CPLM-009345

Genbank Nucleotide ID

Heart- and neural crest derivatives-expressed protein 2

Protein Synonyms/Alias

Class A basic helix-loop-helix protein 26; bHLHa26; Deciduum, heart, autonomic nervous system and neural crest derivatives-expressed protein 2; dHAND

Homo sapiens (Human)

Position	Peptide	Type	References
183	VKEEKRKKELNEILK	ubiquitination	[1, 2, 3, 4]

[1] A proteome-wide, quantitative survey of in vivo ubiquitylation sites reveals widespread regulatory roles.
Wagner SA, Beli P, Weinert BT, Nielsen ML, Cox J, Mann M, Choudhary C.
Mol Cell Proteomics. 2011 Oct;10(10):M111.013284. [PMID: 21890473]
[2] Methods for quantification of in vivo changes in protein ubiquitination following proteasome and deubiquitinase inhibition.
Udeshi ND, Mani DR, Eisenhaure T, Mertins P, Jaffe JD, Clauser KR, Hacohen N, Carr SA.
Mol Cell Proteomics. 2012 May;11(5):148-59. [PMID: 22505724]
[3] Refined preparation and use of anti-diglycine remnant (K-ε-GG) antibody enables routine quantification of 10,000s of ubiquitination sites in single proteomics experiments.
Udeshi ND, Svinkina T, Mertins P, Kuhn E, Mani DR, Qiao JW, Carr SA.
Mol Cell Proteomics. 2013 Mar;12(3):825-31. [PMID: 23266961]
[4] hCKSAAP_UbSite: improved prediction of human ubiquitination sites by exploiting amino acid pattern and properties.
Chen Z, Zhou Y, Song J, Zhang Z.
Biochim Biophys Acta. 2013 Aug;1834(8):1461-7. [PMID: 23603789]

Functional Description

Essential for cardiac morphogenesis, particularly for the formation of the right ventricle and of the aortic arch arteries. Required for vascular development and regulation of angiogenesis, possibly through a VEGF signaling pathway. Plays also an important role in limb development, particularly in the establishment of anterior-posterior polarization, acting as an upstream regulator of sonic hedgehog (SHH) induction in the limb bud. Is involved in the development of branchial arches, which give rise to unique structures in the head and neck. Binds DNA on E-box consensus sequence 5'-CANNTG-3' (By similarity).

Angiogenesis; Complete proteome; Developmental protein; Differentiation; DNA-binding; Nucleus; Reference proteome; Transcription; Transcription regulation.

UniProt (SWISSPROT/TrEMBL); GenBank; EMBL

GO:0000790; C:nuclear chromatin; IDA:BHF-UCL.
GO:0005667; C:transcription factor complex; ISS:BHF-UCL.
GO:0033613; F:activating transcription factor binding; ISS:BHF-UCL.
GO:0003680; F:AT DNA binding; IDA:UniProtKB.
GO:0070888; F:E-box binding; IDA:UniProtKB.
GO:0042803; F:protein homodimerization activity; ISS:BHF-UCL.
GO:0001077; F:RNA polymerase II core promoter proximal region sequence-specific DNA binding transcription factor activity involved in positive regulation of transcription; IDA:UniProtKB.
GO:0000977; F:RNA polymerase II regulatory region sequence-specific DNA binding; IDA:BHF-UCL.
GO:0003713; F:transcription coactivator activity; ISS:BHF-UCL.
GO:0007512; P:adult heart development; IEP:BHF-UCL.
GO:0001525; P:angiogenesis; IEA:UniProtKB-KW.
GO:0006915; P:apoptotic process; ISS:BHF-UCL.
GO:0061309; P:cardiac neural crest cell development involved in outflow tract morphogenesis; ISS:BHF-UCL.
GO:0003253; P:cardiac neural crest cell migration involved in outflow tract morphogenesis; IEA:Compara.
GO:0003219; P:cardiac right ventricle formation; IEA:Compara.
GO:0060536; P:cartilage morphogenesis; IEA:Compara.
GO:0061325; P:cell proliferation involved in outflow tract morphogenesis; IEA:Compara.
GO:0060982; P:coronary artery morphogenesis; IEA:Compara.
GO:0042733; P:embryonic digit morphogenesis; IEA:Compara.
GO:0001947; P:heart looping; ISS:BHF-UCL.
GO:0001701; P:in utero embryonic development; ISS:BHF-UCL.
GO:0010463; P:mesenchymal cell proliferation; IEA:Compara.
GO:0010667; P:negative regulation of cardiac muscle cell apoptotic process; ISS:BHF-UCL.
GO:0043392; P:negative regulation of DNA binding; IEA:Compara.
GO:0045668; P:negative regulation of osteoblast differentiation; IEA:Compara.
GO:0043433; P:negative regulation of sequence-specific DNA binding transcription factor activity; IEA:Compara.
GO:0003357; P:noradrenergic neuron differentiation; NAS:BHF-UCL.
GO:0042475; P:odontogenesis of dentin-containing tooth; IEA:Compara.
GO:0060021; P:palate development; IEA:Compara.
GO:0048935; P:peripheral nervous system neuron development; IEA:Compara.
GO:2000764; P:positive regulation of semaphorin-plexin signaling pathway involved in outflow tract morphogenesis; ISS:BHF-UCL.
GO:2000763; P:positive regulation of transcription from RNA polymerase II promoter involved in norepinephrine biosynthetic process; ISS:BHF-UCL.
GO:2000679; P:positive regulation of transcription regulatory region DNA binding; IDA:BHF-UCL.
GO:0003266; P:regulation of secondary heart field cardioblast proliferation; ISS:BHF-UCL.
GO:0001967; P:suckling behavior; IEA:Compara.
GO:0048485; P:sympathetic nervous system development; IEA:Compara.
GO:0048538; P:thymus development; ISS:BHF-UCL.
GO:0043586; P:tongue development; IEA:Compara.
GO:0061032; P:visceral serous pericardium development; IEA:Compara.

IPR011598; bHLH_dom.