CPLM 1.0 - Compendium of Protein Lysine Modification
TagContent
CPLM ID CPLM-022847
UniProt Accession
Genbank Protein ID
Genbank Nucleotide ID
Protein Name
 tRNA (guanine-N(7)-)-methyltransferase 
Protein Synonyms/Alias
 Methyltransferase-like protein 1; tRNA (guanine(46)-N(7))-methyltransferase; tRNA(m7G46)-methyltransferase 
Gene Name
 METTL1 
Gene Synonyms/Alias
 C12orf1 
Created Date
 July 27, 2013 
Organism
 Homo sapiens (Human) 
NCBI Taxa ID
 9606 
Lysine Modification
Position
Peptide
Type
References
18AEAPPPQKRYYRQRAubiquitination[1]
111LGLEIRVKVSDYVQDubiquitination[2]
242GTSTEEGKKVLRNGGubiquitination[1]
243TSTEEGKKVLRNGGKubiquitination[2]
250KVLRNGGKNFPAIFRubiquitination[2, 3, 4, 5]
Reference
 [1] Systematic and quantitative assessment of the ubiquitin-modified proteome.
 Kim W, Bennett EJ, Huttlin EL, Guo A, Li J, Possemato A, Sowa ME, Rad R, Rush J, Comb MJ, Harper JW, Gygi SP.
 Mol Cell. 2011 Oct 21;44(2):325-40. [PMID: 21906983]
 [2] Refined preparation and use of anti-diglycine remnant (K-ε-GG) antibody enables routine quantification of 10,000s of ubiquitination sites in single proteomics experiments.
 Udeshi ND, Svinkina T, Mertins P, Kuhn E, Mani DR, Qiao JW, Carr SA.
 Mol Cell Proteomics. 2013 Mar;12(3):825-31. [PMID: 23266961]
 [3] A proteome-wide, quantitative survey of in vivo ubiquitylation sites reveals widespread regulatory roles.
 Wagner SA, Beli P, Weinert BT, Nielsen ML, Cox J, Mann M, Choudhary C.
 Mol Cell Proteomics. 2011 Oct;10(10):M111.013284. [PMID: 21890473]
 [4] Systems-wide analysis of ubiquitylation dynamics reveals a key role for PAF15 ubiquitylation in DNA-damage bypass.
 Povlsen LK, Beli P, Wagner SA, Poulsen SL, Sylvestersen KB, Poulsen JW, Nielsen ML, Bekker-Jensen S, Mailand N, Choudhary C.
 Nat Cell Biol. 2012 Oct;14(10):1089-98. [PMID: 23000965]
 [5] hCKSAAP_UbSite: improved prediction of human ubiquitination sites by exploiting amino acid pattern and properties.
 Chen Z, Zhou Y, Song J, Zhang Z.
 Biochim Biophys Acta. 2013 Aug;1834(8):1461-7. [PMID: 23603789] 
Functional Description
 Catalyzes the formation of N(7)-methylguanine at position 46 (m7G46) in tRNA. 
Sequence Annotation
 REGION 107 108 S-adenosyl-L-methionine binding.
 REGION 140 141 S-adenosyl-L-methionine binding.
 REGION 238 240 S-adenosyl-L-methionine binding.
 ACT_SITE 163 163 By similarity.
 BINDING 84 84 S-adenosyl-L-methionine; via carbonyl
 BINDING 160 160 S-adenosyl-L-methionine; via carbonyl
 MOD_RES 2 2 N-acetylalanine.
 MOD_RES 27 27 Phosphoserine; by PKB and RPS6KA3.  
Keyword
 3D-structure; Acetylation; Alternative splicing; Complete proteome; Methyltransferase; Nucleus; Phosphoprotein; Reference proteome; RNA-binding; S-adenosyl-L-methionine; Transferase; tRNA processing; tRNA-binding. 
Sequence Source
 UniProt (SWISSPROT/TrEMBL); GenBank; EMBL 
Protein Length
 276 AA 
Protein Sequence
MAAETRNVAG AEAPPPQKRY YRQRAHSNPM ADHTLRYPVK PEEMDWSELY PEFFAPLTQN 60
QSHDDPKDKK EKRAQAQVEF ADIGCGYGGL LVELSPLFPD TLILGLEIRV KVSDYVQDRI 120
RALRAAPAGG FQNIACLRSN AMKHLPNFFY KGQLTKMFFL FPDPHFKRTK HKWRIISPTL 180
LAEYAYVLRV GGLVYTITDV LELHDWMCTH FEEHPLFERV PLEDLSEDPV VGHLGTSTEE 240
GKKVLRNGGK NFPAIFRRIQ DPVLQAVTSQ TSLPGH 276 
Gene Ontology
 GO:0005737; C:cytoplasm; IDA:HPA.
 GO:0005634; C:nucleus; IDA:UniProtKB.
 GO:0008176; F:tRNA (guanine-N7-)-methyltransferase activity; IDA:UniProtKB.
 GO:0000049; F:tRNA binding; IEA:UniProtKB-KW. 
Interpro
 IPR003358; tRNA_(Gua-N-7)_MeTrfase.
 IPR025763; tRNA_(Gua-N-7)_MeTrfase_euk. 
Pfam
 PF02390; Methyltransf_4 
SMART
  
PROSITE
  
PRINTS