UniProt Accession

 PKHO1_MOUSE; Q9JIY0; Q9CXH2 

Genbank Protein ID

 AF168675; AK014374; AK170025; AK170922; BC117003; BC125441 

Genbank Nucleotide ID

 AAF89643.1; BAB29306.1; BAE41517.1; BAE42115.1; AAI17004.1; AAI25442.1 

Protein Name

 Pleckstrin homology domain-containing family O member 1 

Protein Synonyms/Alias

 PH domain-containing family O member 1 

Gene Name

 Plekho1 

Gene Synonyms/Alias

  

Created Date

 July 27, 2013 

Organism

 Mus musculus (Mouse) 

NCBI Taxa ID

 10090 

Lysine Modification

Position	Peptide	Type	References
314	IQDLVARKLEKTQEL	ubiquitination	[1]

Reference

 [1] Proteomic analyses reveal divergent ubiquitylation site patterns in murine tissues.
 Wagner SA, Beli P, Weinert BT, Schölz C, Kelstrup CD, Young C, Nielsen ML, Olsen JV, Brakebusch C, Choudhary C.
 Mol Cell Proteomics. 2012 Dec;11(12):1578-85. [PMID: 22790023] 

Functional Description

 Plays a role in the regulation of the actin cytoskeleton through its interactions with actin capping protein (CP). May function to target CK2 to the plasma membrane thereby serving as an adapter to facilitate the phosphorylation of CP by protein kinase 2 (CK2). Appears to target ATM to the plasma membrane. Appears to also inhibit tumor cell growth by inhibiting AKT- mediated cell-survival. Also implicated in PI3K-regulated muscle differentiation, the regulation of AP-1 activity (plasma membrane bound AP-1 regulator that translocates to the nucleus) and the promotion of apoptosis induced by tumor necrosis factor TNF. When bound to PKB, it inhibits it probably by decreasing PKB level of phosphorylation (By similarity). 

Sequence Annotation

 DOMAIN 20 131 PH.
 REGION 132 192 Interaction with capping proteins (CPs)
 REGION 135 307 Interaction with ATM, CKIP, IFP35 and NMI
 REGION 307 408 Negative regulator of AP-1 activity (By
 BINDING 154 154 Capping protein (By similarity).
 BINDING 156 156 Capping protein (By similarity).
 MOD_RES 220 220 Phosphoserine.
 MOD_RES 226 226 Phosphoserine.
 MOD_RES 270 270 Phosphoserine.
 MOD_RES 341 341 Phosphoserine (By similarity).  

Keyword

 Complete proteome; Cytoplasm; Membrane; Nucleus; Phosphoprotein; Reference proteome; Tumor suppressor. 

Sequence Source

 UniProt (SWISSPROT/TrEMBL); GenBank; EMBL 

Protein Length

 408 AA 

Protein Sequence

Gene Ontology

 GO:0005737; C:cytoplasm; IEA:UniProtKB-SubCell.
 GO:0016020; C:membrane; IEA:UniProtKB-SubCell.
 GO:0005634; C:nucleus; IEA:UniProtKB-SubCell.
 GO:0005543; F:phospholipid binding; IEA:InterPro. 

Interpro

 IPR011993; PH_like_dom.
 IPR001849; Pleckstrin_homology. 

Pfam

 PF00169; PH 

SMART

 SM00233; PH 

PROSITE

 PS50003; PH_DOMAIN 

PRINTS