UniProt Accession

 CIR1A_HUMAN; Q969X6; Q8NCD9; Q8TF14; Q96SP0; Q96SR9; Q96SZ9; Q96T13; Q9BWK6 

Genbank Protein ID

 AB075868; AK027419; AK027445; AK027584; AK027634; AK027675; AK074795; BC000167; BC009348 

Genbank Nucleotide ID

 BAB85574.2; BAB55100.1; BAB55116.1; BAB55212.1; BAB55251.1; BAB55287.1; BAC11214.1; AAH00167.1; AAH09348.1 

Protein Name

 Cirhin 

Protein Synonyms/Alias

  

Gene Name

 CIRH1A 

Gene Synonyms/Alias

 KIAA1988 

Created Date

 July 27, 2013 

Organism

 Homo sapiens (Human) 

NCBI Taxa ID

 9606 

Lysine Modification

Position	Peptide	Type	References
5	***MGEFKVHRVRFF	ubiquitination	[1]
53	SANYFQEKFFPGHES	ubiquitination	[1, 2]
134	LFQITPDKIQFERNF	ubiquitination	[1, 3, 4]
181	KSGSAVHKMIVDRQY	ubiquitination	[1, 2]
193	RQYMGVSKRKCIVWG	ubiquitination	[2]
281	EKQWVRTKPFQHHTH	ubiquitination	[1]
317	VFRPLMEKVEVKNYD	ubiquitination	[1]
321	LMEKVEVKNYDAALR	ubiquitination	[1, 2, 3, 4]
329	NYDAALRKITFPHRC	ubiquitination	[1]
368	GSTVATGKNGDTLPL	ubiquitination	[1, 2]
377	GDTLPLSKNADHLLH	ubiquitination	[1]
386	ADHLLHLKTKGPENI	ubiquitination	[1]
428	EHDNISLKRVSKMPA	ubiquitination	[1, 2]
569	DWSRTVQKQGFHHLW	ubiquitination	[1, 2, 3, 4, 5]
617	SLPLPNDKTLLYNPF	ubiquitination	[1, 2, 3, 4]
648	FKISKIYKPLLFMDL	ubiquitination	[2]
680	AQLPPPIKKKKFGT*	ubiquitination	[2]
681	QLPPPIKKKKFGT**	ubiquitination	[1]

Reference

 [1] Refined preparation and use of anti-diglycine remnant (K-ε-GG) antibody enables routine quantification of 10,000s of ubiquitination sites in single proteomics experiments.
 Udeshi ND, Svinkina T, Mertins P, Kuhn E, Mani DR, Qiao JW, Carr SA.
 Mol Cell Proteomics. 2013 Mar;12(3):825-31. [PMID: 23266961]
 [2] Systematic and quantitative assessment of the ubiquitin-modified proteome.
 Kim W, Bennett EJ, Huttlin EL, Guo A, Li J, Possemato A, Sowa ME, Rad R, Rush J, Comb MJ, Harper JW, Gygi SP.
 Mol Cell. 2011 Oct 21;44(2):325-40. [PMID: 21906983]
 [3] A proteome-wide, quantitative survey of in vivo ubiquitylation sites reveals widespread regulatory roles.
 Wagner SA, Beli P, Weinert BT, Nielsen ML, Cox J, Mann M, Choudhary C.
 Mol Cell Proteomics. 2011 Oct;10(10):M111.013284. [PMID: 21890473]
 [4] hCKSAAP_UbSite: improved prediction of human ubiquitination sites by exploiting amino acid pattern and properties.
 Chen Z, Zhou Y, Song J, Zhang Z.
 Biochim Biophys Acta. 2013 Aug;1834(8):1461-7. [PMID: 23603789]
 [5] Systems-wide analysis of ubiquitylation dynamics reveals a key role for PAF15 ubiquitylation in DNA-damage bypass.
 Povlsen LK, Beli P, Wagner SA, Poulsen SL, Sylvestersen KB, Poulsen JW, Nielsen ML, Bekker-Jensen S, Mailand N, Choudhary C.
 Nat Cell Biol. 2012 Oct;14(10):1089-98. [PMID: 23000965] 

Functional Description

 May be a transcriptional regulator. Acts as a positive regulator of HIVEP1 which specifically binds to the DNA sequence 5'-GGGACTTTCC-3' found in enhancer elements of numerous viral promoters such as those of HIV-1, SV40, or CMV. 

Sequence Annotation

 REPEAT 14 53 WD 1.
 REPEAT 57 96 WD 2.
 REPEAT 99 138 WD 3.
 REPEAT 143 181 WD 4.
 REPEAT 193 231 WD 5.
 REPEAT 234 273 WD 6.
 REPEAT 285 322 WD 7.
 REPEAT 324 359 WD 8.
 REPEAT 435 475 WD 9.
 REPEAT 482 521 WD 10.
 REPEAT 524 563 WD 11.  

Keyword

 Alternative splicing; Complete proteome; Disease mutation; Nucleus; Polymorphism; Reference proteome; Repeat; Transcription; Transcription regulation; WD repeat. 

Sequence Source

 UniProt (SWISSPROT/TrEMBL); GenBank; EMBL 

Protein Length

 686 AA 

Protein Sequence

Gene Ontology

 GO:0005730; C:nucleolus; IDA:UniProtKB.
 GO:0006355; P:regulation of transcription, DNA-dependent; IDA:UniProtKB.
 GO:0006351; P:transcription, DNA-dependent; IEA:UniProtKB-KW. 

Interpro

 IPR011047; Quinonprotein_ADH-like_supfam.
 IPR015943; WD40/YVTN_repeat-like_dom.
 IPR001680; WD40_repeat.
 IPR017986; WD40_repeat_dom. 

Pfam

 PF00400; WD40 

SMART

 SM00320; WD40 

PROSITE

 PS00678; WD_REPEATS_1
 PS50082; WD_REPEATS_2
 PS50294; WD_REPEATS_REGION 

PRINTS