CPLM 1.0 - Compendium of Protein Lysine Modification
TagContent
CPLM ID CPLM-032830
UniProt Accession
Genbank Protein ID
Genbank Nucleotide ID
Protein Name
 ATG12 autophagy related 12 homolog (S. cerevisiae), isoform CRA_b 
Protein Synonyms/Alias
 Atg12; Ubiquitin-like protein ATG12 
Gene Name
 ATG12 
Gene Synonyms/Alias
 hCG_36968 
Created Date
 July 27, 2013 
Organism
 Homo sapiens (Human) 
NCBI Taxa ID
 9606 
Lysine Modification
Position
Peptide
Type
References
107KKIDILLKAVGDTPIubiquitination[1, 2]
116VGDTPIMKTKKWAVEubiquitination[1, 2, 3, 4]
118DTPIMKTKKWAVERTubiquitination[2]
119TPIMKTKKWAVERTRubiquitination[2]
136QGLIDFIKKFLKLVAubiquitination[1, 2, 3, 5, 6]
Reference
 [1] Systematic and quantitative assessment of the ubiquitin-modified proteome.
 Kim W, Bennett EJ, Huttlin EL, Guo A, Li J, Possemato A, Sowa ME, Rad R, Rush J, Comb MJ, Harper JW, Gygi SP.
 Mol Cell. 2011 Oct 21;44(2):325-40. [PMID: 21906983]
 [2] Refined preparation and use of anti-diglycine remnant (K-ε-GG) antibody enables routine quantification of 10,000s of ubiquitination sites in single proteomics experiments.
 Udeshi ND, Svinkina T, Mertins P, Kuhn E, Mani DR, Qiao JW, Carr SA.
 Mol Cell Proteomics. 2013 Mar;12(3):825-31. [PMID: 23266961]
 [3] Landscape of the PARKIN-dependent ubiquitylome in response to mitochondrial depolarization.
 Sarraf SA, Raman M, Guarani-Pereira V, Sowa ME, Huttlin EL, Gygi SP, Harper JW.
 Nature. 2013 Apr 18;496(7445):372-6. [PMID: 23503661]
 [4] Integrated proteomic analysis of post-translational modifications by serial enrichment.
 Mertins P, Qiao JW, Patel J, Udeshi ND, Clauser KR, Mani DR, Burgess MW, Gillette MA, Jaffe JD, Carr SA.
 Nat Methods. 2013 Jul;10(7):634-7. [PMID: 23749302]
 [5] Mass spectrometric analysis of lysine ubiquitylation reveals promiscuity at site level.
 Danielsen JM, Sylvestersen KB, Bekker-Jensen S, Szklarczyk D, Poulsen JW, Horn H, Jensen LJ, Mailand N, Nielsen ML.
 Mol Cell Proteomics. 2011 Mar;10(3):M110.003590. [PMID: 21139048]
 [6] hCKSAAP_UbSite: improved prediction of human ubiquitination sites by exploiting amino acid pattern and properties.
 Chen Z, Zhou Y, Song J, Zhang Z.
 Biochim Biophys Acta. 2013 Aug;1834(8):1461-7. [PMID: 23603789
Functional Description
  
Sequence Annotation
  
Keyword
 Complete proteome; Reference proteome. 
Sequence Source
 UniProt (SWISSPROT/TrEMBL); GenBank; EMBL 
Protein Length
 187 AA 
Protein Sequence
MTSREHQVSL CNCVPLLRRL LCDAPWRKAR PLHALSRYFR SRVSPSKMAE EPQSVLQLPT 60
SIAAGGEGLT DVSPETTTPE PPSSAAVSPG TEEPAGDTKK KIDILLKAVG DTPIMKTKKW 120
AVERTRTIQG LIDFIKKFLK LVASEQLFIY VNQSFAPSPD QEVGTLYECF GSDGKLVLHY 180
CKSQAWG 187 
Gene Ontology
 GO:0005737; C:cytoplasm; IEA:InterPro.
 GO:0000045; P:autophagic vacuole assembly; IEA:InterPro. 
Interpro
 IPR007242; Atg12. 
Pfam
 PF04110; APG12 
SMART
  
PROSITE
  
PRINTS