CPLM 1.0 - Compendium of Protein Lysine Modification
TagContent
CPLM ID CPLM-001664
UniProt Accession
Genbank Protein ID
Genbank Nucleotide ID
Protein Name
 Tetratricopeptide repeat protein 4 
Protein Synonyms/Alias
 TPR repeat protein 4 
Gene Name
 TTC4 
Gene Synonyms/Alias
 My044 
Created Date
 July 27, 2013 
Organism
 Homo sapiens (Human) 
NCBI Taxa ID
 9606 
Lysine Modification
Position
Peptide
Type
References
37FHEDQWEKEFEKVPLubiquitination[1, 2]
41QWEKEFEKVPLFMSRubiquitination[3]
227EALLQAIKARNIRLSacetylation[4]
227EALLQAIKARNIRLSubiquitination[3, 5, 6, 7, 8]
343ELYRVPAKSTLLQVLubiquitination[3]
Reference
 [1] A proteome-wide, quantitative survey of in vivo ubiquitylation sites reveals widespread regulatory roles.
 Wagner SA, Beli P, Weinert BT, Nielsen ML, Cox J, Mann M, Choudhary C.
 Mol Cell Proteomics. 2011 Oct;10(10):M111.013284. [PMID: 21890473]
 [2] hCKSAAP_UbSite: improved prediction of human ubiquitination sites by exploiting amino acid pattern and properties.
 Chen Z, Zhou Y, Song J, Zhang Z.
 Biochim Biophys Acta. 2013 Aug;1834(8):1461-7. [PMID: 23603789]
 [3] Refined preparation and use of anti-diglycine remnant (K-ε-GG) antibody enables routine quantification of 10,000s of ubiquitination sites in single proteomics experiments.
 Udeshi ND, Svinkina T, Mertins P, Kuhn E, Mani DR, Qiao JW, Carr SA.
 Mol Cell Proteomics. 2013 Mar;12(3):825-31. [PMID: 23266961]
 [4] Lysine acetylation targets protein complexes and co-regulates major cellular functions.
 Choudhary C, Kumar C, Gnad F, Nielsen ML, Rehman M, Walther TC, Olsen JV, Mann M.
 Science. 2009 Aug 14;325(5942):834-40. [PMID: 19608861]
 [5] Systematic and quantitative assessment of the ubiquitin-modified proteome.
 Kim W, Bennett EJ, Huttlin EL, Guo A, Li J, Possemato A, Sowa ME, Rad R, Rush J, Comb MJ, Harper JW, Gygi SP.
 Mol Cell. 2011 Oct 21;44(2):325-40. [PMID: 21906983]
 [6] Global identification of modular cullin-RING ligase substrates.
 Emanuele MJ, Elia AE, Xu Q, Thoma CR, Izhar L, Leng Y, Guo A, Chen YN, Rush J, Hsu PW, Yen HC, Elledge SJ.
 Cell. 2011 Oct 14;147(2):459-74. [PMID: 21963094]
 [7] Methods for quantification of in vivo changes in protein ubiquitination following proteasome and deubiquitinase inhibition.
 Udeshi ND, Mani DR, Eisenhaure T, Mertins P, Jaffe JD, Clauser KR, Hacohen N, Carr SA.
 Mol Cell Proteomics. 2012 May;11(5):148-59. [PMID: 22505724]
 [8] Integrated proteomic analysis of post-translational modifications by serial enrichment.
 Mertins P, Qiao JW, Patel J, Udeshi ND, Clauser KR, Mani DR, Burgess MW, Gillette MA, Jaffe JD, Carr SA.
 Nat Methods. 2013 Jul;10(7):634-7. [PMID: 23749302
Functional Description
  
Sequence Annotation
 REPEAT 79 112 TPR 1.
 REPEAT 117 150 TPR 2.
 REPEAT 151 184 TPR 3.
 MOD_RES 1 1 N-acetylmethionine.
 MOD_RES 243 243 Phosphoserine.  
Keyword
 Acetylation; Complete proteome; Phosphoprotein; Polymorphism; Reference proteome; Repeat; TPR repeat. 
Sequence Source
 UniProt (SWISSPROT/TrEMBL); GenBank; EMBL 
Protein Length
 387 AA 
Protein Sequence
MEQPGQDPTS DDVMDSFLEK FQSQPYRGGF HEDQWEKEFE KVPLFMSRAP SEIDPRENPD 60
LACLQSIIFD EERSPEEQAK TYKDEGNDYF KEKDYKKAVI SYTEGLKKKC ADPDLNAVLY 120
TNRAAAQYYL GNFRSALNDV TAARKLKPCH LKAIIRGALC HLELKHFAEA VNWCDEGLQI 180
DAKEKKLLEM RAKADKLKRI EQRDVRKANL KEKKERNQNE ALLQAIKARN IRLSEAACED 240
EDSASEGLGE LFLDGLSTEN PHGARLSLDG QGRLSWPVLF LYPEYAQSDF ISAFHEDSRF 300
IDHLMVMFGE TPSWDLEQKY CPDNLEVYFE DEDRAELYRV PAKSTLLQVL QHQRYFVKAL 360
TPAFLVCVGS SPFCKNFLRG RKVYQIR 387 
Gene Ontology
  
Interpro
 IPR013026; TPR-contain_dom.
 IPR011990; TPR-like_helical.
 IPR019734; TPR_repeat. 
Pfam
  
SMART
 SM00028; TPR 
PROSITE
 PS50005; TPR
 PS50293; TPR_REGION 
PRINTS