CPLM 1.0 - Compendium of Protein Lysine Modification
TagContent
CPLM ID CPLM-017922
UniProt Accession
Genbank Protein ID
Genbank Nucleotide ID
Protein Name
 Tetratricopeptide repeat protein 8 
Protein Synonyms/Alias
 TPR repeat protein 8; Bardet-Biedl syndrome 8 protein 
Gene Name
 TTC8 
Gene Synonyms/Alias
 BBS8 
Created Date
 July 27, 2013 
Organism
 Homo sapiens (Human) 
NCBI Taxa ID
 9606 
Lysine Modification
Position
Peptide
Type
References
54HQAAWILKARALTEMubiquitination[1, 2]
279KQFKSALKQQEMVDTubiquitination[2]
308VTALNLFKQGLDKFPubiquitination[2]
342SSAAEYYKEVLKQDNubiquitination[2]
503NFATISDKIGDLQRSubiquitination[2]
516RSYVAAQKSEAAFPDubiquitination[2]
532VDTQHLIKQLRQHFAubiquitination[1, 2]
Reference
 [1] A proteome-wide, quantitative survey of in vivo ubiquitylation sites reveals widespread regulatory roles.
 Wagner SA, Beli P, Weinert BT, Nielsen ML, Cox J, Mann M, Choudhary C.
 Mol Cell Proteomics. 2011 Oct;10(10):M111.013284. [PMID: 21890473]
 [2] Systematic and quantitative assessment of the ubiquitin-modified proteome.
 Kim W, Bennett EJ, Huttlin EL, Guo A, Li J, Possemato A, Sowa ME, Rad R, Rush J, Comb MJ, Harper JW, Gygi SP.
 Mol Cell. 2011 Oct 21;44(2):325-40. [PMID: 21906983] 
Functional Description
 The BBSome complex is required for ciliogenesis but is dispensable for centriolar satellite function. This ciliogenic function is mediated in part by the Rab8 GDP/GTP exchange factor, which localizes to the basal body and contacts the BBSome. Rab8(GTP) enters the primary cilium and promotes extension of the ciliary membrane. Firstly the BBSome associates with the ciliary membrane and binds to RAB3IP/Rabin8, the guanosyl exchange factor (GEF) for Rab8 and then the Rab8-GTP localizes to the cilium and promotes docking and fusion of carrier vesicles to the base of the ciliary membrane. 
Sequence Annotation
 REPEAT 14 47 TPR 1.
 REPEAT 251 284 TPR 2.
 REPEAT 285 317 TPR 3.
 REPEAT 318 351 TPR 4.
 REPEAT 352 385 TPR 5.
 REPEAT 386 419 TPR 6.
 REPEAT 423 456 TPR 7.
 REPEAT 457 490 TPR 8.  
Keyword
 Alternative splicing; Bardet-Biedl syndrome; Cell membrane; Cell projection; Ciliopathy; Cilium; Cilium biogenesis/degradation; Complete proteome; Cytoplasm; Cytoskeleton; Disease mutation; Membrane; Mental retardation; Obesity; Reference proteome; Repeat; Retinitis pigmentosa; TPR repeat. 
Sequence Source
 UniProt (SWISSPROT/TrEMBL); GenBank; EMBL 
Protein Length
 541 AA 
Protein Sequence
MSSEMEPLLL AWSYFRRRKF QLCADLCTQM LEKSPYDQEP DPELPVHQAA WILKARALTE 60
MVYIDEIDVD QEGIAEMMLD ENAIAQVPRP GTSLKLPGTN QTGGPSQAVR PITQAGRPIT 120
GFLRPSTQSG RPGTMEQAIR TPRTAYTARP ITSSSGRFVR LGTASMLTSP DGPFINLSRL 180
NLTKYSQKPK LAKALFEYIF HHENDVKTIH LEDVVLHLGI YPFLLRNKNH IEKNALDLAA 240
LSTEHSQYKD WWWKVQIGKC YYRLGMYREA EKQFKSALKQ QEMVDTFLYL AKVYVSLDQP 300
VTALNLFKQG LDKFPGEVTL LCGIARIYEE MNNMSSAAEY YKEVLKQDNT HVEAIACIGS 360
NHFYSDQPEI ALRFYRRLLQ MGIYNGQLFN NLGLCCFYAQ QYDMTLTSFE RALSLAENEE 420
EAADVWYNLG HVAVGIGDTN LAHQCFRLAL VNNNNHAEAY NNLAVLEMRK GHVEQARALL 480
QTASSLAPHM YEPHFNFATI SDKIGDLQRS YVAAQKSEAA FPDHVDTQHL IKQLRQHFAM 540
L 541 
Gene Ontology
 GO:0034464; C:BBSome; IDA:BHF-UCL.
 GO:0005813; C:centrosome; IDA:BHF-UCL.
 GO:0005929; C:cilium; IDA:BHF-UCL.
 GO:0060170; C:cilium membrane; IEA:UniProtKB-SubCell.
 GO:0005737; C:cytoplasm; IEA:UniProtKB-SubCell.
 GO:0005932; C:microtubule basal body; IDA:BHF-UCL.
 GO:0032391; C:photoreceptor connecting cilium; IEA:Compara.
 GO:0042384; P:cilium assembly; TAS:BHF-UCL.
 GO:0048560; P:establishment of anatomical structure orientation; IMP:BHF-UCL.
 GO:0045444; P:fat cell differentiation; IEA:Compara.
 GO:0050893; P:sensory processing; TAS:BHF-UCL. 
Interpro
 IPR001440; TPR-1.
 IPR013026; TPR-contain_dom.
 IPR011990; TPR-like_helical.
 IPR013105; TPR_2.
 IPR019734; TPR_repeat. 
Pfam
 PF00515; TPR_1
 PF07719; TPR_2
 PF13181; TPR_8 
SMART
 SM00028; TPR 
PROSITE
 PS50005; TPR
 PS50293; TPR_REGION 
PRINTS