CPLM 1.0 - Compendium of Protein Lysine Modification
TagContent
CPLM ID CPLM-004219
UniProt Accession
Genbank Protein ID
Genbank Nucleotide ID
Protein Name
 Glucosidase 2 subunit beta 
Protein Synonyms/Alias
 80K-H protein; Glucosidase II subunit beta; Protein kinase C substrate 60.1 kDa protein heavy chain; PKCSH 
Gene Name
 PRKCSH 
Gene Synonyms/Alias
 G19P1 
Created Date
 July 27, 2013 
Organism
 Homo sapiens (Human) 
NCBI Taxa ID
 9606 
Lysine Modification
Position
Peptide
Type
References
142REGFRLKKILIEDWKubiquitination[1]
167IELQAGKKSLEDQVEubiquitination[1]
376AAQEARNKFEEAERSubiquitination[1]
385EEAERSLKDMEESIRubiquitination[1]
438KLVSQKPKLGGSPTSubiquitination[2]
459WIGPDHDKFSAMKYEubiquitination[1]
464HDKFSAMKYEQGTGCubiquitination[1]
487TVRLLCGKETMVTSTubiquitination[1, 2]
Reference
 [1] Refined preparation and use of anti-diglycine remnant (K-ε-GG) antibody enables routine quantification of 10,000s of ubiquitination sites in single proteomics experiments.
 Udeshi ND, Svinkina T, Mertins P, Kuhn E, Mani DR, Qiao JW, Carr SA.
 Mol Cell Proteomics. 2013 Mar;12(3):825-31. [PMID: 23266961]
 [2] A proteome-wide, quantitative survey of in vivo ubiquitylation sites reveals widespread regulatory roles.
 Wagner SA, Beli P, Weinert BT, Nielsen ML, Cox J, Mann M, Choudhary C.
 Mol Cell Proteomics. 2011 Oct;10(10):M111.013284. [PMID: 21890473] 
Functional Description
 Regulatory subunit of glucosidase II. 
Sequence Annotation
 DOMAIN 209 244 EF-hand 1.
 DOMAIN 245 290 EF-hand 2.
 DOMAIN 413 468 PRKCSH.
 MOTIF 525 528 Prevents secretion from ER (Potential).
 MOD_RES 89 89 Phosphoserine; by PKC (Potential).
 MOD_RES 383 383 Phosphoserine; by PKC (Potential).
 MOD_RES 390 390 Phosphoserine; by PKC (Potential).
 MOD_RES 434 434 Phosphoserine; by PKC (Potential).
 CARBOHYD 72 72 N-linked (GlcNAc...) (Potential).
 CARBOHYD 476 476 N-linked (GlcNAc...) (Potential).  
Keyword
 Alternative splicing; Calcium; Complete proteome; Direct protein sequencing; Endoplasmic reticulum; Glycoprotein; Metal-binding; Phosphoprotein; Polymorphism; Reference proteome; Repeat; Signal. 
Sequence Source
 UniProt (SWISSPROT/TrEMBL); GenBank; EMBL 
Protein Length
 528 AA 
Protein Sequence
MLLPLLLLLP MCWAVEVKRP RGVSLTNHHF YDESKPFTCL DGSATIPFDQ VNDDYCDCKD 60
GSDEPGTAAC PNGSFHCTNT GYKPLYIPSN RVNDGVCDCC DGTDEYNSGV ICENTCKEKG 120
RKERESLQQM AEVTREGFRL KKILIEDWKK AREEKQKKLI ELQAGKKSLE DQVEMLRTVK 180
EEAEKPEREA KEQHQKLWEE QLAAAKAQQE QELAADAFKE LDDDMDGTVS VTELQTHPEL 240
DTDGDGALSE AEAQALLSGD TQTDATSFYD RVWAAIRDKY RSEALPTDLP APSAPDLTEP 300
KEEQPPVPSS PTEEEEEEEE EEEEEAEEEE EEEDSEEAPP PLSPPQPASP AEEDKMPPYD 360
EQTQAFIDAA QEARNKFEEA ERSLKDMEES IRNLEQEISF DFGPNGEFAY LYSQCYELTT 420
NEYVYRLCPF KLVSQKPKLG GSPTSLGTWG SWIGPDHDKF SAMKYEQGTG CWQGPNRSTT 480
VRLLCGKETM VTSTTEPSRC EYLMELMTPA ACPEPPPEAP TEDDHDEL 528 
Gene Ontology
 GO:0005788; C:endoplasmic reticulum lumen; TAS:Reactome.
 GO:0005509; F:calcium ion binding; IEA:InterPro.
 GO:0003723; F:RNA binding; IEA:Compara.
 GO:0045087; P:innate immune response; TAS:Reactome.
 GO:0007243; P:intracellular protein kinase cascade; NAS:UniProtKB.
 GO:0006491; P:N-glycan processing; IEA:InterPro.
 GO:0010977; P:negative regulation of neuron projection development; IEA:Compara.
 GO:0043687; P:post-translational protein modification; TAS:Reactome.
 GO:0006457; P:protein folding; TAS:Reactome.
 GO:0051291; P:protein heterooligomerization; IEA:Compara.
 GO:0018279; P:protein N-linked glycosylation via asparagine; TAS:Reactome. 
Interpro
 IPR018247; EF_Hand_1_Ca_BS.
 IPR002048; EF_hand_dom.
 IPR026874; Glucosidase_2_bsu.
 IPR002172; LDrepeatLR_classA_rpt.
 IPR009011; Man6P_isomerase_rcpt-bd_dom. 
Pfam
 PF13202; EF_hand_3 
SMART
 SM00192; LDLa 
PROSITE
 PS00018; EF_HAND_1
 PS50222; EF_HAND_2
 PS00014; ER_TARGET 
PRINTS