CPLM 1.0 - Compendium of Protein Lysine Modification
TagContent
CPLM ID CPLM-021999
UniProt Accession
Genbank Protein ID
Genbank Nucleotide ID
Protein Name
 Hairy/enhancer-of-split related with YRPW motif-like protein 
Protein Synonyms/Alias
 hHeyL; Class B basic helix-loop-helix protein 33; bHLHb33; Hairy-related transcription factor 3; HRT-3; hHRT3 
Gene Name
 HEYL 
Gene Synonyms/Alias
 BHLHB33; HRT3 
Created Date
 July 27, 2013 
Organism
 Homo sapiens (Human) 
NCBI Taxa ID
 9606 
Lysine Modification
Position
Peptide
Type
References
76LVPTAFEKQGSSKLEubiquitination[1, 2]
84QGSSKLEKAEVLQMTubiquitination[2]
Reference
 [1] Systematic and quantitative assessment of the ubiquitin-modified proteome.
 Kim W, Bennett EJ, Huttlin EL, Guo A, Li J, Possemato A, Sowa ME, Rad R, Rush J, Comb MJ, Harper JW, Gygi SP.
 Mol Cell. 2011 Oct 21;44(2):325-40. [PMID: 21906983]
 [2] Refined preparation and use of anti-diglycine remnant (K-ε-GG) antibody enables routine quantification of 10,000s of ubiquitination sites in single proteomics experiments.
 Udeshi ND, Svinkina T, Mertins P, Kuhn E, Mani DR, Qiao JW, Carr SA.
 Mol Cell Proteomics. 2013 Mar;12(3):825-31. [PMID: 23266961] 
Functional Description
 Downstream effector of Notch signaling which may be required for cardiovascular development (By similarity). Transcriptional repressor which binds preferentially to the canonical E box sequence 5'-CACGTG-3' (By similarity). Represses transcription by the cardiac transcriptional activators GATA4 and GATA6. 
Sequence Annotation
 DOMAIN 43 98 bHLH.
 DOMAIN 116 153 Orange.
 REGION 42 111 Transcriptional repression and  
Keyword
 Complete proteome; Developmental protein; DNA-binding; Notch signaling pathway; Nucleus; Polymorphism; Reference proteome; Repressor; Transcription; Transcription regulation. 
Sequence Source
 UniProt (SWISSPROT/TrEMBL); GenBank; EMBL 
Protein Length
 328 AA 
Protein Sequence
MKRPKEPSGS DGESDGPIDV GQEGQLSQMA RPLSTPSSSQ MQARKKHRGI IEKRRRDRIN 60
SSLSELRRLV PTAFEKQGSS KLEKAEVLQM TVDHLKMLHA TGGTGFFDAR ALAVDFRSIG 120
FRECLTEVIR YLGVLEGPSS RADPVRIRLL SHLNSYAAEM EPSPTPTGPL AFPAWPWSFF 180
HSCPGLPALS NQLAILGRVP SPVLPGVSSP AYPIPALRTA PLRRATGIIL PARRNVLPSR 240
GASSTRRARP LERPATPVPV APSSRAARSS HIAPLLQSSS PTPPGPTGSA AYVAVPTPNS 300
SSPGPAGRPA GAMLYHSWVS EITEIGAF 328 
Gene Ontology
 GO:0005737; C:cytoplasm; ISS:UniProtKB.
 GO:0005654; C:nucleoplasm; TAS:Reactome.
 GO:0035939; F:microsatellite binding; IDA:UniProtKB.
 GO:0046982; F:protein heterodimerization activity; IDA:UniProtKB.
 GO:0001077; F:RNA polymerase II core promoter proximal region sequence-specific DNA binding transcription factor activity involved in positive regulation of transcription; IEA:Compara.
 GO:0000979; F:RNA polymerase II core promoter sequence-specific DNA binding; IEA:Compara.
 GO:0000983; F:RNA polymerase II core promoter sequence-specific DNA binding transcription factor activity; ISS:UniProtKB.
 GO:0001106; F:RNA polymerase II transcription corepressor activity; IEA:Compara.
 GO:0003714; F:transcription corepressor activity; IDA:UniProtKB.
 GO:0008134; F:transcription factor binding; NAS:UniProtKB.
 GO:0003181; P:atrioventricular valve morphogenesis; ISS:BHF-UCL.
 GO:0003208; P:cardiac ventricle morphogenesis; ISS:BHF-UCL.
 GO:0071773; P:cellular response to BMP stimulus; IEA:Compara.
 GO:0003198; P:epithelial to mesenchymal transition involved in endocardial cushion formation; ISS:UniProtKB.
 GO:0032835; P:glomerulus development; IEA:Compara.
 GO:2000824; P:negative regulation of androgen receptor activity; IDA:UniProtKB.
 GO:0060766; P:negative regulation of androgen receptor signaling pathway; IDA:UniProtKB.
 GO:0045892; P:negative regulation of transcription, DNA-dependent; IDA:UniProtKB.
 GO:0007219; P:Notch signaling pathway; IDA:UniProtKB.
 GO:0003151; P:outflow tract morphogenesis; ISS:BHF-UCL.
 GO:0045666; P:positive regulation of neuron differentiation; ISS:UniProtKB.
 GO:0045944; P:positive regulation of transcription from RNA polymerase II promoter; IDA:UniProtKB.
 GO:0072014; P:proximal tubule development; IEA:Compara.
 GO:0003184; P:pulmonary valve morphogenesis; ISS:BHF-UCL.
 GO:0060412; P:ventricular septum morphogenesis; ISS:BHF-UCL. 
Interpro
 IPR011598; bHLH_dom.
 IPR003650; Orange.
 IPR018352; Orange_subgr. 
Pfam
 PF07527; Hairy_orange
 PF00010; HLH 
SMART
 SM00353; HLH
 SM00511; ORANGE 
PROSITE
 PS50888; BHLH
 PS51054; ORANGE 
PRINTS