CPLM 1.0 - Compendium of Protein Lysine Modification
TagContent
CPLM ID CPLM-011140
UniProt Accession
Genbank Protein ID
Genbank Nucleotide ID
Protein Name
 CTD kinase subunit alpha 
Protein Synonyms/Alias
 CTDK-I subunit alpha; CTD kinase 58 kDa subunit; CTD kinase subunit 1 
Gene Name
 CTK1 
Gene Synonyms/Alias
 YKL139W 
Created Date
 July 27, 2013 
Organism
 Saccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast) 
NCBI Taxa ID
 559292 
Lysine Modification
Position
Peptide
Type
References
308KILHRDVKGSNILIDubiquitination[1]
Reference
 [1] Global analysis of phosphorylation and ubiquitylation cross-talk in protein degradation.
 Swaney DL, Beltrao P, Starita L, Guo A, Rush J, Fields S, Krogan NJ, Villén J.
 Nat Methods. 2013 Jul;10(7):676-82. [PMID: 23749301] 
Functional Description
 Catalytic subunit of the CTDK-I complex, which hyperphosphorylates the C-terminal heptapeptide repeat domain (CTD) of the largest RNA polymerase II subunit. CTDK-I phosphorylates 'Ser-5' if the CTD substrate is not phosphorylated at 'Ser-5', but will phosphorylate 'Ser-2' of a CTD substrate if 'Ser-5' is already phosphorylated. CTDK-I is also more reactive toward substrates that are prephosphorylated at 'Ser-2' or 'Ser-5' compared with an unphosphorylated CTD substrate, therefore efficiently creating doubly phosphorylated CTD repeats. Involved in RNA polymerase II transcriptional elongation, and through PTI1, pre-mRNA 3'-end processing. Participates in both positive and negative regulation of CTD phosphorylation. Required for DNA damage induced transcription, including the expression of the RNR genes, and reprogramming of gene expression upon amino acid starvation. Required for SET2 mediated H3K36 methylation. Also regulates H3K4 methylation. Controls the maintenance of suppressive chromatin in the coding regions of genes by both promoting H3K36 methylation, which leads to histone deacetylation, and catalyzing phosphorylation of the CTD required to localize H3K4 chromatin modification specifically to the 5' ends of genes, thereby creating a boundary for H3K4 methylation that prevents a mark associated with transcriptional initiation from spreading into the bodies of genes. Involved in RNA polymerase I transcription. Involved in telomere maintenance. Acts together with SNF1 to induce GSY2 transcription in response to glucose limitation. Involved in the adaptation to alternative carbon sources, including galactose, glycerol and ethanol, but not raffinose. Required for the integrity of the rDNA locus. Functions in translation elongation by enhancing decoding fidelity. Needed for translational accuracy by phosphorylating RPS2. 
Sequence Annotation
 DOMAIN 183 469 Protein kinase.
 NP_BIND 189 197 ATP (By similarity).
 MOTIF 37 44 Nuclear localization signal (Potential).
 ACT_SITE 306 306 Proton acceptor (By similarity).
 BINDING 212 212 ATP (By similarity).
 MOD_RES 14 14 Phosphoserine; by autocatalysis (By
 MOD_RES 338 338 Phosphothreonine.  
Keyword
 ATP-binding; Complete proteome; Cytoplasm; DNA damage; Kinase; mRNA processing; Nucleotide-binding; Nucleus; Phosphoprotein; Protein biosynthesis; Reference proteome; Serine/threonine-protein kinase; Stress response; Transcription; Transferase. 
Sequence Source
 UniProt (SWISSPROT/TrEMBL); GenBank; EMBL 
Protein Length
 528 AA 
Protein Sequence
MSYNNGNTYS KSYSRNNKRP LFGKRSPNPQ SLARPPPPKR IRTDSGYQSN MDNISSHRVN 60
SNDQPGHTKS RGNNNLSRYN DTSFQTSSRY QGSRYNNNNT SYENRPKSIK RDETKAEFLS 120
HLPKGPKSVE KSRYNNSSNT SNDIKNGYHA SKYYNHKGQE GRSVIAKKVP VSVLTQQRST 180
SVYLRIMQVG EGTYGKVYKA KNTNTEKLVA LKKLRLQGER EGFPITSIRE IKLLQSFDHP 240
NVSTIKEIMV ESQKTVYMIF EYADNDLSGL LLNKEVQISH SQCKHLFKQL LLGMEYLHDN 300
KILHRDVKGS NILIDNQGNL KITDFGLARK MNSRADYTNR VITLWYRPPE LLLGTTNYGT 360
EVDMWGCGCL LVELFNKTAI FQGSNELEQI ESIFKIMGTP TINSWPTLYD MPWFFMIMPQ 420
QTTKYVNNFS EKFKSVLPSS KCLQLAINLL CYDQTKRFSA TEALQSDYFK EEPKPEPLVL 480
DGLVSCHEYE VKLARKQKRP NILSTNTNNK GNGNSNNNNN NNNDDDDK 528 
Gene Ontology
 GO:0032806; C:carboxy-terminal domain protein kinase complex; IPI:SGD.
 GO:0005737; C:cytoplasm; IEA:UniProtKB-SubCell.
 GO:0005730; C:nucleolus; IDA:SGD.
 GO:0005654; C:nucleoplasm; IDA:SGD.
 GO:0005524; F:ATP binding; IEA:UniProtKB-KW.
 GO:0004693; F:cyclin-dependent protein serine/threonine kinase activity; IDA:SGD.
 GO:0008353; F:RNA polymerase II carboxy-terminal domain kinase activity; IEA:EC.
 GO:0031124; P:mRNA 3'-end processing; IMP:SGD.
 GO:0018105; P:peptidyl-serine phosphorylation; IDA:SGD.
 GO:0070816; P:phosphorylation of RNA polymerase II C-terminal domain; IMP:SGD.
 GO:0032786; P:positive regulation of DNA-dependent transcription, elongation; IDA:SGD.
 GO:0045943; P:positive regulation of transcription from RNA polymerase I promoter; IMP:SGD.
 GO:0045903; P:positive regulation of translational fidelity; IMP:SGD.
 GO:0006974; P:response to DNA damage stimulus; IEA:UniProtKB-KW.
 GO:0006351; P:transcription, DNA-dependent; IEA:UniProtKB-KW.
 GO:0006412; P:translation; IEA:UniProtKB-KW. 
Interpro
 IPR011009; Kinase-like_dom.
 IPR000719; Prot_kinase_cat_dom.
 IPR017441; Protein_kinase_ATP_BS.
 IPR002290; Ser/Thr_dual-sp_kinase_dom.
 IPR008271; Ser/Thr_kinase_AS. 
Pfam
 PF00069; Pkinase 
SMART
 SM00220; S_TKc 
PROSITE
 PS00107; PROTEIN_KINASE_ATP
 PS50011; PROTEIN_KINASE_DOM
 PS00108; PROTEIN_KINASE_ST 
PRINTS