UniProt Accession

 BAG3_HUMAN; O95817; A8K5L8; Q3B763; Q9NT20; Q9P120 

Genbank Protein ID

 AF095193; AF127139; AF071218; AK291333; AL137582; BC006418; BC014656; BC107786 

Genbank Nucleotide ID

 AAD16122.2; AAF26839.1; AAF69592.2; BAF84022.1; CAB70824.1; AAH06418.1; AAH14656.1; AAI07787.1 

Protein Name

 BAG family molecular chaperone regulator 3 

Protein Synonyms/Alias

 BAG-3; Bcl-2-associated athanogene 3; Bcl-2-binding protein Bis; Docking protein CAIR-1 

Gene Name

 BAG3 

Gene Synonyms/Alias

 BIS 

Created Date

 July 27, 2013 

Organism

 Homo sapiens (Human) 

NCBI Taxa ID

 9606 

Lysine Modification

Position	Peptide	Type	References
60	RVPSEGPKETPSSAN	ubiquitination	[1]
450	EGKKTDKKYLMIEEY	ubiquitination	[2, 3, 4]

Reference

 [1] Global identification of modular cullin-RING ligase substrates.
 Emanuele MJ, Elia AE, Xu Q, Thoma CR, Izhar L, Leng Y, Guo A, Chen YN, Rush J, Hsu PW, Yen HC, Elledge SJ.
 Cell. 2011 Oct 14;147(2):459-74. [PMID: 21963094]
 [2] A proteome-wide, quantitative survey of in vivo ubiquitylation sites reveals widespread regulatory roles.
 Wagner SA, Beli P, Weinert BT, Nielsen ML, Cox J, Mann M, Choudhary C.
 Mol Cell Proteomics. 2011 Oct;10(10):M111.013284. [PMID: 21890473]
 [3] Systems-wide analysis of ubiquitylation dynamics reveals a key role for PAF15 ubiquitylation in DNA-damage bypass.
 Povlsen LK, Beli P, Wagner SA, Poulsen SL, Sylvestersen KB, Poulsen JW, Nielsen ML, Bekker-Jensen S, Mailand N, Choudhary C.
 Nat Cell Biol. 2012 Oct;14(10):1089-98. [PMID: 23000965]
 [4] hCKSAAP_UbSite: improved prediction of human ubiquitination sites by exploiting amino acid pattern and properties.
 Chen Z, Zhou Y, Song J, Zhang Z.
 Biochim Biophys Acta. 2013 Aug;1834(8):1461-7. [PMID: 23603789] 

Functional Description

 Inhibits the chaperone activity of HSP70/HSC70 by promoting substrate release. Has anti-apoptotic activity. 

Sequence Annotation

 DOMAIN 20 54 WW 1.
 DOMAIN 124 154 WW 2.
 DOMAIN 421 498 BAG.
 MOD_RES 2 2 N-acetylserine.
 MOD_RES 198 198 Phosphoserine.
 MOD_RES 274 274 Phosphoserine.
 MOD_RES 275 275 Phosphoserine.
 MOD_RES 279 279 Phosphoserine.
 MOD_RES 285 285 Phosphothreonine.
 MOD_RES 289 289 Phosphoserine.
 MOD_RES 291 291 Phosphoserine.
 MOD_RES 377 377 Phosphoserine.
 MOD_RES 385 385 Phosphoserine (By similarity).
 MOD_RES 386 386 Phosphoserine.
 MOD_RES 406 406 Phosphothreonine.  

Keyword

 Acetylation; Apoptosis; Cardiomyopathy; Chaperone; Complete proteome; Disease mutation; Myofibrillar myopathy; Phosphoprotein; Polymorphism; Reference proteome; Repeat. 

Sequence Source

 UniProt (SWISSPROT/TrEMBL); GenBank; EMBL 

Protein Length

 575 AA 

Protein Sequence

Gene Ontology

 GO:0005829; C:cytosol; IDA:MGI.
 GO:0043005; C:neuron projection; IEA:Compara.
 GO:0005886; C:plasma membrane; IDA:HPA.
 GO:0007420; P:brain development; IEA:Compara.
 GO:0008625; P:extrinsic apoptotic signaling pathway via death domain receptors; IDA:MGI.
 GO:0043066; P:negative regulation of apoptotic process; NAS:UniProtKB.
 GO:0006457; P:protein folding; NAS:UniProtKB.
 GO:0021510; P:spinal cord development; IEA:Compara. 

Interpro

 IPR003103; BAG_domain.
 IPR001202; WW_dom. 

Pfam

 PF02179; BAG
 PF00397; WW 

SMART

 SM00264; BAG
 SM00456; WW 

PROSITE

 PS51035; BAG
 PS01159; WW_DOMAIN_1
 PS50020; WW_DOMAIN_2 

PRINTS