UniProt Accession

 VPS25_HUMAN; Q9BRG1; B2R581 

Genbank Protein ID

 AB014763; AK312092; CH471152; BC006282 

Genbank Nucleotide ID

 BAB87804.1; BAG35028.1; EAW60879.1; AAH06282.1 

Protein Name

 Vacuolar protein-sorting-associated protein 25 

Protein Synonyms/Alias

 hVps25; Dermal papilla-derived protein 9; ELL-associated protein of 20 kDa; ESCRT-II complex subunit VPS25 

Gene Name

 VPS25 

Gene Synonyms/Alias

 DERP9; EAP20 

Created Date

 July 27, 2013 

Organism

 Homo sapiens (Human) 

NCBI Taxa ID

 9606 

Lysine Modification

Position	Peptide	Type	References
64	SPLFNNVKLQRKLPV	ubiquitination	[1, 2]
84	VLEELRKKGNLEWLD	ubiquitination	[2]
92	GNLEWLDKSKSSFLI	ubiquitination	[2, 3, 4]
94	LEWLDKSKSSFLIMW	ubiquitination	[2]
109	RRPEEWGKLIYQWVS	ubiquitination	[5]
161	QALQQEHKAEIITVS	ubiquitination	[1]

Reference

 [1] Systematic and quantitative assessment of the ubiquitin-modified proteome.
 Kim W, Bennett EJ, Huttlin EL, Guo A, Li J, Possemato A, Sowa ME, Rad R, Rush J, Comb MJ, Harper JW, Gygi SP.
 Mol Cell. 2011 Oct 21;44(2):325-40. [PMID: 21906983]
 [2] Refined preparation and use of anti-diglycine remnant (K-ε-GG) antibody enables routine quantification of 10,000s of ubiquitination sites in single proteomics experiments.
 Udeshi ND, Svinkina T, Mertins P, Kuhn E, Mani DR, Qiao JW, Carr SA.
 Mol Cell Proteomics. 2013 Mar;12(3):825-31. [PMID: 23266961]
 [3] A proteome-wide, quantitative survey of in vivo ubiquitylation sites reveals widespread regulatory roles.
 Wagner SA, Beli P, Weinert BT, Nielsen ML, Cox J, Mann M, Choudhary C.
 Mol Cell Proteomics. 2011 Oct;10(10):M111.013284. [PMID: 21890473]
 [4] hCKSAAP_UbSite: improved prediction of human ubiquitination sites by exploiting amino acid pattern and properties.
 Chen Z, Zhou Y, Song J, Zhang Z.
 Biochim Biophys Acta. 2013 Aug;1834(8):1461-7. [PMID: 23603789]
 [5] Proteome-wide identification of ubiquitylation sites by conjugation of engineered lysine-less ubiquitin.
 Oshikawa K, Matsumoto M, Oyamada K, Nakayama KI.
 J Proteome Res. 2012 Feb 3;11(2):796-807. [PMID: 22053931] 

Functional Description

 Component of the ESCRT-II complex (endosomal sorting complex required for transport II), which is required for multivesicular body (MVB) formation and sorting of endosomal cargo proteins into MVBs. The MVB pathway mediates delivery of transmembrane proteins into the lumen of the lysosome for degradation. The ESCRT-II complex is probably involved in the recruitment of the ESCRT-III complex. The ESCRT-II complex may also play a role in transcription regulation, possibly via its interaction with ELL. The ESCRT-II complex may be involved in facilitating the budding of certain RNA viruses. 

Sequence Annotation

  

Keyword

 3D-structure; Complete proteome; Cytoplasm; Endosome; Membrane; Nucleus; Polymorphism; Protein transport; Reference proteome; Transcription; Transcription regulation; Transport. 

Sequence Source

 UniProt (SWISSPROT/TrEMBL); GenBank; EMBL 

Protein Length

 176 AA 

Protein Sequence

Gene Ontology

 GO:0005829; C:cytosol; TAS:Reactome.
 GO:0010008; C:endosome membrane; IEA:UniProtKB-SubCell.
 GO:0005739; C:mitochondrion; IEA:Compara.
 GO:0005654; C:nucleoplasm; IEA:UniProtKB-SubCell.
 GO:0016044; P:cellular membrane organization; TAS:Reactome.
 GO:0016197; P:endosomal transport; TAS:Reactome.
 GO:0015031; P:protein transport; IEA:UniProtKB-KW.
 GO:0006355; P:regulation of transcription, DNA-dependent; IEA:UniProtKB-KW.
 GO:0006351; P:transcription, DNA-dependent; IEA:UniProtKB-KW. 

Interpro

 IPR008570; ESCRT-II_cplx_vps25-sub.
 IPR014041; ESCRT-II_cplx_Vps25-sub_N.
 IPR011991; WHTH_DNA-bd_dom. 

Pfam

 PF05871; ESCRT-II 

SMART

  

PROSITE

  

PRINTS