UniProt Accession

 DPOLB_RAT; P06766; Q4G081 

Genbank Protein ID

 J02776; U38801; BC098668; M13961 

Genbank Nucleotide ID

 AAA41901.1; AAB00389.1; AAH98668.1; AAA41900.1 

Protein Name

 DNA polymerase beta 

Protein Synonyms/Alias

  

Gene Name

 Polb 

Gene Synonyms/Alias

  

Created Date

 July 27, 2013 

Organism

 Rattus norvegicus (Rat) 

NCBI Taxa ID

 10116 

Lysine Modification

Position	Peptide	Type	References
72	VGTKIAEKIDEFLAT	acetylation	[1]

Reference

 [1] Proteomic analysis of lysine acetylation sites in rat tissues reveals organ specificity and subcellular patterns.
 Lundby A, Lage K, Weinert BT, Bekker-Jensen DB, Secher A, Skovgaard T, Kelstrup CD, Dmytriyev A, Choudhary C, Lundby C, Olsen JV.
 Cell Rep. 2012 Aug 30;2(2):419-31. [PMID: 22902405] 

Functional Description

 Repair polymerase that plays a key role in base-excision repair. Has 5'-deoxyribose-5-phosphate lyase (dRP lyase) activity that removes the 5' sugar phosphate and also acts as a DNA polymerase that adds one nucleotide to the 3' end of the arising single-nucleotide gap. Conducts 'gap-filling' DNA synthesis in a stepwise distributive fashion rather than in a processive fashion as for other DNA polymerases. 

Sequence Annotation

 REGION 183 192 DNA binding.
 ACT_SITE 72 72 Schiff-base intermediate with DNA (By
 METAL 101 101 Sodium; via carbonyl oxygen.
 METAL 103 103 Sodium; via carbonyl oxygen.
 METAL 106 106 Sodium; via carbonyl oxygen.
 METAL 190 190 Magnesium 1.
 METAL 190 190 Magnesium 2.
 METAL 192 192 Magnesium 1.
 METAL 192 192 Magnesium 2.
 METAL 256 256 Magnesium 2.
 MOD_RES 83 83 Omega-N-methylarginine; by PRMT6 (By
 MOD_RES 152 152 Omega-N-methylarginine; by PRMT6 (By
 CROSSLNK 41 41 Glycyl lysine isopeptide (Lys-Gly)
 CROSSLNK 61 61 Glycyl lysine isopeptide (Lys-Gly)
 CROSSLNK 81 81 Glycyl lysine isopeptide (Lys-Gly)  

Keyword

 3D-structure; Complete proteome; Cytoplasm; Direct protein sequencing; DNA damage; DNA repair; DNA replication; DNA synthesis; DNA-binding; DNA-directed DNA polymerase; Isopeptide bond; Lyase; Magnesium; Metal-binding; Methylation; Nucleotidyltransferase; Nucleus; Reference proteome; Sodium; Transferase; Ubl conjugation. 

Sequence Source

 UniProt (SWISSPROT/TrEMBL); GenBank; EMBL 

Protein Length

 335 AA 

Protein Sequence

Gene Ontology

 GO:0005737; C:cytoplasm; ISS:UniProtKB.
 GO:0005634; C:nucleus; IDA:RGD.
 GO:0005876; C:spindle microtubule; IEA:Compara.
 GO:0003684; F:damaged DNA binding; IDA:RGD.
 GO:0003887; F:DNA-directed DNA polymerase activity; ISS:UniProtKB.
 GO:0016829; F:lyase activity; ISS:UniProtKB.
 GO:0046872; F:metal ion binding; IDA:UniProtKB.
 GO:0006284; P:base-excision repair; ISS:UniProtKB.
 GO:0008219; P:cell death; IEA:Compara.
 GO:0006260; P:DNA replication; IEA:UniProtKB-KW.
 GO:0043066; P:negative regulation of apoptotic process; IEA:Compara.
 GO:0006290; P:pyrimidine dimer repair; IDA:RGD.
 GO:0045471; P:response to ethanol; IEP:RGD. 

Interpro

 IPR002054; DNA-dir_DNA_pol_X.
 IPR019843; DNA_pol-X_BS.
 IPR010996; DNA_pol_b-like_N.
 IPR018944; DNA_pol_lambd_fingers_domain.
 IPR022312; DNA_pol_X.
 IPR002008; DNA_pol_X_beta-like.
 IPR003583; Hlx-hairpin-Hlx_DNA-bd_motif.
 IPR027420; PolB_N. 

Pfam

 PF10391; DNA_pol_lambd_f 

SMART

 SM00278; HhH1
 SM00483; POLXc 

PROSITE

 PS00522; DNA_POLYMERASE_X 

PRINTS

 PR00869; DNAPOLX.
 PR00870; DNAPOLXBETA.