CPLM 1.0 - Compendium of Protein Lysine Modification
TagContent
CPLM ID CPLM-002625
UniProt Accession
Genbank Protein ID
Genbank Nucleotide ID
Protein Name
 6-phosphofructokinase, muscle type 
Protein Synonyms/Alias
 Phosphofructo-1-kinase isozyme A; PFK-A; Phosphofructokinase-M; Phosphofructokinase 1; Phosphohexokinase 
Gene Name
 PFKM 
Gene Synonyms/Alias
 PFKX 
Created Date
 July 27, 2013 
Organism
 Homo sapiens (Human) 
NCBI Taxa ID
 9606 
Lysine Modification
Position
Peptide
Type
References
16AKTLGIGKAIAVLTSubiquitination[1]
107RAAYNLVKRGITNLCubiquitination[1]
141DLLSDLQKAGKITDEubiquitination[1]
144SDLQKAGKITDEEATubiquitination[1]
272GAIDKNGKPITSEDIubiquitination[1, 2]
280PITSEDIKNGSRMGVubiquitination[1, 2]
329QVTKDVTKAMDEKKFubiquitination[1]
335TKAMDEKKFDEALKLubiquitination[1]
341KKFDEALKLRGRSFMubiquitination[1]
435WTGQGGSKLGTKRTLubiquitination[1]
584NVEHLVQKMKTTVKRubiquitination[1]
625KGIFDSRKNVLGHMQubiquitination[1]
647FDRNFATKMGAKAMNubiquitination[1]
651FATKMGAKAMNWMSGubiquitination[1]
696FQPVAELKDQTDFEHubiquitination[1]
Reference
 [1] Landscape of the PARKIN-dependent ubiquitylome in response to mitochondrial depolarization.
 Sarraf SA, Raman M, Guarani-Pereira V, Sowa ME, Huttlin EL, Gygi SP, Harper JW.
 Nature. 2013 Apr 18;496(7445):372-6. [PMID: 23503661]
 [2] Systematic and quantitative assessment of the ubiquitin-modified proteome.
 Kim W, Bennett EJ, Huttlin EL, Guo A, Li J, Possemato A, Sowa ME, Rad R, Rush J, Comb MJ, Harper JW, Gygi SP.
 Mol Cell. 2011 Oct 21;44(2):325-40. [PMID: 21906983] 
Functional Description
 Catalyzes the third step of glycolysis, the phosphorylation of fructose-6-phosphate (F6P) by ATP to generate fructose-1,6-bisphosphate (FBP) and ADP. 
Sequence Annotation
 NP_BIND 35 39 ATP (By similarity).
 NP_BIND 193 197 ATP (By similarity).
 NP_BIND 210 226 ATP (By similarity).
 ACT_SITE 166 166 Proton acceptor (By similarity).
 METAL 224 224 Magnesium; via carbonyl oxygen (By
 BINDING 201 201 Substrate (By similarity).
 BINDING 292 292 Substrate (By similarity).
 BINDING 298 298 Substrate (By similarity).
 BINDING 301 301 Substrate (By similarity).
 MOD_RES 2 2 N-acetylthreonine (By similarity).
 MOD_RES 667 667 Phosphoserine.
 MOD_RES 775 775 Phosphoserine (By similarity).
 CARBOHYD 530 530 O-linked (GlcNAc...) (By similarity).  
Keyword
 Acetylation; Allosteric enzyme; Alternative splicing; ATP-binding; Complete proteome; Disease mutation; Glycogen storage disease; Glycolysis; Glycoprotein; Kinase; Magnesium; Metal-binding; Nucleotide-binding; Phosphoprotein; Reference proteome; Repeat; Transferase. 
Sequence Source
 UniProt (SWISSPROT/TrEMBL); GenBank; EMBL 
Protein Length
 780 AA 
Protein Sequence
MTHEEHHAAK TLGIGKAIAV LTSGGDAQGM NAAVRAVVRV GIFTGARVFF VHEGYQGLVD 60
GGDHIKEATW ESVSMMLQLG GTVIGSARCK DFREREGRLR AAYNLVKRGI TNLCVIGGDG 120
SLTGADTFRS EWSDLLSDLQ KAGKITDEEA TKSSYLNIVG LVGSIDNDFC GTDMTIGTDS 180
ALHRIMEIVD AITTTAQSHQ RTFVLEVMGR HCGYLALVTS LSCGADWVFI PECPPDDDWE 240
EHLCRRLSET RTRGSRLNII IVAEGAIDKN GKPITSEDIK NGSRMGVEAV MALLEGTPDT 300
PACVVSLSGN QAVRLPLMEC VQVTKDVTKA MDEKKFDEAL KLRGRSFMNN WEVYKLLAHV 360
RPPVSKSGSH TVAVMNVGAP AAGMNAAVRS TVRIGLIQGN RVLVVHDGFE GLAKGQIEEA 420
GWSYVGGWTG QGGSKLGTKR TLPKKSFEQI SANITKFNIQ GLVIIGGFEA YTGGLELMEG 480
RKQFDELCIP FVVIPATVSN NVPGSDFSVG ADTALNTICT TCDRIKQSAA GTKRRVFIIE 540
TMGGYCGYLA TMAGLAAGAD AAYIFEEPFT IRDLQANVEH LVQKMKTTVK RGLVLRNEKC 600
NENYTTDFIF NLYSEEGKGI FDSRKNVLGH MQQGGSPTPF DRNFATKMGA KAMNWMSGKI 660
KESYRNGRIF ANTPDSGCVL GMRKRALVFQ PVAELKDQTD FEHRIPKEQW WLKLRPILKI 720
LAKYEIDLDT SDHAHLEHIT RKRSGEAAV 749 
Gene Ontology
 GO:0005945; C:6-phosphofructokinase complex; IDA:UniProtKB.
 GO:0016324; C:apical plasma membrane; IDA:UniProtKB.
 GO:0003872; F:6-phosphofructokinase activity; IDA:UniProtKB.
 GO:0005524; F:ATP binding; IDA:BHF-UCL.
 GO:0070061; F:fructose binding; IDA:BHF-UCL.
 GO:0046872; F:metal ion binding; IEA:UniProtKB-KW.
 GO:0006002; P:fructose 6-phosphate metabolic process; IDA:BHF-UCL.
 GO:0006096; P:glycolysis; IMP:UniProtKB.
 GO:0046716; P:muscle cell homeostasis; IMP:BHF-UCL.
 GO:0051259; P:protein oligomerization; IDA:BHF-UCL.
 GO:0044281; P:small molecule metabolic process; TAS:Reactome. 
Interpro
 IPR009161; 6-phosphofructokinase_euk.
 IPR022953; Phosphofructokinase.
 IPR015912; Phosphofructokinase_CS.
 IPR000023; Phosphofructokinase_dom. 
Pfam
 PF00365; PFK 
SMART
  
PROSITE
 PS00433; PHOSPHOFRUCTOKINASE 
PRINTS
 PR00476; PHFRCTKINASE.