CPLM 1.0 - Compendium of Protein Lysine Modification
TagContent
CPLM ID CPLM-037408
UniProt Accession
Genbank Protein ID
Genbank Nucleotide ID
  
Protein Name
 Cullin-9 
Protein Synonyms/Alias
  
Gene Name
 CUL9 
Gene Synonyms/Alias
  
Created Date
 July 27, 2013 
Organism
 Homo sapiens (Human) 
NCBI Taxa ID
 9606 
Lysine Modification
Position
Peptide
Type
References
46LIRWSVLKCGEVGKVubiquitination[1]
87LGERALSKGLQHEPAubiquitination[1, 2, 3, 4, 5]
188QIRRSAGKMLQALAAubiquitination[1, 4]
571LNSQIYTKYGLLSNEubiquitination[3, 4]
627KTEAEPTKTRTETPMubiquitination[1]
986GAVRPLLKRLQQETQubiquitination[1]
1090ILSKVLDKHSAQLLLubiquitination[1]
1172KEDKCWEKVEVSSNPubiquitination[1]
1299GVEVLGPKPTFWPLFubiquitination[1]
1503DFVPRYCKLYEHLQRubiquitination[1, 5]
1657FQLQRLDKLFLEQEDubiquitination[3]
1718CYLYHPRKCLPTEFCubiquitination[1]
1853CLLVRILKAHGEKGLubiquitination[2, 3, 4]
1979QEVEGLMKQTVRQVQubiquitination[1]
2113SSPEVISKYEKALLRubiquitination[1, 2]
2116EVISKYEKALLRGYVubiquitination[1, 2]
2202AQSKHLAKLISKRCPubiquitination[1]
2217SCQAPIEKNEGCLHMubiquitination[1]
Reference
 [1] Refined preparation and use of anti-diglycine remnant (K-ε-GG) antibody enables routine quantification of 10,000s of ubiquitination sites in single proteomics experiments.
 Udeshi ND, Svinkina T, Mertins P, Kuhn E, Mani DR, Qiao JW, Carr SA.
 Mol Cell Proteomics. 2013 Mar;12(3):825-31. [PMID: 23266961]
 [2] A proteome-wide, quantitative survey of in vivo ubiquitylation sites reveals widespread regulatory roles.
 Wagner SA, Beli P, Weinert BT, Nielsen ML, Cox J, Mann M, Choudhary C.
 Mol Cell Proteomics. 2011 Oct;10(10):M111.013284. [PMID: 21890473]
 [3] Systematic and quantitative assessment of the ubiquitin-modified proteome.
 Kim W, Bennett EJ, Huttlin EL, Guo A, Li J, Possemato A, Sowa ME, Rad R, Rush J, Comb MJ, Harper JW, Gygi SP.
 Mol Cell. 2011 Oct 21;44(2):325-40. [PMID: 21906983]
 [4] Landscape of the PARKIN-dependent ubiquitylome in response to mitochondrial depolarization.
 Sarraf SA, Raman M, Guarani-Pereira V, Sowa ME, Huttlin EL, Gygi SP, Harper JW.
 Nature. 2013 Apr 18;496(7445):372-6. [PMID: 23503661]
 [5] Integrated proteomic analysis of post-translational modifications by serial enrichment.
 Mertins P, Qiao JW, Patel J, Udeshi ND, Clauser KR, Mani DR, Burgess MW, Gillette MA, Jaffe JD, Carr SA.
 Nat Methods. 2013 Jul;10(7):634-7. [PMID: 23749302
Functional Description
  
Sequence Annotation
  
Keyword
 Complete proteome; Metal-binding; Reference proteome; Repeat; Zinc; Zinc-finger. 
Sequence Source
 UniProt (SWISSPROT/TrEMBL); GenBank; EMBL 
Protein Length
 2489 AA 
Protein Sequence
MVGERHAGDL MVPLGPRLQA YPEELIRQRP GHDGHPEYLI RWSVLKCGEV GKVGVEEGKA 60
EHILMWLSAP EVYANCPGLL GERALSKGLQ HEPAGVSGSF PRDPGGLDEV AMGEMEADVQ 120
ALVRRAARQL AESGTPSLTA AVLHTIHVLS AYASIGPLTG VFRETGALDL LMHMLCNPEP 180
QIRRSAGKML QALAAHDAGS RAHVLLSLSQ QDGIEQHMDF DSRYTLLELF AETTSSEEHC 240
MAFEGIHLPQ IPGKLLFSLV KRYLCVTSLL DQLNSSPELG AGDQSSPCAT REKSRGQREL 300
EFSMAVGNLI SELVRSMGWA RNLSEQGMSP PRPTRSIFQP YISGPSLLLP TIVTTPRRQG 360
WVFRQRSEFS SRSGYGEYVQ QTLQPGMRVR MLDDYEEISA GDEGEFRQSN NGIPPVQVFW 420
QSTGRTYWVH WHMLEILGPE EATEDKASAA VEKGAGATVL GTAFPSWDWN PMDGLYPLPY 480
LQPEPQKNER VGYLTQAEWW ELLFFIKKLD LCEQQPIFQN LWKNLDETLG EKALGEISVS 540
VEMAESLLQV LSSRFEGSTL NDLLNSQIYT KYGLLSNEPS SSSTSRNHSC TPDPEEESKS 600
EASFSEEETE SLKAKAEAPK TEAEPTKTRT ETPMAQSDSQ LFNQLLVTEG MTLPTEMKEA 660
ASEMARALRG PGPRSSLDQH VAAVVATVQI SSLDTNLQLS GLSALSQAVE EVTERDHPLV 720
RPDRSLREKL VKMLVELLTN QVGEKMVVVQ ALRLLYLLMT KHEWRPLFAR EGGIYAVLVC 780
MQEYKTSVLV QQAGLAALKM LAVASSSEIP TFVTGRDSIH SLFDAQMTRE IFASIDSATR 840
PGSESLLLTV PAAVILMLNT EGCSSAARNG LLLLNLLLCN HHTLGDQIIT QELRDTLFRH 900
SGIAPRTEPM PTTRTILMML LNRYSEPPGS PERAALETPI IQGQDGSPEL LIRSLVGGPS 960
AELLLDLERV LCREGSPGGA VRPLLKRLQQ ETQPFLLLLR TLDAPGPNKT LLLSVLRVIT 1020
RLLDFPEAMV LPWHEVLEPC LNCLSGPSSD SEIVQELTCF LHRLASMHKD YAVVLCCLGA 1080
KEILSKVLDK HSAQLLLGCE LRDLVTECEK YAQLYSNLTS SILAGCIQMV LGQIEDHRRT 1140
HQPINIPFFD VFLRHLCQGS SVEVKEDKCW EKVEVSSNPH RASKLTDHNP KTYWESNGST 1200
GSHYITLHMH RGVLVRQLTL LVASEDSSYM PARVVVFGGD STSCIGTELN TVNVMPSASR 1260
VILLENLNRF WPIIQIRIKR CQQGGIDTRV RGVEVLGPKP TFWPLFREQL CRRTCLFYTI 1320
RAQAWSRDIA EDHRRLLQLC PRLNRVLRHE QNFADRFLPD DEAAQALGKT CWEALVSPLV 1380
QNITSPDAEG VSALGWLLDQ YLEQRETSRN PLSRAASFAS RVRRLCHLLV HVEPPPGPSP 1440
EPSTRPFSKN SKGRDRSPAP SPVLPSSSLR NITQCWLSVV QEQVSRFLAA AWRAPDFVPR 1500
YCKLYEHLQR AGSELFGPRA AFMLALRSGF SGALLQQSFL TAAHMSEQFA RYIDQQIQGG 1560
LIGGAPGVEM LGQLQRHLEP IMVLSGLELA TTFEHFYQHY MADRLLSFGS SWLEGAVLEQ 1620
IGLCFPNRLP QLMLQSLSTS EELQRQFHLF QLQRLDKLFL EQEDEEEKRL EEEEEEEEEE 1680
EAEKELFIED PSPAISILVL SPRCWPVSPL CYLYHPRKCL PTEFCDALDR FSSFYSQSQN 1740
HPVLDMGPHR RLQWTWLGRA ELQFGKQILH VSTVQMWLLL KFNQTEEVSV ETLLKDSDLS 1800
PELLLQALVP LTSGNGPLTL HEGQDFPHGE KDEGRTLEQK RNLLSCLLVR ILKAHGEKGL 1860
HIDQLVCLVL EAWQKGPNPP GTLGHTVAGG VACTSTDVLS CILHLLGQGY VKRRDDRPQI 1920
LMYAAPEPMG PCRGQADVPF CGSQSETSKP SPEAVATLAS LQLPAGRTMS PQEVEGLMKQ 1980
TVRQVQETLN LEPDVAQHLL AHSHWGAEQL LQSYSEDPEP LLLAAGLCVH QAQAVPVRPD 2040
HCPVCVSPLG CDDDLPSLCC MHYCCKSCWN EYLTTRIEQN LVLNCTCPIA DCPAQPTGAF 2100
IRAIVSSPEV ISKYEKALLR GYVESCSNLT WCTNPQGCDR ILCRQGLGCG TTCSKCGWAS 2160
CFNCSFPEAH YPASCGHMSQ WVDDGGYYDG MSVEAQSKHL AKLISKRCPS CQAPIEKNEG 2220
CLHMTCAKCN HGFCWRCLKS WKPNHKDYYN CSAMVSKAAR QEKRFQDYNE RCTFHHQARE 2280
FAVNLRNRVS AIHEVPPPRS FTFLNDACQG LEQARKVLAY ACVYSFYSQD AEYMDVVEQQ 2340
TENLELHTNA LQILLEETLL RCRDLASSLR LLRADCLSTG MELLRRIQER LLAILQHSAQ 2400
DFRVGLQSPS VEAWEAKGPN MPGSQPQASS GPEAEEEEED DEDDVPEWQQ DEFDEELDND 2460
SFSYDESENL DQETFFFGDE EEDEDEAYD 2489 
Gene Ontology
 GO:0031461; C:cullin-RING ubiquitin ligase complex; IEA:InterPro.
 GO:0008270; F:zinc ion binding; IEA:InterPro.
 GO:0006511; P:ubiquitin-dependent protein catabolic process; IEA:InterPro. 
Interpro
 IPR004939; APC_su10/DOC_dom.
 IPR011989; ARM-like.
 IPR016024; ARM-type_fold.
 IPR021097; CPH_domain.
 IPR016157; Cullin_CS.
 IPR016158; Cullin_homology.
 IPR001373; Cullin_N.
 IPR019559; Cullin_neddylation_domain.
 IPR008979; Galactose-bd-like.
 IPR014722; Rib_L2_dom2.
 IPR011991; WHTH_DNA-bd_dom.
 IPR002867; Znf_C6HC.
 IPR001841; Znf_RING.
 IPR017907; Znf_RING_CS. 
Pfam
 PF03256; APC10
 PF11515; Cul7
 PF00888; Cullin
 PF01485; IBR 
SMART
 SM00884; Cullin_Nedd8
 SM00647; IBR
 SM00184; RING 
PROSITE
 PS01256; CULLIN_1
 PS50069; CULLIN_2
 PS51284; DOC
 PS00518; ZF_RING_1
 PS50089; ZF_RING_2 
PRINTS