CPLM 1.0 - Compendium of Protein Lysine Modification
TagContent
CPLM ID CPLM-012719
UniProt Accession
Genbank Protein ID
Genbank Nucleotide ID
Protein Name
 Alpha-mannosidase 2 
Protein Synonyms/Alias
 Golgi alpha-mannosidase II; AMan II; Man II; Mannosidase alpha class 2A member 1; Mannosyl-oligosaccharide 1,3-1,6-alpha-mannosidase 
Gene Name
 MAN2A1 
Gene Synonyms/Alias
 MANA2 
Created Date
 July 27, 2013 
Organism
 Homo sapiens (Human) 
NCBI Taxa ID
 9606 
Lysine Modification
Position
Peptide
Type
References
373ICCQFDFKRLPGGRFubiquitination[1]
887MKISSDIKSQNRFYTubiquitination[1]
Reference
 [1] Refined preparation and use of anti-diglycine remnant (K-ε-GG) antibody enables routine quantification of 10,000s of ubiquitination sites in single proteomics experiments.
 Udeshi ND, Svinkina T, Mertins P, Kuhn E, Mani DR, Qiao JW, Carr SA.
 Mol Cell Proteomics. 2013 Mar;12(3):825-31. [PMID: 23266961] 
Functional Description
 Catalyzes the first committed step in the biosynthesis of complex N-glycans. It controls conversion of high mannose to complex N-glycans; the final hydrolytic step in the N-glycan maturation pathway. 
Sequence Annotation
 ACT_SITE 289 289 Nucleophile (By similarity).
 METAL 175 175 Zinc (By similarity).
 METAL 177 177 Zinc (By similarity).
 METAL 289 289 Zinc (By similarity).
 METAL 569 569 Zinc (By similarity).
 MOD_RES 80 80 Phosphoserine (By similarity).
 MOD_RES 82 82 Phosphoserine (By similarity).
 CARBOHYD 78 78 N-linked (GlcNAc...).
 CARBOHYD 93 93 N-linked (GlcNAc...) (Potential).
 CARBOHYD 1125 1125 N-linked (GlcNAc...).  
Keyword
 Complete proteome; Disulfide bond; Glycoprotein; Glycosidase; Golgi apparatus; Hydrolase; Membrane; Metal-binding; Phosphoprotein; Reference proteome; Signal-anchor; Transmembrane; Transmembrane helix; Zinc. 
Sequence Source
 UniProt (SWISSPROT/TrEMBL); GenBank; EMBL 
Protein Length
 1144 AA 
Protein Sequence
MKLSRQFTVF GSAIFCVVIF SLYLMLDRGH LDYPRNPRRE GSFPQGQLSM LQEKIDHLER 60
LLAENNEIIS NIRDSVINLS ESVEDGPKSS QSNFSQGAGS HLLPSQLSLS VDTADCLFAS 120
QSGSHNSDVQ MLDVYSLISF DNPDGGVWKQ GFDITYESNE WDTEPLQVFV VPHSHNDPGW 180
LKTFNDYFRD KTQYIFNNMV LKLKEDSRRK FIWSEISYLS KWWDIIDIQK KDAVKSLIEN 240
GQLEIVTGGW VMPDEATPHY FALIDQLIEG HQWLENNIGV KPRSGWAIDP FGHSPTMAYL 300
LNRAGLSHML IQRVHYAVKK HFALHKTLEF FWRQNWDLGS VTDILCHMMP FYSYDIPHTC 360
GPDPKICCQF DFKRLPGGRF GCPWGVPPET IHPGNVQSRA RMLLDQYRKK SKLFRTKVLL 420
APLGDDFRYC EYTEWDLQFK NYQQLFDYMN SQSKFKVKIQ FGTLSDFFDA LDKADETQRD 480
KGQSMFPVLS GDFFTYADRD DHYWSGYFTS RPFYKRMDRI MESHLRAAEI LYYFALRQAH 540
KYKINKFLSS SLYTALTEAR RNLGLFQHHD AITGTAKDWV VVDYGTRLFH SLMVLEKIIG 600
NSAFLLILKD KLTYDSYSPD TFLEMDLKQK SQDSLPQKNI IRLSAEPRYL VVYNPLEQDR 660
ISLVSVYVSS PTVQVFSASG KPVEVQVSAV WDTANTISET AYEISFRAHI PPLGLKVYKI 720
LESASSNSHL ADYVLYKNKV EDSGIFTIKN MINTEEGITL ENSFVLLRFD QTGLMKQMMT 780
KEDGKHHEVN VQFSWYGTTI KRDKSGAYLF LPDGNAKPYV YTTPPFVRVT HGRIYSEVTC 840
FFDHVTHRVR LYHIQGIEGQ SVEVSNIVDI RKVYNREIAM KISSDIKSQN RFYTDLNGYQ 900
IQPRMTLSKL PLQANVYPMT TMAYIQDAKH RLTLLSAQSL GVSSLNSGQI EVIMDRRLMQ 960
DDNRGLEQGI QDNKITANLF RILLEKRSAV NTEEEKKSVS YPSLLSHITS SLMNHPVIPM 1020
ANKFSSPTLE LQGEFSPLQS SLPCDIHLVN LRTIQSKVGN GHSNEAALIL HRKGFDCRFS 1080
SKGTGLFCST TQGKILVQKL LNKFIVESLT PSSLSLMHSP PGTQNISEIN LSPMEISTFR 1140
IQLR 1144 
Gene Ontology
 GO:0000139; C:Golgi membrane; TAS:Reactome.
 GO:0016021; C:integral to membrane; IEA:UniProtKB-KW.
 GO:0030246; F:carbohydrate binding; IEA:InterPro.
 GO:0016799; F:hydrolase activity, hydrolyzing N-glycosyl compounds; IEA:Compara.
 GO:0004572; F:mannosyl-oligosaccharide 1,3-1,6-alpha-mannosidase activity; TAS:ProtInc.
 GO:0008270; F:zinc ion binding; IEA:InterPro.
 GO:0001701; P:in utero embryonic development; IEA:Compara.
 GO:0001889; P:liver development; IEA:Compara.
 GO:0048286; P:lung alveolus development; IEA:Compara.
 GO:0006013; P:mannose metabolic process; IEA:InterPro.
 GO:0007005; P:mitochondrion organization; IEA:Compara.
 GO:0006491; P:N-glycan processing; IEA:Compara.
 GO:0043687; P:post-translational protein modification; TAS:Reactome.
 GO:0018279; P:protein N-linked glycosylation via asparagine; TAS:Reactome.
 GO:0007585; P:respiratory gaseous exchange; IEA:Compara.
 GO:0007033; P:vacuole organization; IEA:Compara. 
Interpro
 IPR011013; Gal_mutarotase_SF_dom.
 IPR011330; Glyco_hydro/deAcase_b/a-brl.
 IPR013780; Glyco_hydro_13_b.
 IPR027291; Glyco_hydro_38/57_N.
 IPR011682; Glyco_hydro_38_C.
 IPR015341; Glyco_hydro_38_cen_dom.
 IPR000602; Glyco_hydro_38_N. 
Pfam
 PF09261; Alpha-mann_mid
 PF01074; Glyco_hydro_38
 PF07748; Glyco_hydro_38C 
SMART
 SM00872; Alpha-mann_mid 
PROSITE
  
PRINTS