UniProt Accession

 TGS1_HUMAN; Q96RS0; A6NJQ5; Q5GH23; Q8TDG9; Q96QU3; Q9H5V3 

Genbank Protein ID

 AY028423; AF432215; AF286340; AC100817; AY534911; BC011999; AK026648 

Genbank Nucleotide ID

 AAK27730.1; AAL99922.1; AAK83025.1; AAT02709.1; AAH11999.1; BAB15516.1 

Protein Name

 Trimethylguanosine synthase 

Protein Synonyms/Alias

 CLL-associated antigen KW-2; Cap-specific guanine-N2 methyltransferase; Hepatocellular carcinoma-associated antigen 137; Nuclear receptor coactivator 6-interacting protein; PRIP-interacting protein with methyltransferase motif; PIMT; PIPMT 

Gene Name

 TGS1 

Gene Synonyms/Alias

 HCA137; NCOA6IP; PIMT 

Created Date

 July 27, 2013 

Organism

 Homo sapiens (Human) 

NCBI Taxa ID

 9606 

Lysine Modification

Position	Peptide	Type	References
5	***MCCEKWSRVAEM	ubiquitination	[1]
37	RAFVEDRKLYNLGLK	ubiquitination	[1]
44	KLYNLGLKGYYIRDS	ubiquitination	[1, 2]
110	FGRITAHKDFEVSMN	ubiquitination	[1]
175	TYELQSKKDTETENP	ubiquitination	[1]
456	FKYGSGQKYGGIPNF	ubiquitination	[1]
480	KNVKLKSKYLDMRRQ	ubiquitination	[1]
646	AAVPELAKYWAQRYR	ubiquitination	[1, 2]
663	SRFDDGIKLDREGWF	ubiquitination	[1, 3, 4]
676	WFSVTPEKIAEHIAG	ubiquitination	[1]
724	AIDIDPVKIALARNN	ubiquitination	[1]
836	NFLNNKLKTITAYFG	ubiquitination	[1]

Reference

 [1] Refined preparation and use of anti-diglycine remnant (K-ε-GG) antibody enables routine quantification of 10,000s of ubiquitination sites in single proteomics experiments.
 Udeshi ND, Svinkina T, Mertins P, Kuhn E, Mani DR, Qiao JW, Carr SA.
 Mol Cell Proteomics. 2013 Mar;12(3):825-31. [PMID: 23266961]
 [2] A proteome-wide, quantitative survey of in vivo ubiquitylation sites reveals widespread regulatory roles.
 Wagner SA, Beli P, Weinert BT, Nielsen ML, Cox J, Mann M, Choudhary C.
 Mol Cell Proteomics. 2011 Oct;10(10):M111.013284. [PMID: 21890473]
 [3] Systematic and quantitative assessment of the ubiquitin-modified proteome.
 Kim W, Bennett EJ, Huttlin EL, Guo A, Li J, Possemato A, Sowa ME, Rad R, Rush J, Comb MJ, Harper JW, Gygi SP.
 Mol Cell. 2011 Oct 21;44(2):325-40. [PMID: 21906983]
 [4] Integrated proteomic analysis of post-translational modifications by serial enrichment.
 Mertins P, Qiao JW, Patel J, Udeshi ND, Clauser KR, Mani DR, Burgess MW, Gillette MA, Jaffe JD, Carr SA.
 Nat Methods. 2013 Jul;10(7):634-7. [PMID: 23749302] 

Functional Description

 Catalyzes the 2 serial methylation steps for the conversion of the 7-monomethylguanosine (m(7)G) caps of snRNAs and snoRNAs to a 2,2,7-trimethylguanosine (m(2,2,7)G) cap structure. The enzyme is specific for guanine, and N7 methylation must precede N2 methylation. Hypermethylation of the m7G cap of U snRNAs leads to their concentration in nuclear foci, their colocalization with coilin and the formation of canonical Cajal bodies (CBs). Plays a role in transcriptional regulation. 

Sequence Annotation

 REGION 631 846 Sufficient for catalytic activity.
 BINDING 719 719 S-adenosyl-L-methionine (By similarity).
 BINDING 766 766 7-methylguanosine.
 MOD_RES 55 55 Phosphoserine.
 MOD_RES 60 60 Phosphothreonine.
 MOD_RES 85 85 Phosphoserine.
 MOD_RES 89 89 Phosphoserine.
 MOD_RES 154 154 Phosphoserine.
 MOD_RES 438 438 Phosphoserine.  

Keyword

 3D-structure; Complete proteome; Cytoplasm; Methyltransferase; Nucleus; Phosphoprotein; Polymorphism; Reference proteome; Transcription; Transcription regulation; Transferase. 

Sequence Source

 UniProt (SWISSPROT/TrEMBL); GenBank; EMBL 

Protein Length

 853 AA 

Protein Sequence

Gene Ontology

 GO:0015030; C:Cajal body; IEA:UniProtKB-SubCell.
 GO:0005829; C:cytosol; TAS:Reactome.
 GO:0005654; C:nucleoplasm; TAS:Reactome.
 GO:0030532; C:small nuclear ribonucleoprotein complex; IC:BHF-UCL.
 GO:0071164; F:RNA trimethylguanosine synthase activity; IDA:BHF-UCL.
 GO:0009452; P:7-methylguanosine RNA capping; IEA:InterPro.
 GO:0044255; P:cellular lipid metabolic process; TAS:Reactome.
 GO:0034660; P:ncRNA metabolic process; TAS:Reactome.
 GO:0006355; P:regulation of transcription, DNA-dependent; IEA:UniProtKB-KW.
 GO:0071167; P:ribonucleoprotein complex import into nucleus; IC:BHF-UCL.
 GO:0044281; P:small molecule metabolic process; TAS:Reactome.
 GO:0000387; P:spliceosomal snRNP assembly; TAS:Reactome.
 GO:0006351; P:transcription, DNA-dependent; IEA:UniProtKB-KW. 

Interpro

 IPR019012; RNA_cap_Gua-N2-MeTrfase. 

Pfam

 PF09445; Methyltransf_15 

SMART

  

PROSITE

  

PRINTS