CPLM 1.0 - Compendium of Protein Lysine Modification
TagContent
CPLM ID CPLM-005912
UniProt Accession
Genbank Protein ID
Genbank Nucleotide ID
Protein Name
 ATP-dependent helicase NAM7 
Protein Synonyms/Alias
 Nonsense-mediated mRNA decay protein 1; Nuclear accommodation of mitochondria 7 protein; Up-frameshift suppressor 1 
Gene Name
 NAM7 
Gene Synonyms/Alias
 IFS2; MOF4; UPF1; YMR080C; YM9582.05C 
Created Date
 July 27, 2013 
Organism
 Saccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast) 
NCBI Taxa ID
 559292 
Lysine Modification
Position
Peptide
Type
References
471AVDHLAAKLRDLGLKacetylation[1]
722KLFRDGVKPEQIGVIubiquitination[2]
926FKSAFSQKQNRNEIDubiquitination[2]
Reference
 [1] Proteome-wide analysis of lysine acetylation suggests its broad regulatory scope in Saccharomyces cerevisiae.
 Henriksen P, Wagner SA, Weinert BT, Sharma S, Bacinskaja G, Rehman M, Juffer AH, Walther TC, Lisby M, Choudhary C.
 Mol Cell Proteomics. 2012 Nov;11(11):1510-22. [PMID: 22865919]
 [2] Global analysis of phosphorylation and ubiquitylation cross-talk in protein degradation.
 Swaney DL, Beltrao P, Starita L, Guo A, Rush J, Fields S, Krogan NJ, Villén J.
 Nat Methods. 2013 Jul;10(7):676-82. [PMID: 23749301] 
Functional Description
 Probable helicase involved in mitochondrial functions. Required for rapid turnover of mRNAs containing a premature translational termination codon. 
Sequence Annotation
 ZN_FING 61 208 UPF1-type.
 NP_BIND 430 437 ATP (By similarity).
 MOD_RES 56 56 Phosphoserine.
 MOD_RES 869 869 Phosphoserine.  
Keyword
 3D-structure; ATP-binding; Complete proteome; Cytoplasm; Helicase; Hydrolase; Metal-binding; Nonsense-mediated mRNA decay; Nucleotide-binding; Nucleus; Phosphoprotein; Reference proteome; Zinc; Zinc-finger. 
Sequence Source
 UniProt (SWISSPROT/TrEMBL); GenBank; EMBL 
Protein Length
 971 AA 
Protein Sequence
MVGSGSHTPY DISNSPSDVN VQPATQLNST LVEDDDVDNQ LFEEAQVTET GFRSPSASDN 60
SCAYCGIDSA KCVIKCNSCK KWFCNTKNGT SSSHIVNHLV LSHHNVVSLH PDSDLGDTVL 120
ECYNCGRKNV FLLGFVSAKS EAVVVLLCRI PCAQTKNANW DTDQWQPLIE DRQLLSWVAE 180
QPTEEEKLKA RLITPSQISK LEAKWRSNKD ATINDIDAPE EQEAIPPLLL RYQDAYEYQR 240
SYGPLIKLEA DYDKQLKESQ ALEHISVSWS LALNNRHLAS FTLSTFESNE LKVAIGDEMI 300
LWYSGMQHPD WEGRGYIVRL PNSFQDTFTL ELKPSKTPPP THLTTGFTAE FIWKGTSYDR 360
MQDALKKFAI DKKSISGYLY YKILGHQVVD ISFDVPLPKE FSIPNFAQLN SSQSNAVSHV 420
LQRPLSLIQG PPGTGKTVTS ATIVYHLSKI HKDRILVCAP SNVAVDHLAA KLRDLGLKVV 480
RLTAKSREDV ESSVSNLALH NLVGRGAKGE LKNLLKLKDE VGELSASDTK RFVKLVRKTE 540
AEILNKADVV CCTCVGAGDK RLDTKFRTVL IDESTQASEP ECLIPIVKGA KQVILVGDHQ 600
QLGPVILERK AADAGLKQSL FERLISLGHV PIRLEVQYRM NPYLSEFPSN MFYEGSLQNG 660
VTIEQRTVPN SKFPWPIRGI PMMFWANYGR EEISANGTSF LNRIEAMNCE RIITKLFRDG 720
VKPEQIGVIT PYEGQRAYIL QYMQMNGSLD KDLYIKVEVA SVDAFQGREK DYIILSCVRA 780
NEQQAIGFLR DPRRLNVGLT RAKYGLVILG NPRSLARNTL WNHLLIHFRE KGCLVEGTLD 840
NLQLCTVQLV RPQPRKTERP MNAQFNVESE MGDFPKFQDF DAQSMVSFSG QIGDFGNAFV 900
DNTELSSYIN NEYWNFENFK SAFSQKQNRN EIDDRNLYQE EASHLNSNFA RELQREEQKH 960
ELSKDFSNLG I 971 
Gene Ontology
 GO:0005737; C:cytoplasm; IDA:SGD.
 GO:0005634; C:nucleus; IEA:UniProtKB-SubCell.
 GO:0005844; C:polysome; TAS:SGD.
 GO:0005524; F:ATP binding; IEA:UniProtKB-KW.
 GO:0004004; F:ATP-dependent RNA helicase activity; TAS:SGD.
 GO:0003677; F:DNA binding; IEA:InterPro.
 GO:0003676; F:nucleic acid binding; TAS:SGD.
 GO:0008270; F:zinc ion binding; IEA:InterPro.
 GO:0030466; P:chromatin silencing at silent mating-type cassette; IGI:SGD.
 GO:0008298; P:intracellular mRNA localization; IMP:SGD.
 GO:0070478; P:nuclear-transcribed mRNA catabolic process, 3'-5' exonucleolytic nonsense-mediated decay; IMP:SGD.
 GO:0016567; P:protein ubiquitination; IMP:SGD.
 GO:0006449; P:regulation of translational termination; TAS:SGD. 
Interpro
 IPR003593; AAA+_ATPase.
 IPR014001; Helicase_ATP-bd.
 IPR027417; P-loop_NTPase.
 IPR018999; RNA-helicase_UPF1_UPF2-interct. 
Pfam
 PF09416; UPF1_Zn_bind 
SMART
 SM00382; AAA
 SM00487; DEXDc 
PROSITE
  
PRINTS