAIN 201 363 PCI.
MOD_RES 2 2 N-acetylalanine.
MOD_RES 25 25 N6-acetyllysine.

Acetylation; Alternative splicing; Complete proteome; Cytoplasm; Direct protein sequencing; Nucleus; Reference proteome; Signalosome.

UniProt (SWISSPROT/TrEMBL); GenBank; EMBL

GO:0005737; C:cytoplasm; IEA:UniProtKB-SubCell.
GO:0008180; C:signalosome; IDA:UniProtKB.
GO:0010388; P:cullin deneddylation; IDA:UniProtKB.

IPR000717; PCI_dom.
IPR011991; WHTH_DNA-bd_dom.